Detailed information NTG0554029

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: J7D37_RS04185
• Genome accession: NZ_CP072526
• Coordinates: 884248..885366 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain DETAB-E227
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 879248..890366

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J7D37_RS04155 (J7D37_04155)	-	880077..880925 (+)	849	WP_000336551.1	class II glutamine amidotransferase	-
J7D37_RS04160 (J7D37_04160)	-	881178..881633 (+)	456	WP_000782976.1	bacteriohemerythrin	-
J7D37_RS04165 (J7D37_04165)	-	881786..882853 (+)	1068	WP_029642282.1	membrane protein	-
J7D37_RS04170 (J7D37_04170)	-	882853..883176 (+)	324	WP_000442584.1	RnfH family protein	-
J7D37_RS04175 (J7D37_04175)	-	883231..883629 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
J7D37_RS04180 (J7D37_04180)	fur	883741..884178 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
J7D37_RS04185 (J7D37_04185)	pilU	884248..885366 (-)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
J7D37_RS04190 (J7D37_04190)	pilT	885394..886431 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
J7D37_RS04195 (J7D37_04195)	-	886558..887250 (+)	693	WP_005129437.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=554029 J7D37_RS04185 WP_000347034.1 884248..885366(-) (pilU) [Acinetobacter baumannii strain DETAB-E227]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=554029 J7D37_RS04185 WP_000347034.1 884248..885366(-) (pilU) [Acinetobacter baumannii strain DETAB-E227]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCTTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTTTTTGAGAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCGCTTAAACATGCTGATTCACCGAACGATTTGCG
TTTAACCATTAAACTTGCAGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39