Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   J8I85_RS13170 Genome accession   NZ_CP072601
Coordinates   2830149..2831654 (+) Length   501 a.a.
NCBI ID   WP_209821109.1    Uniprot ID   -
Organism   Burkholderia latens strain AU0505     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2825149..2836654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J8I85_RS13145 (J8I85_13145) kdsD 2826389..2827372 (+) 984 WP_069241758.1 arabinose 5-phosphate isomerase KdsD -
  J8I85_RS13150 (J8I85_13150) - 2827372..2827908 (+) 537 WP_040141999.1 HAD family hydrolase -
  J8I85_RS13155 (J8I85_13155) lptC 2827912..2828526 (+) 615 WP_040142002.1 LPS export ABC transporter periplasmic protein LptC -
  J8I85_RS13160 (J8I85_13160) lptA 2828547..2829212 (+) 666 WP_040142004.1 lipopolysaccharide transport periplasmic protein LptA -
  J8I85_RS13165 (J8I85_13165) lptB 2829209..2829985 (+) 777 WP_040142006.1 LPS export ABC transporter ATP-binding protein -
  J8I85_RS13170 (J8I85_13170) rpoN/rpoN1 2830149..2831654 (+) 1506 WP_209821109.1 RNA polymerase factor sigma-54 Machinery gene
  J8I85_RS13175 (J8I85_13175) raiA 2831814..2832173 (+) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  J8I85_RS13180 (J8I85_13180) - 2832369..2832881 (+) 513 WP_040142010.1 PTS sugar transporter subunit IIA -
  J8I85_RS13185 (J8I85_13185) hprK 2833011..2833979 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  J8I85_RS13190 (J8I85_13190) rapZ 2834055..2834963 (+) 909 WP_040142012.1 RNase adapter RapZ -
  J8I85_RS13195 (J8I85_13195) - 2834991..2835626 (+) 636 WP_040142014.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54710.82 Da        Isoelectric Point: 4.8237

>NTDB_id=554526 J8I85_RS13170 WP_209821109.1 2830149..2831654(+) (rpoN/rpoN1) [Burkholderia latens strain AU0505]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPAGQNAEPSA
SNGSTQSSDRAERDEPAGADEYDGYASGDAGDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTASLDEVLADLPPELEIDGDELNAALALLHSFDPPGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVMP
RLRINDLYANILRNSRGDPAAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=554526 J8I85_RS13170 WP_209821109.1 2830149..2831654(+) (rpoN/rpoN1) [Burkholderia latens strain AU0505]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTAGCGCTGACCCCGCAACTTCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAATCCGCTGCTCGAGAACGATGACGAATGGA
TCGCGAGTCCGCTGCGGGTCGCCGCGGACGGATCGCTGATCGCGCAGACGCCTGCCGGCCAGAATGCCGAACCGAGCGCG
TCCAACGGCAGCACCCAGTCAAGCGATCGCGCCGAACGCGACGAACCTGCCGGCGCCGACGAATACGACGGCTACGCGTC
CGGCGACGCCGGCGACGGCCCGCAGTGGAATCTCGACGAATTCGGCCGTTCGAGCGGCGCATCGGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCGGCGACGTCGCTGCGCGACCACTTGTCCGCGCAATTGCGCGTCACGCAGGCCAGCCCG
CGCGACCGCGCACTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACGGCGTCGCTCGACGAGGTGCT
CGCCGACCTCCCGCCGGAACTGGAGATCGACGGCGACGAGCTGAATGCCGCGCTCGCGCTGCTGCATAGTTTCGACCCGC
CCGGAGTCGGTGCCCGCTCGGCGTCCGAATGCCTGAAGCTGCAGCTGCTGCGCCTTGATCCGTCCCCGACTCGGACGCTC
GCGCTGGAAATCGTGTCGCAGCATCTCGAACTGCTCGCCGCGCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAC
CGACGACGCACTGCGCGACGCGCATGCGCTGATCCGCTCGCTCGAGCCGTTCCCGGGCGCTGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAACTCAATCCGGAGGTGATGCCG
CGCCTGCGGATCAACGACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGGCGGCCGGTTCGCTGAAACAGCA
GCTGCAGGAAGCACGGTGGCTGATCAAGAATATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCGATTGTCG
AGCGTCAAAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGGACACTCGAATTTAAGTACTT
CTTCGGATCACACGTTTCGACCGACACCGGAGGCGCCGCATCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCCAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.775

100

0.695