Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   J5V73_RS20975 Genome accession   NZ_CP072057
Coordinates   4092326..4093369 (-) Length   347 a.a.
NCBI ID   WP_113734225.1    Uniprot ID   -
Organism   Bacillus mycoides strain JAS12/5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4087326..4098369
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5V73_RS20930 (J5V73_20925) - 4087724..4087924 (-) 201 WP_002015009.1 YqzE family protein -
  J5V73_RS20935 (J5V73_20930) - 4087963..4088460 (-) 498 WP_002129082.1 shikimate kinase -
  J5V73_RS20940 (J5V73_20935) - 4088612..4089262 (-) 651 WP_113734229.1 2OG-Fe(II) oxygenase -
  J5V73_RS20945 (J5V73_20940) comGG 4089437..4089808 (-) 372 WP_131246343.1 competence type IV pilus minor pilin ComGG -
  J5V73_RS20950 (J5V73_20945) comGF 4089805..4090266 (-) 462 WP_044442604.1 competence type IV pilus minor pilin ComGF -
  J5V73_RS20955 (J5V73_20950) - 4090250..4090546 (-) 297 WP_016100615.1 hypothetical protein -
  J5V73_RS20960 (J5V73_20955) comGD 4090539..4090994 (-) 456 WP_002129089.1 competence type IV pilus minor pilin ComGD -
  J5V73_RS20965 (J5V73_20960) comGC 4090991..4091290 (-) 300 WP_002088623.1 competence type IV pilus major pilin ComGC -
  J5V73_RS20970 (J5V73_20965) comGB 4091302..4092339 (-) 1038 WP_131246342.1 competence type IV pilus assembly protein ComGB -
  J5V73_RS20975 (J5V73_20970) comGA 4092326..4093369 (-) 1044 WP_113734225.1 competence type IV pilus ATPase ComGA Machinery gene
  J5V73_RS20980 (J5V73_20975) - 4093575..4094270 (+) 696 WP_002014995.1 metalloregulator ArsR/SmtB family transcription factor -
  J5V73_RS20985 (J5V73_20980) - 4094550..4094792 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  J5V73_RS20990 (J5V73_20985) - 4094900..4096294 (+) 1395 WP_002138338.1 L-cystine transporter -
  J5V73_RS20995 (J5V73_20990) - 4096375..4096767 (-) 393 WP_060749607.1 hypothetical protein -
  J5V73_RS21000 (J5V73_20995) - 4096855..4097070 (-) 216 WP_002015115.1 DUF3912 family protein -
  J5V73_RS21005 (J5V73_21000) - 4097323..4097634 (+) 312 WP_002165070.1 hypothetical protein -
  J5V73_RS21010 (J5V73_21005) - 4097670..4098158 (-) 489 WP_060749606.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39264.78 Da        Isoelectric Point: 9.4590

>NTDB_id=550293 J5V73_RS20975 WP_113734225.1 4092326..4093369(-) (comGA) [Bacillus mycoides strain JAS12/5]
MNSVELFANMIMKEACRVQASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGKEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKWQTRRIITLEDPVEQRKDGLLQIQINEKAGITYKTGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCATLCKSMRKVRQASIYELLYGYELKQAIKEASGEH
VTYHYKTLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=550293 J5V73_RS20975 WP_113734225.1 4092326..4093369(-) (comGA) [Bacillus mycoides strain JAS12/5]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCAAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCGATTCAATTACGTATTGGAAAAGATTTAATTACGAAACGGTGTATTGAGAAAGAATTTGGAG
AAAAGCTTGTTTCACACTTTAAATTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAACCTCAAAATGGTTCGTTGTAC
TTACAAATTGATGGAAAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGCATCTGTACAGCCACTGTCTCATCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCTCATGGGTTACTCGTATTTACTGGGCCAACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAATGGCAAACACGTCGCATCATTACACTGGAAGATCCAGTTGAGCAAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATAAAACGGGATTAAAGGCTATTTTGCGTCATGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTAATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTAGC
AGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCATTTTGCAGAGGGAAGTGTGCAACTTTATGTAAATCAATGAGGA
AAGTGAGACAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATAAAACGTTGGAATCGTCGGTTAGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.349

100

0.573
  pilB Glaesserella parasuis strain SC1401

43.151

84.15

0.363