Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   LXM91_RS08725 Genome accession   NZ_CP090477
Coordinates   1777244..1777678 (+) Length   144 a.a.
NCBI ID   WP_012117601.1    Uniprot ID   A7Z4Z1
Organism   Bacillus amyloliquefaciens strain W0101     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1772244..1782678
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LXM91_RS08720 (LXM91_08720) - 1775744..1776955 (-) 1212 WP_039254928.1 cytochrome P450 -
  LXM91_RS08725 (LXM91_08725) nucA/comI 1777244..1777678 (+) 435 WP_012117601.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  LXM91_RS08730 (LXM91_08730) - 1777738..1778493 (+) 756 WP_003154084.1 YoaK family protein -
  LXM91_RS08735 (LXM91_08735) - 1778527..1778889 (-) 363 WP_007410383.1 hypothetical protein -
  LXM91_RS08740 (LXM91_08740) - 1779082..1780410 (-) 1329 WP_039251445.1 S8 family peptidase -
  LXM91_RS08745 (LXM91_08745) - 1780589..1780822 (+) 234 WP_015239897.1 hypothetical protein -
  LXM91_RS08750 (LXM91_08750) - 1781078..1781785 (+) 708 WP_039251443.1 poly-gamma-glutamate hydrolase family protein -
  LXM91_RS08755 (LXM91_08755) - 1781845..1782297 (+) 453 WP_039251441.1 OsmC family protein -
  LXM91_RS08760 (LXM91_08760) - 1782311..1782664 (-) 354 WP_007410379.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15562.48 Da        Isoelectric Point: 7.2418

>NTDB_id=554263 LXM91_RS08725 WP_012117601.1 1777244..1777678(+) (nucA/comI) [Bacillus amyloliquefaciens strain W0101]
MNAFMKWAASLLLVISLQFGFTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=554263 LXM91_RS08725 WP_012117601.1 1777244..1777678(+) (nucA/comI) [Bacillus amyloliquefaciens strain W0101]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCTTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCAGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCGTGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7Z4Z1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

58.974

81.25

0.479