Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   J7D37_RS02465 Genome accession   NZ_CP072526
Coordinates   529679..530479 (+) Length   266 a.a.
NCBI ID   WP_193787298.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain DETAB-E227     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 524679..535479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J7D37_RS02440 (J7D37_02440) - 525589..526887 (+) 1299 WP_001991191.1 glycosyltransferase family 4 protein -
  J7D37_RS02445 (J7D37_02445) - 526912..527463 (+) 552 WP_000669565.1 phosphatase PAP2 family protein -
  J7D37_RS02450 (J7D37_02450) iscX 527563..527763 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  J7D37_RS02455 (J7D37_02455) ndk 527877..528308 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  J7D37_RS02460 (J7D37_02460) rlmN 528431..529663 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  J7D37_RS02465 (J7D37_02465) pilF 529679..530479 (+) 801 WP_193787298.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  J7D37_RS02470 (J7D37_02470) - 530470..531261 (+) 792 WP_000405163.1 helix-turn-helix domain-containing protein -
  J7D37_RS02475 (J7D37_02475) ispG 531283..532398 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  J7D37_RS02480 (J7D37_02480) hisS 532395..533687 (+) 1293 WP_000095254.1 histidine--tRNA ligase -
  J7D37_RS02485 (J7D37_02485) - 533716..534414 (+) 699 WP_000058788.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30082.39 Da        Isoelectric Point: 9.2307

>NTDB_id=554026 J7D37_RS02465 WP_193787298.1 529679..530479(+) (pilF) [Acinetobacter baumannii strain DETAB-E227]
MSTPKLKTMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=554026 J7D37_RS02465 WP_193787298.1 529679..530479(+) (pilF) [Acinetobacter baumannii strain DETAB-E227]
TTGAGTACTCCGAAGTTGAAGACCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCCAGTGGTTGCCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTACTTTAAGCGTGCAATTTCATCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

74.615

97.744

0.729