Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilH   Type   Machinery gene
Locus tag   J7445_RS03225 Genome accession   NZ_CP072524
Coordinates   678709..679401 (-) Length   230 a.a.
NCBI ID   WP_070656675.1    Uniprot ID   -
Organism   Neisseria sicca strain NS20201025     
Function   type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 673709..684401
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J7445_RS03190 (J7445_03190) panB 673905..674693 (+) 789 WP_070656671.1 3-methyl-2-oxobutanoate hydroxymethyltransferase -
  J7445_RS03195 (J7445_03195) panC 674865..675701 (+) 837 WP_019271720.1 pantoate--beta-alanine ligase -
  J7445_RS03200 (J7445_03200) - 675846..675953 (+) 108 Protein_622 IS5/IS1182 family transposase -
  J7445_RS03205 (J7445_03205) pilL 675979..676470 (-) 492 WP_029609877.1 PilX family type IV pilin Machinery gene
  J7445_RS03210 (J7445_03210) pilK 676488..677066 (-) 579 WP_070656672.1 pilus assembly protein Machinery gene
  J7445_RS03215 (J7445_03215) - 677045..678019 (-) 975 WP_070656673.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  J7445_RS03220 (J7445_03220) pilV 678019..678696 (-) 678 WP_186805565.1 type IV pilus modification protein PilV -
  J7445_RS03225 (J7445_03225) pilH 678709..679401 (-) 693 WP_070656675.1 Tfp pilus assembly protein FimT/FimU Machinery gene
  J7445_RS03230 (J7445_03230) dnaB 679559..680965 (-) 1407 WP_070491795.1 replicative DNA helicase -
  J7445_RS03235 (J7445_03235) - 681133..681714 (+) 582 WP_003777725.1 superoxide dismutase -
  J7445_RS03240 (J7445_03240) - 682232..683860 (-) 1629 WP_003742029.1 ABC-F family ATPase -
  J7445_RS03245 (J7445_03245) - 683959..684153 (+) 195 WP_003742028.1 hypothetical protein -

Sequence

Protein


Download         Length: 230 a.a.        Molecular weight: 25233.68 Da        Isoelectric Point: 9.0302

>NTDB_id=553904 J7445_RS03225 WP_070656675.1 678709..679401(-) (pilH) [Neisseria sicca strain NS20201025]
MYAQQKGFTLIELLVVIAITAIMATIALPNMSEWIASRRAASQAEQVANLLRFARAEAVRLNLPVYVCPVKIRKHGLADG
TCDNSQKSSGLLAYADKNRDKAYKNDTSDISLRSIVLNGTDPAKVSHTFQHVAFGKDNADDGEAVFWSFMPNGTFGYTTS
KTLDKSTTFKYADGYIKISMTDKAATTEETKKSRASIVLIDSSGRVEICAKSDSRPLCQYSESQSPEPKK

Nucleotide


Download         Length: 693 bp        

>NTDB_id=553904 J7445_RS03225 WP_070656675.1 678709..679401(-) (pilH) [Neisseria sicca strain NS20201025]
ATGTACGCACAACAAAAAGGTTTTACCTTAATCGAATTGTTGGTCGTGATTGCTATTACCGCCATCATGGCCACTATCGC
CCTTCCCAATATGAGCGAATGGATTGCCTCCCGCCGCGCTGCCAGTCAGGCAGAGCAGGTGGCAAACCTCTTGCGTTTCG
CACGAGCCGAAGCAGTCCGTCTCAATCTCCCTGTCTATGTCTGTCCCGTAAAAATTAGAAAACACGGTCTGGCTGACGGT
ACATGCGACAACAGCCAAAAAAGCAGCGGCCTGCTCGCCTATGCCGATAAAAACCGAGACAAAGCCTATAAGAATGACAC
AAGCGATATCTCCCTGCGCTCCATAGTCCTCAATGGTACCGACCCTGCCAAAGTTTCCCATACATTCCAACACGTCGCAT
TTGGAAAAGATAATGCCGATGACGGTGAGGCCGTATTTTGGTCATTTATGCCCAACGGCACATTCGGCTATACCACCTCT
AAAACACTCGATAAATCAACGACTTTCAAATACGCCGACGGTTATATCAAGATTAGTATGACTGATAAAGCCGCAACGAC
CGAAGAAACCAAAAAATCCCGCGCCAGTATCGTCCTTATCGACAGCAGTGGTCGCGTCGAAATCTGCGCCAAATCAGATT
CGCGCCCCCTTTGCCAATATTCAGAATCTCAAAGTCCCGAACCCAAAAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Type II transport protein GspH (GspH)

(43-162)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilH Neisseria gonorrhoeae MS11

57.143

94.348

0.539