Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DT99_RS14655 Genome accession   NZ_CP072520
Coordinates   3160165..3161670 (+) Length   501 a.a.
NCBI ID   WP_034189274.1    Uniprot ID   -
Organism   Burkholderia seminalis strain 869T2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3155165..3166670
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DT99_RS14630 (DT99_014630) kdsD 3156397..3157380 (+) 984 WP_034189278.1 arabinose 5-phosphate isomerase KdsD -
  DT99_RS14635 (DT99_014635) - 3157380..3157916 (+) 537 WP_034189277.1 HAD family hydrolase -
  DT99_RS14640 (DT99_014640) lptC 3157920..3158534 (+) 615 WP_034189276.1 LPS export ABC transporter periplasmic protein LptC -
  DT99_RS14645 (DT99_014645) lptA 3158555..3159220 (+) 666 WP_006483139.1 lipopolysaccharide transport periplasmic protein LptA -
  DT99_RS14650 (DT99_014650) lptB 3159217..3159993 (+) 777 WP_034189275.1 LPS export ABC transporter ATP-binding protein -
  DT99_RS14655 (DT99_014655) rpoN/rpoN1 3160165..3161670 (+) 1506 WP_034189274.1 RNA polymerase factor sigma-54 Machinery gene
  DT99_RS14660 (DT99_014660) raiA 3161829..3162188 (+) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  DT99_RS14665 (DT99_014665) - 3162383..3162895 (+) 513 WP_049034209.1 PTS sugar transporter subunit IIA -
  DT99_RS14670 (DT99_014670) hprK 3163025..3163993 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  DT99_RS14675 (DT99_014675) rapZ 3164069..3164977 (+) 909 WP_034189273.1 RNase adapter RapZ -
  DT99_RS14680 (DT99_014680) - 3165005..3165640 (+) 636 WP_034189272.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54721.84 Da        Isoelectric Point: 4.8711

>NTDB_id=553830 DT99_RS14655 WP_034189274.1 3160165..3161670(+) (rpoN/rpoN1) [Burkholderia seminalis strain 869T2]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAS
ANGSSTSSDRAERDEPAGADDYDGYGSGDGNDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPPGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=553830 DT99_RS14655 WP_034189274.1 3160165..3161670(+) (rpoN/rpoN1) [Burkholderia seminalis strain 869T2]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACCTGGCGCTGACGCCGCAGCTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCCACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGTGTCGCGGCGGACGGATCGCTGATCGCGCAGACGCCGCCCGCGCAGAACACCGAGCCGGCCTCG
GCAAACGGCAGCAGCACGTCCAGCGATCGCGCGGAGCGCGACGAGCCGGCCGGCGCCGACGACTACGACGGTTACGGTTC
CGGCGACGGCAACGACGGCCCGCAATGGAATCTCGACGAATTCGGCCGCGCGAGCGGCGCGTCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAGTCGCTCGACGACGACGGCTACCTGACCGCGACGCTCGACGAGGTGCT
CGCCGACCTGCCGCCCGAGCTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGC
CCGGGGTCGGCGCACGCTCGGCGTCCGAATGCCTGAAGCTGCAGCTGCTGCGCCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCACCTCGAACTGCTCGCGGCCCGCGATTTCACACGGTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGATGCGCATGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGGTGGCTCGCGGAACTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTGAAGCAGCA
GCTCCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGTTTCGACACGATCCTGCGAGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTGGAATTTAAGTACTT
CTTCGGATCTCACGTTTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.182

100

0.689