Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   MS6_RS12480 Genome accession   NZ_AP014524
Coordinates   2705627..2707831 (-) Length   734 a.a.
NCBI ID   WP_001247859.1    Uniprot ID   -
Organism   Vibrio cholerae MS6     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2700627..2712831
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MS6_RS12460 (MS6_2390) hslU 2701500..2702831 (-) 1332 WP_001293366.1 HslU--HslV peptidase ATPase subunit -
  MS6_RS12465 (MS6_2391) hslV 2702897..2703454 (-) 558 WP_000208249.1 ATP-dependent protease subunit HslV -
  MS6_RS12470 (MS6_2392) - 2703591..2704142 (-) 552 WP_001884022.1 SPOR domain-containing protein -
  MS6_RS12475 (MS6_2394) cytR 2704314..2705321 (-) 1008 WP_000224452.1 DNA-binding transcriptional regulator CytR Regulator
  MS6_RS12480 (MS6_2395) priA 2705627..2707831 (-) 2205 WP_001247859.1 primosomal protein N' Machinery gene
  MS6_RS12485 rpmE 2708086..2708304 (+) 219 WP_000643446.1 50S ribosomal protein L31 -
  MS6_RS12490 (MS6_2398) - 2708612..2709889 (+) 1278 WP_001278588.1 malic enzyme-like NAD(P)-binding protein -
  MS6_RS12495 (MS6_2399) metJ 2710000..2710317 (-) 318 WP_000796267.1 met regulon transcriptional regulator MetJ -
  MS6_RS12500 (MS6_2400) - 2710585..2711751 (+) 1167 WP_000213241.1 O-succinylhomoserine (thiol)-lyase -

Sequence

Protein


Download         Length: 734 a.a.        Molecular weight: 82096.38 Da        Isoelectric Point: 8.4853

>NTDB_id=55481 MS6_RS12480 WP_001247859.1 2705627..2707831(-) (priA) [Vibrio cholerae MS6]
MRPSIARVCLPVPLDKSFDYLIPAHLFPVLGGRVQVPFGRQTLVGIVQSLTHQSDFPIEQLKSVQAVLDDAPVWPDKLQS
LLHWCSQFYHYPLGETYANALPGALRKGKAAELTSHKEWRLTALGQEQLMQGVKRGAVKQAQVLHLLQHGALSHQTLLDE
EVSSTTLKALVEKGWIECEERKPVARPWPQELEAKVDKPRLNQEQAIAIAAVNSQQGFGCFLLEGVTGSGKTEVYLNLIT
PVLARGEQALVLVPEIGLTPQTINRFRQRFNVPVEVMHSALNDTERLNAWLAARDKVAGIVIGTRSALLTPFAKLGIIIV
DEEHDSSYKQQDSLRYHARDVAVMRAHLENIPIVLGSATPALETLHNALSGKYHHLQLTQRAGNALPTRNHALDVKGLYL
ESGLSAPLIAEMRRHLSAGNQVMLFLNRRGFSPALMCHECGWIAECQRCDAYYTYHQHSNEMRCHHCGSQRPVLHQCKGC
GSTQLVTVGVGTEQLEAQLHTLFPEYRSVRIDRDSTRRKGSLESALTAIRKGEYQILIGTQMLAKGHHFPDVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLHKDYHHFALTALEERKLAQLPPYSFLSLF
RAEANHTAQVEDFLRQVRETLRCNPWFDSECMVLGPTPAPLAKRAGKFRWQLLLQTPNRTLMQKILQSARPALQQLPLSS
KVRWSIDIDPQDLS

Nucleotide


Download         Length: 2205 bp        

>NTDB_id=55481 MS6_RS12480 WP_001247859.1 2705627..2707831(-) (priA) [Vibrio cholerae MS6]
ATGCGACCCTCCATTGCCCGAGTTTGCCTACCTGTACCTCTGGATAAGTCTTTCGACTATCTGATCCCAGCTCATCTATT
TCCAGTGCTGGGCGGTCGTGTGCAAGTGCCTTTTGGGCGACAAACCTTGGTCGGTATTGTCCAGTCGCTGACACATCAAT
CCGATTTTCCTATCGAACAACTGAAATCCGTGCAAGCCGTTTTGGATGACGCGCCCGTGTGGCCAGATAAACTGCAGTCT
CTGCTGCACTGGTGTAGCCAGTTTTATCATTATCCGCTCGGTGAAACGTACGCGAATGCCTTGCCCGGCGCACTGCGCAA
AGGCAAAGCGGCAGAGCTGACTAGCCATAAAGAGTGGCGTTTGACCGCGCTTGGTCAAGAGCAACTGATGCAAGGGGTGA
AGCGTGGCGCGGTAAAACAAGCGCAAGTTCTCCATTTGTTGCAGCACGGCGCTTTATCGCACCAAACTTTGCTTGATGAA
GAGGTGAGCAGCACGACCCTCAAAGCCTTGGTCGAAAAAGGGTGGATTGAGTGTGAAGAGCGTAAACCTGTGGCGCGTCC
TTGGCCGCAAGAGCTGGAAGCTAAAGTCGATAAACCTAGGCTTAATCAAGAGCAGGCGATCGCGATTGCGGCGGTGAATA
GCCAGCAAGGTTTCGGCTGCTTTTTATTAGAAGGGGTAACGGGCTCAGGTAAAACAGAGGTGTATCTTAACCTCATCACA
CCGGTATTGGCGCGTGGTGAACAAGCCTTGGTGTTGGTGCCGGAAATTGGTTTAACTCCACAAACCATCAATCGTTTTCG
CCAACGTTTTAATGTCCCCGTCGAAGTCATGCACTCGGCGCTCAACGACACTGAACGCCTCAACGCTTGGCTGGCGGCGC
GCGATAAAGTGGCGGGGATTGTGATTGGCACACGCTCGGCGCTGCTCACACCCTTTGCCAAATTGGGCATTATTATTGTT
GATGAAGAACATGATAGTTCTTACAAGCAGCAAGATAGTCTGCGTTATCACGCCCGAGATGTCGCTGTGATGCGCGCGCA
TTTGGAAAACATTCCGATCGTGCTTGGCTCGGCGACGCCAGCCTTGGAAACGTTGCATAATGCGCTCAGTGGTAAATACC
ATCACTTGCAACTGACGCAGCGTGCTGGTAATGCACTACCCACACGTAACCATGCGTTGGATGTGAAAGGGCTCTATCTG
GAAAGTGGTCTGTCTGCGCCTTTGATTGCGGAAATGCGCCGCCATTTGTCGGCGGGCAATCAGGTGATGCTGTTTTTAAA
CCGCCGCGGTTTTTCTCCGGCCTTGATGTGTCACGAGTGTGGCTGGATTGCGGAGTGCCAGCGCTGTGATGCCTATTACA
CCTACCATCAACACAGCAATGAAATGCGCTGCCACCATTGTGGTTCACAGCGTCCTGTTCTCCATCAATGCAAAGGGTGC
GGCTCGACTCAACTGGTTACTGTCGGCGTTGGTACGGAACAGCTTGAAGCGCAATTACACACTCTATTCCCTGAGTATCG
TTCAGTGCGGATTGATCGAGATAGCACGCGGCGTAAAGGCAGTTTGGAATCGGCGTTAACCGCGATTCGCAAAGGCGAAT
ATCAGATTTTGATTGGTACGCAAATGCTCGCCAAAGGACATCACTTCCCCGATGTTACCTTGGTCGCGTTGTTGGATGTC
GATGGCTCATTGTACAGCAGTGATTTTCGCGCTTCTGAGCGCTTGGCTCAGCTCTTTATTCAAGTCGCAGGGCGGGCAGG
GCGTGCCAGCAAGCCGGGCGAAGTGGTGCTACAAACGCATCATCCCGAACACAGTTTGCTACAAGCCTTGCTGCATAAGG
ATTACCACCATTTTGCCCTGACGGCGTTGGAAGAGCGTAAGCTGGCGCAGTTGCCCCCTTATTCTTTCTTGAGCTTATTT
CGAGCTGAAGCCAATCACACCGCTCAAGTGGAAGATTTTTTGCGTCAAGTGCGCGAAACCTTGCGCTGCAATCCTTGGTT
TGATAGCGAATGTATGGTGCTCGGGCCAACGCCTGCGCCACTCGCGAAACGAGCGGGAAAATTCCGTTGGCAACTTTTAC
TACAAACGCCGAATCGCACCCTGATGCAGAAGATTTTGCAAAGCGCACGTCCAGCACTGCAACAATTACCGCTGTCGAGC
AAGGTGCGCTGGTCGATCGATATTGATCCGCAAGATTTGAGCTAA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(204-364)

Primosomal protein N C-terminal domain (PriA_C)

(633-730)

3DNA-binding domain (3BD) (PriA_3primeBD)

(7-102)

Helicase conserved C-terminal domain (Helicase_C)

(515-589)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(446-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

43.572

100

0.439

Multiple sequence alignment