Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ANACY_RS21505 Genome accession   NC_019771
Coordinates   4906838..4907962 (-) Length   374 a.a.
NCBI ID   WP_015216328.1    Uniprot ID   A0A9X1GMH4
Organism   Anabaena cylindrica PCC 7122     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4901838..4912962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ANACY_RS21470 (Anacy_4343) - 4901867..4902580 (-) 714 WP_015216321.1 hypothetical protein -
  ANACY_RS21475 (Anacy_4344) - 4902675..4903013 (-) 339 WP_015216322.1 XisI protein -
  ANACY_RS21480 (Anacy_4345) - 4903020..4903418 (-) 399 WP_015216323.1 PIN domain-containing protein -
  ANACY_RS21485 (Anacy_4346) - 4903435..4903704 (-) 270 WP_015216324.1 hypothetical protein -
  ANACY_RS21490 (Anacy_4347) - 4903821..4904816 (+) 996 WP_015216325.1 hypothetical protein -
  ANACY_RS21495 (Anacy_4348) - 4904824..4905384 (-) 561 WP_015216326.1 hypothetical protein -
  ANACY_RS21500 (Anacy_4349) - 4905457..4906680 (-) 1224 WP_015216327.1 type II secretion system F family protein -
  ANACY_RS21505 (Anacy_4350) pilT 4906838..4907962 (-) 1125 WP_015216328.1 type IV pilus twitching motility protein PilT Machinery gene
  ANACY_RS21510 (Anacy_4351) pilB/pilB1 4908071..4910083 (-) 2013 WP_015216329.1 GspE/PulE family protein Machinery gene
  ANACY_RS21515 (Anacy_4352) grpE 4910766..4911512 (+) 747 WP_015216330.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40921.19 Da        Isoelectric Point: 7.1626

>NTDB_id=55017 ANACY_RS21505 WP_015216328.1 4906838..4907962(-) (pilT) [Anabaena cylindrica PCC 7122]
MEMMIEDLMEQLIDMGGSDLHLSAGLPPYFRVSGHLTPIGDHILTADECQRLIFSMLNNTQRKTLEQTWELDCSYGVKGL
ARFRVNVYKERGAYAACLRALSSKIPNFEKLGLPDVVREMSEKPRGLILVTGPTGSGKTTTLAAMIDLINRTRAEHILTV
EDPVEFVYEPVKSLVHQRQLGEDTKSFANALKAALREDPDIILVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RMIDVFPHERQTQVRVQLSNSLVAVFSQTLVPKKNPKPGEYGRIMAQEIMIITPAISNLIREGKTAQIYSAIQTGGKLGM
QTLEKVLADLYKSGAISFEAAMSKTSKPDEVQRLIGTTAPAGAGAKPGTAARTR

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=55017 ANACY_RS21505 WP_015216328.1 4906838..4907962(-) (pilT) [Anabaena cylindrica PCC 7122]
ATGGAAATGATGATTGAAGACTTGATGGAGCAGCTAATTGATATGGGTGGCTCGGATCTGCACTTATCAGCAGGTTTGCC
TCCCTACTTCCGCGTCAGTGGTCATTTGACTCCCATTGGAGATCACATACTAACCGCAGATGAATGTCAAAGATTGATTT
TTAGTATGCTCAACAATACCCAGCGAAAAACCTTAGAACAAACCTGGGAATTGGATTGTTCCTATGGTGTCAAAGGATTA
GCTCGCTTCCGGGTGAATGTCTACAAAGAACGTGGTGCTTATGCAGCTTGTTTGCGAGCATTAAGTTCTAAAATTCCTAA
CTTTGAAAAATTAGGTCTACCAGATGTTGTCCGGGAAATGTCTGAAAAGCCCAGAGGATTAATTCTCGTCACCGGGCCGA
CAGGTTCCGGTAAAACTACTACTCTAGCCGCAATGATTGATCTCATTAATCGCACTAGAGCAGAACATATATTAACAGTA
GAAGACCCCGTAGAATTTGTTTACGAACCAGTAAAAAGCTTAGTTCACCAACGCCAACTAGGTGAAGATACTAAAAGCTT
TGCTAATGCCTTAAAAGCCGCCCTCCGGGAAGATCCAGATATTATTCTGGTAGGAGAAATGCGTGATTTGGAAACAATTT
CTTTGGCTATTTCCGCAGCGGAAACCGGACACTTAGTATTTGGAACGCTGCACACCAGTTCAGCCGCACAAACCGTTGAC
CGGATGATTGACGTTTTCCCTCATGAAAGACAAACCCAAGTGCGGGTACAGTTATCCAACTCTTTAGTAGCAGTTTTTAG
CCAAACTTTAGTACCCAAGAAAAATCCTAAGCCAGGTGAATATGGTCGAATCATGGCTCAAGAAATTATGATTATTACTC
CTGCTATTTCCAACCTAATTCGTGAAGGTAAAACTGCACAAATTTATTCAGCTATCCAAACTGGCGGTAAATTGGGAATG
CAAACTCTAGAAAAAGTCTTAGCTGATTTATACAAATCTGGAGCTATCTCCTTTGAAGCAGCTATGTCTAAAACTTCTAA
ACCCGATGAAGTTCAACGTCTAATTGGTACAACAGCACCTGCTGGTGCTGGTGCAAAACCTGGTACTGCTGCTAGAACTC
GTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(45-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

55.331

92.781

0.513
  pilT Pseudomonas stutzeri DSM 10701

54.467

92.781

0.505
  pilT Legionella pneumophila strain ERS1305867

53.602

92.781

0.497
  pilT Acinetobacter baylyi ADP1

53.602

92.781

0.497
  pilT Legionella pneumophila strain Lp02

53.602

92.781

0.497
  pilT Acinetobacter nosocomialis M2

53.314

92.781

0.495
  pilT Acinetobacter baumannii D1279779

53.314

92.781

0.495
  pilT Acinetobacter baumannii strain A118

53.314

92.781

0.495
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.684

98.128

0.468
  pilT Neisseria gonorrhoeae MS11

50.737

90.642

0.46
  pilT Neisseria meningitidis 8013

50.442

90.642

0.457
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.415

91.444

0.452
  pilT Vibrio cholerae strain A1552

49.415

91.444

0.452

Multiple sequence alignment