Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   B938_RS00570 Genome accession   NC_019842
Coordinates   106717..108096 (+) Length   459 a.a.
NCBI ID   WP_015239037.1    Uniprot ID   -
Organism   Bacillus velezensis AS43.3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 101717..113096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B938_RS00550 (B938_00430) ctsR 102068..102532 (+) 465 WP_003156396.1 transcriptional regulator CtsR -
  B938_RS00555 (B938_00435) - 102546..103103 (+) 558 WP_007410387.1 UvrB/UvrC motif-containing protein -
  B938_RS00560 (B938_00440) - 103103..104194 (+) 1092 WP_003156398.1 protein arginine kinase -
  B938_RS00565 (B938_00445) clpC 104191..106623 (+) 2433 WP_015239036.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  B938_RS00570 (B938_00450) radA 106717..108096 (+) 1380 WP_015239037.1 DNA repair protein RadA Machinery gene
  B938_RS00575 (B938_00455) disA 108100..109182 (+) 1083 WP_007410390.1 DNA integrity scanning diadenylate cyclase DisA -
  B938_RS00580 (B938_00460) - 109296..110396 (+) 1101 WP_003156403.1 PIN/TRAM domain-containing protein -
  B938_RS00585 (B938_00465) ispD 110409..111107 (+) 699 WP_015239038.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  B938_RS00590 (B938_00470) ispF 111100..111576 (+) 477 WP_003156407.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49524.94 Da        Isoelectric Point: 7.1316

>NTDB_id=55119 B938_RS00570 WP_015239037.1 106717..108096(+) (radA) [Bacillus velezensis AS43.3]
MAKSKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKAPANRRAAFSHSVQTVQKPSPISSIETSEEPRVQTKLEEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLADTAGSVLYISGEESVKQTKLRADRLGINSQMLHVLSETDMEYIS
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGASGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPEANADGWTIPKGIEVVGVANVAEALRTSLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=55119 B938_RS00570 WP_015239037.1 106717..108096(+) (radA) [Bacillus velezensis AS43.3]
ATGGCTAAATCAAAAACAAAATTCATCTGCCACTCATGCGGATATGAATCCGCAAAATGGATGGGAAAGTGCCCGGGCTG
CGGGGCTTGGAATACAATGGTGGAAGAAACCATAAAAAAAGCGCCGGCGAATCGGCGGGCGGCTTTCTCCCACTCCGTTC
AAACCGTCCAGAAACCATCACCCATTTCATCAATTGAAACATCGGAAGAACCCCGCGTCCAAACAAAGCTCGAAGAATTC
AACAGAGTGCTTGGCGGCGGCGTTGTGAAAGGCTCGTTAGTATTAATCGGCGGCGATCCCGGGATCGGAAAATCAACGCT
CCTTCTCCAAGTATCCGCACAATTAGCAGATACGGCTGGCAGCGTGCTTTACATTTCAGGGGAAGAATCCGTGAAGCAAA
CGAAGCTGCGCGCCGACAGACTTGGAATCAACAGCCAAATGTTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAAACCCGCATTTGTTGTCGTAGACTCCATTCAGACCGTTTACCAAAGTGATATCACCTCAGC
TCCCGGCAGTGTATCACAAGTAAGAGAGTGTACGGCCGAATTGATGAAAATCGCGAAAACAAACGGCATACCTATTTTTA
TCGTCGGGCACGTCACAAAAGAAGGATCGATCGCAGGGCCGAGATTGCTTGAGCACATGGTCGATACCGTTCTATACTTT
GAAGGAGAGCGGCACCATACATTCCGTATTTTGCGGGCCGTAAAAAACCGTTTCGGCTCCACAAATGAAATGGGCATCTT
TGAAATGCGGGAAGAGGGGCTTACGGAAGTGCTGAACCCTTCTGAAATTTTCTTGGAAGAGCGTTCTGCCGGGGCATCGG
GTTCGAGTATCGTTGCGTCAATGGAAGGAACGAGACCGATCCTGGTTGAAATTCAGGCGCTCATATCACCGACCAGTTTT
GGAAACCCGCGCCGCATGGCTACAGGCATTGACCACAACAGGGTATCGTTAATTATGGCTGTGCTTGAGAAGCGGGTGGG
TCTTTTGCTTCAAAATCAAGATGCGTATTTAAAGGTCGCCGGCGGGGTCAAACTGGATGAACCGGCGATTGATTTGGCAG
TAGCCGTCAGCATTGCTTCAAGCTTCAGAGATACACCGCCGAATCCCGCAGATTGCTTTATCGGTGAAGTGGGTCTGACA
GGAGAAGTCCGCAGGGTGTCAAGAATCGAACAGCGCGTAAAGGAAGCGGCGAAACTCGGCTTCAAGCGCATGATCATACC
TGAGGCGAATGCAGACGGGTGGACAATACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-222)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

93.231

99.782

0.93
  radA Streptococcus pneumoniae Rx1

63.576

98.693

0.627
  radA Streptococcus pneumoniae D39

63.576

98.693

0.627
  radA Streptococcus pneumoniae R6

63.576

98.693

0.627
  radA Streptococcus pneumoniae TIGR4

63.576

98.693

0.627
  radA Streptococcus mitis NCTC 12261

63.576

98.693

0.627
  radA Streptococcus mitis SK321

63.355

98.693

0.625

Multiple sequence alignment