Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CRI9333_RS22780 Genome accession   NC_019753
Coordinates   5249426..5250532 (-) Length   368 a.a.
NCBI ID   WP_015205516.1    Uniprot ID   K9W5E9
Organism   Crinalium epipsammum PCC 9333     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5244426..5255532
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CRI9333_RS22760 (Cri9333_4642) - 5244696..5244980 (-) 285 WP_015205512.1 HU family DNA-binding protein -
  CRI9333_RS22765 (Cri9333_4643) - 5245507..5247540 (-) 2034 WP_041226165.1 secretin and TonB N-terminal domain-containing protein -
  CRI9333_RS22770 (Cri9333_4644) - 5247776..5248531 (-) 756 WP_015205514.1 hypothetical protein -
  CRI9333_RS22775 (Cri9333_4645) - 5248528..5249421 (-) 894 WP_015205515.1 PilN domain-containing protein -
  CRI9333_RS22780 (Cri9333_4646) pilM 5249426..5250532 (-) 1107 WP_015205516.1 type IV pilus assembly protein PilM Machinery gene
  CRI9333_RS22785 (Cri9333_4647) - 5250816..5252087 (+) 1272 WP_015205517.1 sugar ABC transporter substrate-binding protein -
  CRI9333_RS22790 (Cri9333_4648) - 5252188..5253453 (-) 1266 WP_015205518.1 folylpolyglutamate synthase/dihydrofolate synthase family protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40461.48 Da        Isoelectric Point: 4.3979

>NTDB_id=54971 CRI9333_RS22780 WP_015205516.1 5249426..5250532(-) (pilM) [Crinalium epipsammum PCC 9333]
MFNRLKSFIPRRGQGIGIELAPERINIAQVRKQGQGFKLTMLSSVEIPEGLIQEGKITDSPGLAELIQSTLAESKIKVDR
VATAVPMREAVIRIIPVPAELNDKELREMVLDHEAGLYLPYPREEVDVDYQKLDLIVDEDGIEKVQVLLVATKKEITDSY
LETFQQAGLQVDVLEINSFALIRTIREQLRQFGSQEAAVLVDIEFDSTEIAIIVNGVPQFNRTVPIGTYQLQNALSRAMN
LPTSRNTDLLQGMTIPTNSPVDSVRTGATGINPGMAALLRVLGELTDELRRSIDFYLNQSENLEVAQLLLAGPGAGMGQL
DEFFTQRLSVPTTQIDPVAALGLQVDQEIPALQRPGLGTVLGLGLREV

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=54971 CRI9333_RS22780 WP_015205516.1 5249426..5250532(-) (pilM) [Crinalium epipsammum PCC 9333]
ATGTTTAACCGTTTAAAAAGTTTCATCCCCAGAAGAGGTCAAGGAATTGGTATAGAACTTGCCCCCGAACGGATTAATAT
TGCTCAAGTCCGTAAGCAAGGTCAAGGATTTAAACTAACCATGTTGTCCTCAGTAGAAATACCTGAAGGGCTAATTCAAG
AAGGCAAGATAACAGACTCACCAGGCTTGGCAGAACTAATTCAGTCAACATTGGCAGAGAGTAAGATTAAAGTAGACCGA
GTAGCTACTGCCGTTCCGATGCGGGAAGCAGTGATCAGGATCATCCCAGTTCCAGCAGAATTGAACGATAAAGAATTAAG
GGAGATGGTACTCGACCATGAAGCTGGTCTTTATTTGCCTTATCCTAGAGAAGAAGTTGATGTCGATTATCAGAAACTGG
ATTTAATTGTGGATGAAGACGGCATTGAAAAAGTGCAAGTTTTGTTGGTAGCCACCAAAAAAGAAATTACTGATAGTTAT
CTAGAAACATTTCAGCAAGCTGGCTTACAGGTAGATGTATTAGAAATCAACAGCTTTGCTCTAATTAGGACAATTAGAGA
GCAGTTGCGGCAATTCGGTTCTCAAGAAGCCGCCGTCTTGGTAGATATTGAGTTTGATAGCACTGAAATTGCCATTATCG
TCAACGGCGTTCCACAATTTAATCGCACTGTTCCTATTGGTACTTACCAACTCCAGAACGCCCTCAGCCGCGCCATGAAC
CTACCAACGTCAAGAAATACCGATCTTTTACAAGGAATGACGATTCCGACTAATTCCCCTGTTGATAGTGTACGCACTGG
CGCAACTGGGATAAATCCTGGTATGGCTGCTCTTTTAAGAGTTTTAGGAGAACTAACTGATGAATTACGTCGCTCTATAG
ATTTTTATTTGAATCAAAGCGAAAATTTGGAAGTCGCGCAGTTGTTGTTGGCAGGGCCAGGAGCAGGTATGGGACAACTT
GACGAATTCTTTACACAAAGGTTAAGCGTGCCTACTACTCAGATAGACCCAGTAGCAGCCCTTGGTTTACAGGTAGATCA
AGAAATTCCTGCACTCCAGAGACCAGGCTTAGGAACGGTATTGGGATTAGGACTGCGGGAGGTGTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(284-365)

Type IV pilus assembly protein PilM; (PilM_2)

(17-242)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9W5E9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

70.572

99.728

0.704

Multiple sequence alignment