Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   J7649_RS12720 Genome accession   NZ_CP072549
Coordinates   2712778..2713578 (+) Length   266 a.a.
NCBI ID   WP_219308429.1    Uniprot ID   -
Organism   Acinetobacter lwoffii strain H7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2707778..2718578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J7649_RS12690 (J7649_12690) - 2708041..2708451 (+) 411 WP_005107879.1 hypothetical protein -
  J7649_RS12695 (J7649_12695) - 2708723..2710003 (+) 1281 WP_219308426.1 glycosyltransferase family 4 protein -
  J7649_RS12700 (J7649_12700) - 2710024..2710575 (+) 552 WP_005107877.1 phosphatase PAP2 family protein -
  J7649_RS12705 (J7649_12705) iscX 2710663..2710863 (+) 201 WP_004278360.1 Fe-S cluster assembly protein IscX -
  J7649_RS12710 (J7649_12710) ndk 2710974..2711405 (+) 432 WP_004647460.1 nucleoside-diphosphate kinase -
  J7649_RS12715 (J7649_12715) rlmN 2711528..2712763 (+) 1236 WP_004278358.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  J7649_RS12720 (J7649_12720) pilF 2712778..2713578 (+) 801 WP_219308429.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  J7649_RS12725 (J7649_12725) - 2713569..2714360 (+) 792 WP_219308431.1 helix-turn-helix domain-containing protein -
  J7649_RS12730 (J7649_12730) ispG 2714384..2715505 (+) 1122 WP_219308433.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  J7649_RS12735 (J7649_12735) hisS 2715509..2716804 (+) 1296 WP_219308434.1 histidine--tRNA ligase -
  J7649_RS12740 (J7649_12740) - 2716835..2717533 (+) 699 WP_005103250.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30147.29 Da        Isoelectric Point: 9.2878

>NTDB_id=554142 J7649_RS12720 WP_219308429.1 2712778..2713578(+) (pilF) [Acinetobacter lwoffii strain H7]
MRNLTSKFALISTVCVSLVLVACQTPDLGQKDPEKATKVRTQLAVEYLRTGDLDAAKRALDQALETTPRDSQANMMMGVL
LQQEGSKLNLEKADHYFKRAISADPKNAQARNNYGTYLYQLERYNDAIEQFQIAGATLGYDQRFRALENMGRIYLKLGDA
TNAEKSFKQALQANRDSYISMLELAEIFYLNQQFPAATQMYQQFVRGVGQKNQGARALWVGIRTARAGGDQLGMQVLVNQ
LRALFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=554142 J7649_RS12720 WP_219308429.1 2712778..2713578(+) (pilF) [Acinetobacter lwoffii strain H7]
TTGAGAAATCTAACATCAAAGTTTGCTTTGATTTCAACAGTATGTGTGTCTTTGGTTTTAGTCGCGTGTCAGACGCCAGA
TCTGGGTCAGAAAGACCCTGAAAAGGCCACCAAAGTCCGTACCCAATTGGCGGTAGAATATCTGAGAACAGGTGACCTGG
ATGCAGCCAAACGTGCGCTGGATCAGGCACTTGAGACTACTCCGCGTGATTCTCAAGCAAATATGATGATGGGCGTGCTA
CTGCAACAGGAAGGTAGTAAGCTTAATCTCGAAAAAGCAGATCATTATTTCAAGCGTGCGATTTCGGCAGATCCGAAAAA
TGCGCAGGCGCGAAATAATTACGGCACTTATTTATATCAGCTTGAACGCTATAATGATGCGATCGAGCAGTTTCAAATTG
CTGGTGCCACGTTGGGTTATGATCAACGTTTCCGCGCACTGGAAAATATGGGGCGGATTTACCTGAAACTGGGTGATGCA
ACAAATGCTGAAAAATCATTCAAACAGGCGCTGCAAGCCAATCGCGATTCTTATATTTCAATGTTAGAGTTGGCAGAAAT
ATTTTATTTGAACCAGCAGTTCCCGGCGGCGACACAAATGTATCAACAGTTTGTGCGTGGTGTGGGACAGAAGAATCAGG
GTGCTCGTGCGCTCTGGGTCGGCATTCGCACTGCGCGTGCCGGGGGTGATCAGTTGGGTATGCAGGTATTGGTCAACCAG
TTACGTGCACTTTTTCCTGAAAGCCAGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(150-201)

Tetratricopeptide repeat (TPR_17)

(97-129)

Tetratricopeptide repeat (TPR_14)

(40-79)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.077

97.744

0.763
  pilF Acinetobacter baylyi ADP1

69.173

100

0.692