Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   J5N61_RS02910 Genome accession   NZ_CP071973
Coordinates   616413..617213 (+) Length   266 a.a.
NCBI ID   WP_004908991.1    Uniprot ID   -
Organism   Acinetobacter junii strain INC8271     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 611413..622213
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5N61_RS02885 (J5N61_02885) - 612322..613638 (+) 1317 WP_171191619.1 glycosyltransferase family 4 protein -
  J5N61_RS02890 (J5N61_02890) - 613666..614217 (+) 552 WP_004908998.1 phosphatase PAP2 family protein -
  J5N61_RS02895 (J5N61_02895) iscX 614300..614500 (+) 201 WP_004652532.1 Fe-S cluster assembly protein IscX -
  J5N61_RS02900 (J5N61_02900) ndk 614608..615039 (+) 432 WP_004908995.1 nucleoside-diphosphate kinase -
  J5N61_RS02905 (J5N61_02905) rlmN 615161..616396 (+) 1236 WP_004950309.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  J5N61_RS02910 (J5N61_02910) pilF 616413..617213 (+) 801 WP_004908991.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  J5N61_RS02915 (J5N61_02915) - 617204..617995 (+) 792 WP_004964120.1 helix-turn-helix domain-containing protein -
  J5N61_RS02920 (J5N61_02920) ispG 618020..619135 (+) 1116 WP_004908985.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  J5N61_RS02925 (J5N61_02925) hisS 619132..620424 (+) 1293 WP_212388100.1 histidine--tRNA ligase -
  J5N61_RS02930 (J5N61_02930) - 620456..621154 (+) 699 WP_004950316.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30366.44 Da        Isoelectric Point: 9.5600

>NTDB_id=549546 J5N61_RS02910 WP_004908991.1 616413..617213(+) (pilF) [Acinetobacter junii strain INC8271]
MNTTRLKTILATTVVFTVLGLTACQSTTLRKDPEKAVQVRTQLAAEYIRTRDLDSAKRSLDQALKIDPRDATANMMMGVL
LQQEGSKPNMEKAEAYFKRAISSEPDNAQARNNYGTYLYQMQRYNEAIEQFTVAGATLGYEQRYQSLENLGRIYLKLGDV
ANAEKSFKQALQANRNSTISMLELSEIFYLQQNNRDASQLYEQYVRNVGQKNQGARALWIGIRIARANADQLGMQVLVNQ
MRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=549546 J5N61_RS02910 WP_004908991.1 616413..617213(+) (pilF) [Acinetobacter junii strain INC8271]
TTGAATACAACTCGACTAAAAACGATTTTAGCTACTACTGTTGTGTTTACTGTATTAGGTTTGACTGCCTGCCAATCAAC
AACGTTAAGAAAAGATCCAGAAAAGGCAGTACAAGTCCGAACTCAACTCGCTGCTGAATATATTCGAACACGTGATTTAG
ATTCAGCGAAACGCTCCCTTGATCAAGCTTTAAAAATTGATCCACGTGATGCTACTGCCAATATGATGATGGGTGTTTTG
TTGCAACAAGAAGGCAGCAAACCAAACATGGAGAAAGCAGAAGCTTATTTTAAGCGAGCAATCTCTTCAGAACCCGATAA
TGCCCAAGCTCGTAATAATTACGGTACATATTTGTATCAAATGCAACGTTATAATGAGGCGATTGAGCAATTTACCGTTG
CAGGTGCGACGTTAGGTTACGAACAGCGTTACCAATCGTTGGAAAACTTGGGTCGAATCTATTTAAAACTGGGTGATGTT
GCGAATGCTGAAAAATCATTCAAGCAAGCCTTACAAGCAAATCGAAATTCGACAATTTCAATGTTAGAGTTGTCCGAAAT
TTTCTATTTGCAACAAAATAATAGAGATGCATCGCAACTTTATGAACAATACGTTCGTAATGTCGGGCAGAAAAATCAAG
GTGCTCGTGCACTTTGGATCGGTATACGCATTGCTCGAGCGAATGCGGATCAATTAGGAATGCAAGTATTAGTGAATCAA
ATGCGTGCTTTATTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-69)

Tetratricopeptide repeat (TPR_8)

(145-176)

Tetratricopeptide repeat (TPR_16)

(90-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.026

100

0.793
  pilF Acinetobacter baylyi ADP1

68.045

100

0.68