Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   J5N61_RS12210 Genome accession   NZ_CP071973
Coordinates   2583818..2584936 (+) Length   372 a.a.
NCBI ID   WP_004913902.1    Uniprot ID   S7Y7Y7
Organism   Acinetobacter junii strain INC8271     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2578818..2589936
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5N61_RS12200 (J5N61_12200) - 2581941..2582627 (-) 687 WP_004913896.1 YggS family pyridoxal phosphate-dependent enzyme -
  J5N61_RS12205 (J5N61_12205) pilT 2582755..2583792 (+) 1038 WP_004913899.1 type IV pilus twitching motility protein PilT Machinery gene
  J5N61_RS12210 (J5N61_12210) pilU 2583818..2584936 (+) 1119 WP_004913902.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J5N61_RS12215 (J5N61_12215) fur 2585033..2585470 (-) 438 WP_004913905.1 ferric iron uptake transcriptional regulator -
  J5N61_RS12220 (J5N61_12220) - 2585582..2585980 (+) 399 WP_004913908.1 outer membrane protein assembly factor BamE -
  J5N61_RS12225 (J5N61_12225) - 2586036..2586359 (-) 324 WP_004913910.1 RnfH family protein -
  J5N61_RS12230 (J5N61_12230) - 2586359..2587438 (-) 1080 WP_125272216.1 hypothetical protein -
  J5N61_RS12235 (J5N61_12235) - 2587677..2588132 (-) 456 WP_004913914.1 bacteriohemerythrin -
  J5N61_RS12240 (J5N61_12240) - 2588415..2589263 (-) 849 WP_005403076.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41716.88 Da        Isoelectric Point: 6.1368

>NTDB_id=549556 J5N61_RS12210 WP_004913902.1 2583818..2584936(+) (pilU) [Acinetobacter junii strain INC8271]
MDFNDLLNLMIEQSASDLFVTADVEPSMKINGQIVPVAKAKLSGEIVGQLLHSIMSDKQRKEFEDTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETVIPTMDELKLPAILKDLAMTKRGIIIFVGATGTGKSTSLASMIGYRNQNSKGHIITIE
DPIEFVHQHAGCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVLATLHANNANQALDR
IIHFFEADRHAQLHMDLSLNLRAMVAQQLIPTPDGNSRRAAIEILINTPLLSDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALYDLYKAGEITYKDALKHADSPNDLRLTIKLSEEGADQLLSASRNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=549556 J5N61_RS12210 WP_004913902.1 2583818..2584936(+) (pilU) [Acinetobacter junii strain INC8271]
ATGGATTTTAATGATTTACTCAATCTAATGATTGAACAAAGTGCTTCGGATTTATTTGTTACCGCTGATGTTGAACCTTC
AATGAAAATCAACGGTCAGATTGTTCCTGTGGCTAAAGCAAAACTTTCAGGTGAGATTGTAGGTCAGTTATTACACTCAA
TCATGAGTGATAAACAACGTAAAGAATTCGAAGATACTCGCGAGTGTAACTTCGCAATTATGAACCGTGAGAAAACAGCG
CGTTTTCGTGTGAGCGCGTTCCAACAACGTGATATGCCAGGCATGGTATTACGTCGTATCGAAACGGTTATTCCAACAAT
GGATGAATTAAAACTACCTGCAATCCTCAAAGATTTAGCGATGACCAAACGTGGCATCATTATCTTTGTTGGTGCAACAG
GTACAGGTAAATCAACCTCGCTCGCGTCAATGATCGGATATCGTAATCAAAACTCTAAAGGTCATATCATCACCATTGAA
GACCCGATCGAGTTTGTACACCAACACGCGGGTTGTATTGTGACTCAACGCGAAGTCGGTATTGATACAGACTCTTTTGA
AGTAGCTTTGAAAAATACACTTCGTCAAGCACCCGATGTGATCTTGATTGGTGAGATTCGCTCTCGTGAAACAATGGACT
ATGCGATTGCTTTCGCAGAAACAGGTCACTTAGTACTTGCTACACTTCACGCGAATAACGCTAACCAAGCATTAGACCGT
ATTATTCACTTCTTTGAAGCTGACCGTCATGCTCAACTACATATGGATTTATCGCTCAATCTAAGAGCAATGGTCGCTCA
ACAGTTAATTCCAACACCTGATGGAAATTCACGCCGAGCAGCTATCGAAATTCTAATTAATACACCGTTACTATCAGATT
ATATCCGTAAAGGTGAAATTCATGAGATTAAAGACCTCATGAAACGCTCGCGCGAATTGGGCATGCAAACATTCGACCAA
GCGCTTTATGATTTATATAAAGCTGGTGAAATCACGTATAAAGATGCGCTCAAACATGCAGACTCACCAAACGATTTACG
TCTCACCATTAAACTTTCTGAAGAAGGTGCAGATCAATTGTTAAGTGCAAGTCGCAACATTACATTTGATGGTCAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.132

100

0.763
  pilU Pseudomonas stutzeri DSM 10701

70.62

99.731

0.704
  pilU Vibrio cholerae strain A1552

55.135

99.462

0.548
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395
  pilT Pseudomonas aeruginosa PAK

39.884

93.011

0.371
  pilT Neisseria gonorrhoeae MS11

40.058

91.935

0.368
  pilT Neisseria meningitidis 8013

40.058

91.935

0.368
  pilT Acinetobacter nosocomialis M2

39.65

92.204

0.366
  pilT Acinetobacter baumannii D1279779

39.65

92.204

0.366
  pilT Acinetobacter baumannii strain A118

39.65

92.204

0.366
  pilT Legionella pneumophila strain Lp02

40.476

90.323

0.366
  pilT Legionella pneumophila strain ERS1305867

40.476

90.323

0.366