Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilJ   Type   Machinery gene
Locus tag   J5X98_RS00415 Genome accession   NZ_CP072600
Coordinates   95032..97842 (+) Length   936 a.a.
NCBI ID   WP_223048257.1    Uniprot ID   -
Organism   Leptothermofonsia sichuanensis E412     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 90032..102842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5X98_RS00395 (J5X98_00395) - 92540..93811 (+) 1272 WP_223048253.1 response regulator -
  J5X98_RS00400 (J5X98_00400) pilH 93969..94334 (+) 366 WP_223048254.1 response regulator transcription factor Machinery gene
  J5X98_RS00405 (J5X98_00405) - 94346..94876 (+) 531 WP_223048255.1 chemotaxis protein CheW -
  J5X98_RS00410 (J5X98_00410) - 94893..95048 (+) 156 WP_223048256.1 hypothetical protein -
  J5X98_RS00415 (J5X98_00415) pilJ 95032..97842 (+) 2811 WP_223048257.1 HAMP domain-containing methyl-accepting chemotaxis protein Machinery gene

Sequence

Protein


Download         Length: 936 a.a.        Molecular weight: 100641.74 Da        Isoelectric Point: 3.8881

>NTDB_id=554497 J5X98_RS00415 WP_223048257.1 95032..97842(+) (pilJ) [Leptothermofonsia sichuanensis E412]
MGAGIEQGYQQAQKAYMQGNYEEAAEIVDRLVKEFPDDPSVSLLRGHIYCYGLQQYDIAREQYQSVLALTTNEEFQDYAN
QGLQFINQSEADAGGGGEEVAGSEEGVNDLDDHDIRDFQSATTWQAQDEFATASDFGLDEPDFSLGSDAADFNQSFDLPD
LDFEPPAGEFGSGGFAEEAGTVDTFSPFTDPFAIEEQPDDRLGESPSPFQQTTHMQADDFSSLDEQFAVHDMGRAENDFA
SALDEFDEAFAPLDLPEDPSTHQPFSLESHPSPLDTPTSFQASSNRRTTEDETLFMGGTGLYNQEDQTFGAPDTPSFDAP
TSFDLGAEDSSYYSSEPGDAGSGMVGQSNVDFLDEFDEFDDLGNLSDFDLSENSAGFTSPAVGAGFDLPGSGAVAGGAIA
DRGFSPDADALNFADSGSSLIRDDEVFSISGTSDQVPTFTQLDQQIAEPTPTVEQGAFAFFENASIATKQLITAGVAGVL
SAVAVALAGNLVANYGPGSQREDVVNQIRSTSWVMAAAAMAFGGGSAFFLGQRTAKQVKQSTDDLQNQFHAVCQGNLNAR
ATVYAEDEFGQLATGFNQMARVILTTTSEAQRKAEEQEQAKEDLQRQVIRLLDDVEGAARGDLTVQAEVTADVLGAVADS
FNLTIQNLREIVQQVKTAARQVSKSSTDNEMFARSLSSDALRQAEELAVTLNSVQVMTESIQRVAESAREAEEVARSASA
TALKGGEAVERTVAGILEIRETVAETTRKVKRLAESSQEISKIVALISQIASRTNLLALNASIEAARAGEAGRGFAIVAD
EVRQLADRAAKASKEIEQIVLQIQSETGSVMTAMEEGTQQVIEGTRLAEQAKRSLEDIIQVSNRIDVLVRSITADTVEQT
ETSRAVAQVMQSVELTAQETSQEAQRVSASLQNLVGVARDLLTSVERFRVETIERK

Nucleotide


Download         Length: 2811 bp        

>NTDB_id=554497 J5X98_RS00415 WP_223048257.1 95032..97842(+) (pilJ) [Leptothermofonsia sichuanensis E412]
ATGGGGGCAGGAATTGAACAGGGATATCAACAGGCTCAAAAAGCCTACATGCAGGGTAACTATGAAGAAGCCGCTGAGAT
TGTTGATCGCCTGGTAAAAGAATTTCCCGATGATCCAAGCGTCTCTCTACTGCGGGGGCACATTTATTGCTATGGCTTAC
AGCAGTATGACATTGCCCGGGAGCAGTATCAGTCTGTGCTGGCGTTGACAACCAATGAAGAGTTCCAGGATTATGCCAAC
CAGGGGTTACAGTTCATCAACCAGTCTGAGGCGGATGCAGGTGGCGGCGGAGAAGAGGTTGCTGGATCAGAGGAAGGAGT
GAACGACCTCGATGATCATGACATTAGGGACTTTCAATCTGCAACCACTTGGCAGGCACAGGATGAGTTTGCGACGGCAA
GCGATTTTGGGCTGGACGAACCAGACTTCAGCCTGGGATCGGATGCTGCTGATTTCAACCAGAGTTTTGATCTTCCTGAC
TTAGATTTTGAGCCGCCTGCTGGTGAATTTGGGTCAGGCGGCTTTGCGGAAGAAGCAGGCACTGTTGATACATTTAGTCC
CTTCACAGACCCCTTTGCGATTGAGGAACAGCCTGACGACAGGTTGGGAGAATCGCCATCGCCTTTCCAACAGACCACTC
ACATGCAGGCGGATGATTTCTCTTCCCTGGATGAACAATTTGCTGTCCATGACATGGGCCGTGCAGAGAATGATTTTGCT
TCAGCCCTGGACGAATTTGATGAAGCCTTTGCCCCCCTTGATCTGCCTGAAGATCCGTCCACCCATCAGCCCTTCTCGTT
AGAAAGTCATCCGTCGCCCCTGGATACGCCAACGTCGTTTCAAGCCAGTTCCAATCGCCGGACAACAGAAGATGAAACCC
TGTTCATGGGTGGCACGGGTCTATACAACCAGGAAGACCAGACCTTTGGGGCACCGGATACCCCCTCATTCGATGCCCCC
ACCAGTTTTGATTTAGGTGCAGAGGATAGCTCCTACTATAGTTCTGAACCTGGAGATGCGGGTTCAGGTATGGTTGGGCA
AAGTAATGTTGACTTTCTGGATGAGTTTGACGAGTTTGATGACTTAGGGAACCTGTCAGACTTTGACCTGTCAGAAAACT
CGGCTGGTTTTACCAGTCCTGCGGTTGGCGCAGGATTTGATTTGCCTGGCAGTGGTGCCGTTGCAGGGGGGGCGATCGCC
GATCGGGGGTTTTCCCCAGATGCCGATGCACTCAATTTTGCGGACAGTGGCAGTTCCCTCATCCGTGATGATGAAGTTTT
CAGCATCTCTGGCACCTCCGATCAGGTGCCCACTTTCACCCAACTGGATCAGCAGATTGCGGAGCCGACCCCCACGGTTG
AACAGGGTGCCTTCGCCTTTTTTGAAAATGCCTCGATCGCAACCAAACAATTGATTACGGCTGGCGTGGCGGGCGTCCTG
TCTGCGGTTGCAGTCGCTCTGGCAGGTAATCTGGTCGCTAACTATGGTCCTGGCAGCCAGCGGGAAGATGTGGTCAATCA
GATCCGGAGTACAAGTTGGGTGATGGCAGCGGCTGCCATGGCTTTTGGGGGAGGATCTGCCTTTTTCCTGGGGCAGAGAA
CCGCTAAACAGGTCAAGCAGTCTACAGACGATTTGCAGAATCAGTTTCATGCGGTCTGTCAGGGAAACTTGAATGCTCGT
GCAACGGTTTATGCCGAAGATGAATTTGGTCAGTTGGCAACGGGCTTCAACCAGATGGCGCGGGTTATCCTGACCACAAC
CAGTGAGGCGCAGCGGAAGGCAGAAGAGCAAGAACAGGCGAAGGAAGACCTGCAACGCCAGGTCATTCGGTTACTGGACG
ATGTGGAAGGGGCCGCCCGGGGTGACCTGACGGTGCAGGCAGAGGTGACGGCGGATGTTCTGGGTGCGGTTGCTGACTCG
TTTAACCTGACCATTCAAAACCTGCGCGAAATTGTGCAACAGGTGAAAACGGCGGCTCGCCAGGTGAGCAAGAGTTCAAC
CGACAACGAGATGTTTGCTCGCAGCCTGTCGTCCGATGCGTTGCGGCAGGCCGAGGAACTGGCCGTCACGTTGAACTCTG
TGCAGGTCATGACTGAGTCGATTCAGCGGGTGGCGGAGAGTGCCCGCGAAGCGGAGGAGGTCGCCCGTTCTGCATCGGCA
ACGGCTCTCAAGGGTGGGGAAGCCGTAGAGCGGACGGTTGCCGGTATTCTGGAAATCCGGGAAACCGTGGCGGAAACGAC
CCGCAAGGTGAAGCGACTGGCAGAGTCTTCCCAGGAGATTTCCAAAATTGTAGCGTTGATTTCCCAGATTGCTTCCCGAA
CCAACCTGCTGGCGTTAAACGCTAGTATTGAAGCAGCCCGGGCAGGAGAGGCAGGGCGCGGTTTCGCCATTGTTGCCGAT
GAGGTCCGGCAGTTGGCAGACCGGGCAGCGAAAGCCTCGAAGGAAATTGAACAGATTGTGCTTCAAATCCAGAGTGAAAC
TGGCTCGGTCATGACCGCGATGGAAGAAGGCACCCAGCAGGTGATTGAGGGTACCAGGCTGGCAGAACAGGCGAAGCGAT
CGCTGGAAGACATTATTCAGGTATCTAACCGAATTGATGTCCTGGTGCGATCAATCACAGCAGATACGGTAGAACAGACC
GAAACTTCCCGCGCTGTAGCTCAGGTTATGCAGTCAGTCGAACTGACTGCCCAGGAAACCTCTCAGGAGGCGCAGCGAGT
GTCCGCCTCCCTGCAAAATCTGGTAGGAGTTGCCCGCGATCTGTTAACGTCCGTCGAACGGTTCCGAGTAGAAACGATTG
AACGCAAGTAG

Domains

Predicted by InterproScan.

HAMP domain (HAMP)

(540-581)

Methyl-accepting chemotaxis protein (MCP) signalling domain (MCPsignal)

(713-893)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilJ Synechocystis sp. PCC 6803

46.366

100

0.484