Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   J8Z27_RS14405 Genome accession   NZ_CP072680
Coordinates   3055212..3056588 (-) Length   458 a.a.
NCBI ID   WP_252035469.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43186     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3050212..3061588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J8Z27_RS14385 (J8Z27_14310) dam 3050968..3051795 (-) 828 WP_252035467.1 adenine-specific DNA-methyltransferase -
  J8Z27_RS14390 (J8Z27_14315) - 3051865..3053370 (-) 1506 WP_252035468.1 AAA family ATPase -
  J8Z27_RS14395 (J8Z27_14320) aroB 3053412..3054500 (-) 1089 WP_239870685.1 3-dehydroquinate synthase -
  J8Z27_RS14400 (J8Z27_14325) aroK 3054532..3055050 (-) 519 WP_239838680.1 shikimate kinase AroK -
  J8Z27_RS14405 (J8Z27_14330) pilQ 3055212..3056588 (-) 1377 WP_252035469.1 type IV pilus secretin PilQ family protein Machinery gene
  J8Z27_RS14410 (J8Z27_14335) - 3056595..3057089 (-) 495 WP_252035470.1 pilus assembly protein PilP -
  J8Z27_RS14415 (J8Z27_14340) pilO 3057079..3057660 (-) 582 WP_252035471.1 type 4a pilus biogenesis protein PilO -
  J8Z27_RS14420 (J8Z27_14345) - 3057653..3058210 (-) 558 WP_252035472.1 PilN domain-containing protein -
  J8Z27_RS14425 (J8Z27_14350) pilM 3058200..3059117 (-) 918 WP_252035473.1 type IV pilus assembly protein PilM -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49783.07 Da        Isoelectric Point: 5.8975

>NTDB_id=555047 J8Z27_RS14405 WP_252035469.1 3055212..3056588(-) (pilQ) [Vibrio sp. SCSIO 43186]
MMKVFCIVLLSLIAILKDANAQIHSPSRSEVESEVHSHSTLSVNFQDIPVRKVLQQIADHNNFNLVVSDAVTGNLTLRLK
DIPWPQLLDIILHMEGLEKRMKGNVLLVAPATEIAQLKQQQLEQVKVSSERQPLTSEIISIRFAKAADIAEMLGAQAKTN
MLSERGSIAIDTRTNSLLIRDVASHIQAMRSIITALDVPVKQVQIEARIVSLNEGDLQELGVRWGFSSTNGSHSVGGSIE
SNVEKLNLYGDSGVSLDDFLNVNLAATSTTASTIAFQVAKLASDTLLDLELSALQRESRAEVISSPRLMTTNKQPAYIEQ
GTEIPYLEAASSGATSVNFKKAVLSLKVTPQITSDNRLILDLNVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETI
VLGGIYQHSVSDTVDQVPILGDIPLLGALFRRTYQQMGKSELLIFVTPKVVVPSSINR

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=555047 J8Z27_RS14405 WP_252035469.1 3055212..3056588(-) (pilQ) [Vibrio sp. SCSIO 43186]
ATGATGAAGGTATTTTGCATTGTATTACTTAGTTTGATCGCGATTTTGAAAGATGCTAATGCTCAAATTCACTCTCCGAG
TCGTTCTGAGGTGGAGAGTGAAGTCCATAGTCACAGCACCTTATCTGTTAATTTTCAGGATATCCCTGTTCGTAAAGTGT
TACAGCAAATAGCAGATCACAATAATTTTAATCTGGTTGTTTCGGATGCCGTTACGGGTAACCTGACATTGAGATTAAAG
GATATCCCCTGGCCCCAGTTGTTGGATATTATCCTTCATATGGAAGGGCTTGAAAAACGCATGAAAGGAAATGTTTTACT
GGTGGCGCCAGCGACCGAAATCGCGCAGCTTAAGCAGCAACAGTTGGAGCAGGTGAAAGTCTCATCAGAGCGACAGCCAC
TCACGTCAGAAATTATCTCTATACGCTTTGCTAAAGCGGCAGATATAGCCGAGATGTTAGGTGCACAGGCAAAGACGAAC
ATGCTGTCTGAGCGTGGTTCGATTGCGATTGATACCCGGACCAATAGCCTACTTATTCGAGATGTTGCCAGCCATATTCA
AGCAATGAGGTCTATCATCACCGCCTTAGATGTCCCGGTGAAGCAAGTCCAAATAGAAGCGCGTATCGTCAGTTTGAACG
AAGGCGATTTACAAGAGTTGGGTGTGCGTTGGGGGTTCAGTTCTACCAACGGGAGCCACTCGGTTGGAGGATCGATCGAA
AGCAATGTAGAGAAGCTCAATTTATATGGGGACAGTGGTGTGTCACTGGATGATTTTTTAAACGTCAATTTGGCCGCAAC
TTCTACCACCGCGTCGACCATTGCATTTCAAGTGGCCAAATTGGCCAGTGATACTTTGCTTGATCTTGAGTTGTCTGCAT
TACAAAGAGAGTCGCGGGCAGAGGTGATCTCCAGCCCGCGTTTAATGACGACCAACAAACAGCCCGCTTATATTGAACAA
GGAACCGAAATTCCGTACTTAGAAGCCGCATCGAGTGGCGCAACTAGTGTTAACTTCAAAAAAGCAGTACTGAGCTTGAA
AGTGACGCCGCAGATTACCTCTGATAATCGCTTAATCTTAGATCTGAATGTTACGCAGGACAGGCCGGGCCAAGTTGTAA
AAACGGGTACGGGAGAAGCGGTGGCGATAGATACGCAACGCATTGGGACTCAAGTATTGGTGAATAACGGTGAAACGATT
GTGTTAGGCGGGATTTACCAACACAGTGTGTCTGATACTGTTGATCAGGTGCCGATTTTAGGTGATATTCCCCTGTTGGG
AGCCCTTTTTCGACGAACTTATCAGCAAATGGGTAAAAGTGAATTGCTGATTTTCGTTACTCCAAAAGTGGTCGTTCCGT
CATCAATCAACAGATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(137-202)

Bacterial type II and III secretion system protein (Secretin)

(293-451)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

68.636

96.07

0.659
  pilQ Vibrio cholerae strain A1552

71.359

89.956

0.642
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

71.359

89.956

0.642
  pilQ Legionella pneumophila strain Lp02

46.262

93.45

0.432
  pilQ Legionella pneumophila strain ERS1305867

46.262

93.45

0.432
  pilQ Pseudomonas aeruginosa PAK

41.685

98.472

0.41
  comQ Acinetobacter baylyi ADP1

42.152

97.38

0.41
  pilQ Acinetobacter baumannii D1279779

39.262

100

0.395
  pilQ Acinetobacter baumannii strain A118

39.262

100

0.395
  comE Haemophilus influenzae Rd KW20

41.809

89.301

0.373
  comE Haemophilus influenzae 86-028NP

41.278

88.865

0.367