Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   J5V73_RS05895 Genome accession   NZ_CP072057
Coordinates   1161410..1164925 (+) Length   1171 a.a.
NCBI ID   WP_016093092.1    Uniprot ID   -
Organism   Bacillus mycoides strain JAS12/5     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1156410..1169925
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5V73_RS05860 (J5V73_05860) - 1156607..1157101 (-) 495 WP_131246099.1 DUF6584 family protein -
  J5V73_RS05865 (J5V73_05865) cspA 1157378..1157581 (+) 204 WP_002011282.1 RNA chaperone/antiterminator CspA -
  J5V73_RS05870 (J5V73_05870) - 1157698..1157883 (-) 186 WP_002064194.1 hypothetical protein -
  J5V73_RS05875 (J5V73_05875) - 1158148..1158729 (+) 582 WP_002064195.1 competence protein ComK -
  J5V73_RS05880 (J5V73_05880) - 1158974..1159570 (+) 597 WP_131246100.1 DUF4306 domain-containing protein -
  J5V73_RS05885 (J5V73_05885) - 1159925..1160518 (+) 594 WP_002140801.1 TVP38/TMEM64 family protein -
  J5V73_RS05890 (J5V73_05890) lepB 1160729..1161292 (+) 564 WP_016119804.1 signal peptidase I -
  J5V73_RS05895 (J5V73_05895) addB 1161410..1164925 (+) 3516 WP_016093092.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  J5V73_RS05900 (J5V73_05900) addA 1164922..1168647 (+) 3726 WP_215576874.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  J5V73_RS05905 (J5V73_05905) - 1168671..1168949 (+) 279 WP_113734008.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  J5V73_RS05910 (J5V73_05910) - 1169165..1169872 (+) 708 WP_113734009.1 DNA alkylation repair protein -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134368.34 Da        Isoelectric Point: 5.1194

>NTDB_id=550272 J5V73_RS05895 WP_016093092.1 1161410..1164925(+) (addB) [Bacillus mycoides strain JAS12/5]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDTHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLIEKLSDSEYVKGAEIYIDGFHSFSPQELEIVRGLMRLGTRVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLETHYEARPNEKYHGEASVTISTAANL
RAEVEGVAREIRRLVADEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKEAMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDASEMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEELRVRAEENGDFLFATDHEQVWEEVMNLLDTFVEMLGEEK
MSLSMFTEVMSTGLEALQFANIPPSLDQVLVANIDHSRLSDIKATFIIGVNEGVIPAAPMDEGMLSDEERNVLTAAGIEL
APTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKESFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTDVSRDLYGDTIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADKLLRENRTWADLSIKECEHLSALVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSLILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFALPNGVKMEVVGRIDRVDKAEDESG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGSTASPAGVLYFHIHNPIVEMKGDASEEEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHSFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=550272 J5V73_RS05895 WP_016093092.1 1161410..1164925(+) (addB) [Bacillus mycoides strain JAS12/5]
ATGTCACTTCGATTTGTGATTGGTAGAGCTGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
ACAGCGCCCAAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATTGGGA
GTGAAGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCGCGATTAGCGTGGAAGGTGCTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCTGGTGTACATATGTTGCTTCGTAAAATTGTAGAATCTCGTAAAGATGGACT
ATCTGTGTTTCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGTAGTATGATTGCTGAGTTTAAACGTTACA
ATGTGACGCCGTCTAACGTATATGAAATGTGGCAACAATTAGACACGCATAGCAGTAGTGCAGAGCAAAAGCTACTAGCG
AATAAAGTGTATGATTTACAGTTACTATATGATGATTTTGAACGTGCTTTAATCGGAAAGTATTTAGATTCAGAAGACTA
CTTACAATTATTAATTGAGAAGCTTTCAGACTCTGAGTATGTAAAAGGCGCGGAAATTTATATAGATGGATTCCATTCAT
TTTCTCCTCAAGAGCTAGAAATCGTTAGAGGGCTAATGAGATTAGGAACAAGAGTAACAATAACATTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTGAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACAATTCCACTTATGGAACAACCGCGTTTTCATTCTCCAGCATTAGCTCATT
TAGAAACGCATTATGAAGCGCGTCCGAATGAAAAGTATCATGGTGAAGCGAGTGTAACAATTAGTACAGCAGCTAATTTA
CGAGCTGAAGTAGAAGGTGTAGCTCGTGAAATTAGAAGACTTGTGGCGGATGAAGACTATCGTTACCGAGATATCGCAGT
CCTTCTTCGTAACGGAGAAAGTTATTACGACGTAATGCGAACGTTATTTACGGATTATAATATCCCGCATTTCATCGATG
AAAAACGCCCAATGTCACATCATCCGCTAGTAGAATGTATTCGTTCGGCACTCGAGATTATTAGCGGGAATTGGCGTTAT
GATGCAGTATTCCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGATGTAAGAAAAGAAGCGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAAGATCCGTGGATGTATCGTCGTT
ATCGTTCTCTTGATGATGCGAGCGAAATGATAACAGACAGTGAGCGTGAAATGGAAGAGAAAATAAATCGACTTCGCGAC
GTTGTAAGAACGCCAGTTATTCGTATGCAAAAAAGACTGAAGCGTGCTGGAACAGTCATGCAAATGTGCGAAGCGGTATA
TTTATTTTTAGAGGAGCTTGACGTTCCGAAAAAACTAGAAGAATTACGTGTTCGTGCAGAAGAAAATGGAGATTTCTTAT
TTGCGACAGACCATGAGCAAGTATGGGAAGAAGTGATGAATCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAG
ATGTCCCTTTCAATGTTTACGGAGGTTATGTCGACAGGTCTTGAGGCACTTCAATTTGCGAACATTCCGCCGTCACTAGA
TCAAGTGTTAGTTGCTAACATTGATCATTCTAGATTATCAGATATTAAAGCGACGTTCATTATTGGAGTAAATGAAGGAG
TGATTCCAGCAGCGCCTATGGATGAAGGGATGCTTTCTGATGAGGAAAGAAATGTTCTCACCGCTGCAGGTATCGAATTA
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTATCAAATGGTGACGAGAGCATCTGAGAAGTTATA
CATTTCCTGTCCGCTTGCGGATGAAGAAGGAAAGACGTTACTTGCTTCTAGTTTTATTAAAAAAATAAAAAGAATGTTCC
CTGATGTGAAAGAATCGTTTATTACGAATGATGTAAATGACTTATCGCGTTCGGAACAAATCTCATATGTAGCAACGCCA
GAAGTAACGTTGTCTTATGTCATGCAGCAACTGCAAACGTGGAAGCGATACGGATTTGAAGGCAATTTAGACTTTTGGTG
GGACGTATATAACTTCTACGTTACTTCAGATGAATGGAAGCAAAAAAGTAGCCGTGTGTTATCAAGTTTATTCTACCGAA
ATCGTGCACAGAAACTAAGTACAGATGTAAGTAGGGATTTATATGGAGATACAATTAAGGGAAGCGTTTCTCGTATGGAA
CTATTTAATCGCTGTGCGTATGCTCATTTTGCTCAGCACGGTTTATCGCTAAGAGAGCGTGATATTTTCAAACTTGATGC
GCCAGATATCGGTGAATTATTCCATGCAGCGCTGAAGAGAATTGCAGACAAGCTATTACGTGAAAATCGGACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAACACCTTTCTGCTTTAGTAATAGAAGAAATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCGAACCGTCATTTCTATTTAAAGCAAAAACTACAACAAATCATTTTCCGTACGTCACTTATTCTTCGTGAACA
TGCGAAGTCGAGTGGATTTGTACCAGTTGATTTAGAAGTACCGTTCGGTATGGGCGGGACTGGATCTCTTCCGCCAATGG
AATTTGCGTTGCCAAATGGTGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCGGAAGATGAGAGTGGT
ACGTTCCTTCGTATTATTGATTATAAATCAAGTTCGAAAGCGTTAGATTTAACAGAAGTGTATTACGGACTCGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAGGGCAGTACTGCATCACCGGCGGGTG
TATTGTATTTCCATATTCATAACCCGATTGTTGAGATGAAAGGCGATGCATCTGAAGAAGAAATTGAGAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGTCTCGTACTAGGAGATGCTGACGTTGTGCGTTTAATGGATAATAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGATGGTAGTTTTAGTGCGCGTTCTAGCATTGCAAGTGAACAAGAGTTTA
ACGTACTTCAAAAATATGTACACCACTCGTTTGAAAATATCGGAAAAGACATTACAGAAGGTGTTATCGATATTGCTCCT
TACAAAAAGGGGAATAAAGCGGCATGTACGTTCTGTAACTTCAAATCTGTTTGTCAATTTGATGAATCACTTGAAGATAA
CCAATTCCGTACGTTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(302-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.147

100

0.492