Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   J5W00_RS25900 Genome accession   NZ_CP072055
Coordinates   5020173..5021522 (-) Length   449 a.a.
NCBI ID   WP_215560806.1    Uniprot ID   -
Organism   Bacillus mycoides strain JAS85/1     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5015173..5026522
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5W00_RS25865 (J5W00_25865) - 5015294..5015989 (-) 696 WP_002129924.1 hypothetical protein -
  J5W00_RS25870 (J5W00_25870) - 5015976..5016764 (-) 789 WP_016121052.1 hypothetical protein -
  J5W00_RS25875 (J5W00_25875) - 5016796..5017380 (-) 585 WP_002068272.1 hypothetical protein -
  J5W00_RS25880 (J5W00_25880) - 5017499..5017993 (-) 495 WP_002184661.1 hypothetical protein -
  J5W00_RS25885 (J5W00_25885) hpf 5018282..5018824 (-) 543 WP_002089603.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  J5W00_RS25890 (J5W00_25890) cspC 5019145..5019342 (-) 198 WP_001990088.1 cold shock protein CspC -
  J5W00_RS25895 (J5W00_25895) - 5019469..5020173 (-) 705 WP_033731070.1 ComF family protein -
  J5W00_RS25900 (J5W00_25900) comFA 5020173..5021522 (-) 1350 WP_215560806.1 ATP-dependent helicase ComFA Machinery gene
  J5W00_RS25905 (J5W00_25905) - 5021653..5022987 (-) 1335 WP_215577368.1 NlpC/P60 family protein -
  J5W00_RS25910 (J5W00_25910) - 5023221..5023535 (-) 315 WP_002089608.1 helix-turn-helix domain-containing protein -
  J5W00_RS25915 (J5W00_25915) - 5023698..5024540 (-) 843 WP_002016143.1 DegV family protein -
  J5W00_RS25920 (J5W00_25920) - 5024779..5025414 (+) 636 WP_002034752.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 50890.22 Da        Isoelectric Point: 9.5338

>NTDB_id=550255 J5W00_RS25900 WP_215560806.1 5020173..5021522(-) (comFA) [Bacillus mycoides strain JAS85/1]
MLAGRQLLLEELSSDLQNKLDHLKKHREVVCVQGVIKKSSKYTCQRCGNIEQRLFASFLCKRCSEVCAYCRKCITMGRVS
ECAVLVRGIAEIQGESYLNPLQWEGVLSDGQELAAQSVVDAVRQKESFFIWAVCGAGKTEMLFRGIAEALQKGERVCIAT
PRTDVVLELAPRLQEVFPNINVAALYGGSLDREKDAALIVATTHQLLRYYRAFHVMIVDEIDAFPYHADKMLQYAVNEAM
KEKAARIYLTATPDEKWKRKLRSGKQKGVIISGRYHRHPLPVPIFRWCGNWKKGLIRKKIPRALLQWLNLYLSKRYPIFL
FVPHVRYVEEISQLLKSLNNQVDGVHAEDPARKEKVAAFRKGEIHLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSSDEPHGDVIYFHYGKTEAMVRAKKHIQSMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=550255 J5W00_RS25900 WP_215560806.1 5020173..5021522(-) (comFA) [Bacillus mycoides strain JAS85/1]
ATGTTAGCTGGTAGACAGTTACTGTTAGAAGAACTCTCTTCAGATTTGCAGAATAAATTGGATCATTTGAAAAAGCACCG
AGAAGTCGTTTGTGTGCAAGGGGTAATTAAGAAGTCTTCAAAATATACGTGTCAGCGCTGCGGAAATATAGAGCAGCGAC
TATTTGCGTCATTTTTATGTAAGAGGTGCAGTGAAGTATGTGCGTATTGCCGTAAGTGTATAACGATGGGAAGGGTCAGT
GAATGTGCTGTACTTGTGCGCGGGATTGCTGAAATACAAGGAGAAAGTTATTTGAATCCGTTACAGTGGGAAGGCGTTTT
GTCTGACGGTCAGGAGTTAGCTGCGCAAAGTGTCGTTGACGCTGTTAGGCAGAAAGAATCATTTTTTATTTGGGCGGTGT
GCGGGGCTGGAAAAACAGAAATGTTGTTTCGCGGAATTGCAGAGGCACTACAAAAGGGAGAAAGAGTTTGTATTGCAACG
CCGAGAACGGACGTTGTACTTGAATTAGCACCAAGATTACAAGAAGTGTTTCCAAATATAAATGTAGCTGCTTTATACGG
AGGGAGTTTAGACCGAGAAAAAGATGCGGCGTTAATCGTTGCAACTACACATCAATTGTTACGTTATTATAGGGCGTTCC
ATGTCATGATTGTAGATGAGATAGATGCTTTTCCGTATCATGCAGATAAGATGTTGCAGTATGCAGTAAACGAAGCGATG
AAAGAGAAGGCAGCACGTATTTATTTAACTGCAACTCCAGATGAAAAGTGGAAGCGTAAGTTGCGAAGTGGTAAGCAAAA
AGGGGTTATTATTTCAGGGCGATACCATCGTCATCCATTGCCAGTTCCGATATTTCGGTGGTGCGGAAATTGGAAAAAGG
GTCTTATACGTAAAAAAATTCCTCGTGCTTTATTACAATGGTTAAATCTGTACTTATCGAAACGATACCCCATTTTTCTA
TTTGTTCCTCATGTGCGATATGTAGAAGAAATTAGCCAGTTATTAAAATCATTAAACAATCAAGTTGACGGTGTGCATGC
AGAAGATCCGGCGAGAAAAGAGAAGGTAGCAGCTTTCAGAAAAGGAGAAATCCATTTATTAGTTACAACGACGATTTTGG
AGCGAGGAGTAACTGTTAAAAATTTACAAGTTGCAGTTTTAGGAGCTGAGGAAGAAATATTTTCAGAAAGTGCTCTCGTT
CAAATTGCAGGGCGTGCTGGCAGAAGCTCTGATGAACCGCATGGAGATGTTATCTATTTTCATTATGGAAAGACAGAGGC
GATGGTGCGTGCGAAAAAACATATTCAAAGTATGAATAAGAATGCTAAAGAACAAGGATTGATAGATTGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(107-254)

Helicase conserved C-terminal domain (Helicase_C)

(313-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

50.113

98.218

0.492
  comFA Latilactobacillus sakei subsp. sakei 23K

40.704

88.641

0.361