Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   J5W00_RS21265 Genome accession   NZ_CP072055
Coordinates   4166750..4167793 (-) Length   347 a.a.
NCBI ID   WP_215560731.1    Uniprot ID   -
Organism   Bacillus mycoides strain JAS85/1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4161750..4172793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5W00_RS21220 (J5W00_21220) - 4162148..4162348 (-) 201 WP_002015009.1 YqzE family protein -
  J5W00_RS21225 (J5W00_21225) - 4162387..4162884 (-) 498 WP_033731265.1 shikimate kinase -
  J5W00_RS21230 (J5W00_21230) - 4163036..4163686 (-) 651 WP_002131210.1 2OG-Fe(II) oxygenase -
  J5W00_RS21235 (J5W00_21235) comGG 4163861..4164232 (-) 372 WP_033731267.1 competence type IV pilus minor pilin ComGG -
  J5W00_RS21240 (J5W00_21240) comGF 4164229..4164690 (-) 462 WP_002129086.1 competence type IV pilus minor pilin ComGF -
  J5W00_RS21245 (J5W00_21245) - 4164674..4164970 (-) 297 WP_002088621.1 hypothetical protein -
  J5W00_RS21250 (J5W00_21250) comGD 4164963..4165418 (-) 456 WP_002129089.1 competence type IV pilus minor pilin ComGD -
  J5W00_RS21255 (J5W00_21255) comGC 4165415..4165714 (-) 300 WP_002088623.1 competence type IV pilus major pilin ComGC -
  J5W00_RS21260 (J5W00_21260) comGB 4165726..4166763 (-) 1038 WP_002129092.1 competence type IV pilus assembly protein ComGB -
  J5W00_RS21265 (J5W00_21265) comGA 4166750..4167793 (-) 1044 WP_215560731.1 competence type IV pilus ATPase ComGA Machinery gene
  J5W00_RS21270 (J5W00_21270) - 4167999..4168694 (+) 696 WP_002014995.1 metalloregulator ArsR/SmtB family transcription factor -
  J5W00_RS21275 (J5W00_21275) - 4168976..4169218 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  J5W00_RS21280 (J5W00_21280) - 4169326..4170720 (+) 1395 WP_215560732.1 L-cystine transporter -
  J5W00_RS21285 (J5W00_21285) - 4170801..4171193 (-) 393 WP_002190672.1 hypothetical protein -
  J5W00_RS21290 (J5W00_21290) - 4171281..4171496 (-) 216 WP_002067106.1 DUF3912 family protein -
  J5W00_RS21295 (J5W00_21295) - 4171749..4172060 (+) 312 WP_002129099.1 hypothetical protein -
  J5W00_RS21300 (J5W00_21300) - 4172097..4172585 (-) 489 WP_002015119.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39306.82 Da        Isoelectric Point: 9.4642

>NTDB_id=550244 J5W00_RS21265 WP_215560731.1 4166750..4167793(-) (comGA) [Bacillus mycoides strain JAS85/1]
MNSVELFANMIMKEACRVRASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKWQTRRIITLEDPVEQRKDGLLQIQINEKAGITYKTGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGTILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCATLCKSMRKVRQASIYELLYGYELKQAIKEASGEH
VTYHYKTLEASVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=550244 J5W00_RS21265 WP_215560731.1 4166750..4167793(-) (comGA) [Bacillus mycoides strain JAS85/1]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCGAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCGATTCAATTACGTATTGGAAAAGATTTAATTACGAAACGGTGTATTGAGAAAGAATTTGGAG
AAAAGCTTGTTTCACACTTTAAATTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAACCTCAAAATGGTTCGTTGTAC
TTACAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGCATCTGTACAGCCACTGTCTCATCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCTCATGGGTTACTCGTATTTACTGGGCCAACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAATGGCAAACACGTCGCATTATTACACTGGAAGATCCAGTTGAGCAAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATAAAACGGGATTAAAGGCTATTTTGCGTCATGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTAATGACAACCTTGCAT
ACAAATGATGCGAAAGGAACGATACTGCGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTAGC
AGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCATTTTGCAGAGGGAAGTGTGCAACTTTGTGTAAATCAATGAGGA
AAGTGAGACAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATAAAACGTTGGAAGCGTCGGTTCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.061

100

0.571