Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   LSI73_RS10250 Genome accession   NZ_CP089272
Coordinates   2058059..2059483 (-) Length   474 a.a.
NCBI ID   WP_000132546.1    Uniprot ID   -
Organism   Escherichia coli O157:H7 strain M1300706001A     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2053059..2064483
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSI73_RS10220 (LSI73_10220) yeeZ 2053114..2053938 (+) 825 WP_000754737.1 SDR family oxidoreductase -
  LSI73_RS10225 (LSI73_10225) yeeY 2053984..2054913 (+) 930 WP_000803334.1 LysR substrate-binding domain-containing protein -
  LSI73_RS10230 (LSI73_10230) yoeI 2055128..2055190 (+) 63 WP_010723108.1 membrane protein YoeI -
  LSI73_RS10235 (LSI73_10235) plaP 2055180..2056538 (+) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  LSI73_RS10240 (LSI73_10240) tsuA 2056717..2057775 (+) 1059 WP_000492327.1 thiosulfate utilization transporter TsuA/YeeE -
  LSI73_RS10245 (LSI73_10245) tsuB 2057789..2058016 (+) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  LSI73_RS10250 (LSI73_10250) sbcB 2058059..2059483 (-) 1425 WP_000132546.1 exodeoxyribonuclease I Machinery gene
  LSI73_RS10255 (LSI73_10255) - 2059665..2060843 (+) 1179 WP_001007946.1 tyrosine-type recombinase/integrase -
  LSI73_RS10260 (LSI73_10260) - 2060824..2061015 (-) 192 WP_000132739.1 helix-turn-helix transcriptional regulator -
  LSI73_RS10265 (LSI73_10265) - 2061093..2061437 (-) 345 WP_001281188.1 hypothetical protein -
  LSI73_RS10270 (LSI73_10270) - 2061625..2061975 (-) 351 WP_000610373.1 DUF551 domain-containing protein -
  LSI73_RS10275 (LSI73_10275) - 2061972..2062328 (-) 357 WP_000207903.1 hypothetical protein -
  LSI73_RS10280 (LSI73_10280) - 2062662..2062838 (-) 177 WP_001356547.1 hypothetical protein -
  LSI73_RS10285 (LSI73_10285) - 2062840..2063787 (-) 948 WP_001289930.1 ead/Ea22-like family protein -
  LSI73_RS10290 (LSI73_10290) - 2063784..2064005 (-) 222 WP_000763383.1 TraR/DksA family transcriptional regulator -
  LSI73_RS10295 (LSI73_10295) - 2064104..2064319 (-) 216 WP_000188870.1 hypothetical protein -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 54397.63 Da        Isoelectric Point: 5.2440

>NTDB_id=549967 LSI73_RS10250 WP_000132546.1 2058059..2059483(-) (sbcB) [Escherichia coli O157:H7 strain M1300706001A]
MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENE
AAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWRG
NTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDAD
RLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVDK
RIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=549967 LSI73_RS10250 WP_000132546.1 2058059..2059483(-) (sbcB) [Escherichia coli O157:H7 strain M1300706001A]
ATGACAGACACAGATAAGCAACCTACCTTCCTCTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCC
TGCACAGTTCGCAGCCATTCGCACCGATGACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCGG
ATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCTCGGGCGAAAGGAGAAAACGAA
GCCGCATTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGA
CGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCT
GGGATTTACTGGATGTTATGCGTGCCTGCTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGTCTA
CCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGTGTA
CGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAACCACGCCTGTTTGATTATCTCTTTACCCATCGTAATAAAC
ACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGCGGC
AATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGATAT
TTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCG
CCGTTCCGGTTAAACTGGTACATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGAC
CGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGTGGT
GGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAATGGCTTTTTCAGTGACG
CCGATCGTGCAGCAATGAAAATTGTGCTTGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGATAAA
CGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCT
GGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTACAAGGCTATGCAGAAGAGATTCAGATGCTGGCGCAGCAGTATG
CCGACGATAAAGAAAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATTGTCTAA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(12-187)

Exonuclease C-terminal (ExoI_SH3)

(211-471)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.936

99.156

0.584