Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   J5F47_RS14540 Genome accession   NZ_CP071931
Coordinates   2685634..2687004 (-) Length   456 a.a.
NCBI ID   WP_002303449.1    Uniprot ID   A0A829F2L9
Organism   Enterococcus faecium strain VRE3355     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2680634..2692004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5F47_RS14525 (J5F47_14535) epsC 2681955..2682491 (-) 537 WP_002287446.1 serine O-acetyltransferase EpsC -
  J5F47_RS14530 (J5F47_14540) gltX 2682813..2684273 (-) 1461 WP_002287447.1 glutamate--tRNA ligase -
  J5F47_RS14535 (J5F47_14545) - 2684443..2685600 (-) 1158 WP_002287448.1 PIN/TRAM domain-containing protein -
  J5F47_RS14540 (J5F47_14550) radA 2685634..2687004 (-) 1371 WP_002303449.1 DNA repair protein RadA Machinery gene
  J5F47_RS14545 (J5F47_14555) - 2687067..2687594 (-) 528 WP_002287450.1 dUTP diphosphatase -
  J5F47_RS14550 (J5F47_14560) proB 2687824..2688630 (+) 807 WP_002287451.1 glutamate 5-kinase -
  J5F47_RS14555 (J5F47_14565) - 2688627..2689874 (+) 1248 WP_002298218.1 glutamate-5-semialdehyde dehydrogenase -
  J5F47_RS14560 (J5F47_14570) - 2689950..2690294 (+) 345 WP_002287507.1 hypothetical protein -
  J5F47_RS14565 (J5F47_14575) - 2690411..2690737 (-) 327 WP_002287453.1 hypothetical protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49937.58 Da        Isoelectric Point: 7.7416

>NTDB_id=549194 J5F47_RS14540 WP_002303449.1 2685634..2687004(-) (radA) [Enterococcus faecium strain VRE3355]
MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTDRRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEVANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECFIGEIGLTG
EIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAETLKRVFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=549194 J5F47_RS14540 WP_002303449.1 2685634..2687004(-) (radA) [Enterococcus faecium strain VRE3355]
ATGGCAAAAAAATCAAAAGTACAATTTGTTTGCCAAAATTGCGGGTATGTGTCGCCTAAGTTCTTGGGACGCTGCCCAAA
TTGCGGAAAATGGAATACGATGGTCGAAGAAATCGAACAAGATACAACGGACCGCAGAACTCGGACAAGTTTGACAGGAG
AAAAAGCAAAACCAACAAAAATCGCAGACGTCGTCCCTAAAAAAGAACCTCGGATCAAAACAAAATTAGAAGAACTGAAC
CGTGTGTTAGGCGGAGGTGTGGTGCCAGGGTCTATGGTCTTGATTGGTGGCGATCCAGGTATCGGGAAATCCACTTTGCT
GTTACAAGTTTCTCAGCAATTAGCAGCCATAGGCGGAAAAGTCTTATATGTATCCGGCGAAGAAAGCGCAGAGCAGATAA
AATTACGCGCGGAACGCCTCGGAAGTATCAACACAGAATTTTATCTATACGCTGAAACAGATATGAACGAAATCAGTCGT
GCCATCGAACATATCTCCCCTGATTATGTTATCATTGATTCCATCCAAACGATGACGCAGCCAGACATCACAAGTGTGGC
AGGAAGTGTCAGCCAAGTAAGAGAAACCACTGCAGAACTACTCAAAACCGCTAAAACAAATGGGATAGCCATTTTTATCG
TCGGTCATGTTACCAAAGAAGGCTCGATCGCCGGCCCAAGAATGTTAGAACATATGGTGGATACGGTGTTATATTTTGAA
GGTGAACAACATCATAGTTTTCGGATTTTGCGAGCAGTAAAAAACCGATTTGGCTCAACGAATGAGATTGGGATTTTTGA
AATGCATGAGCATGGATTGGAAGAAGTCGCTAATCCTTCACAGATTTTCTTAGAAGAACGCCTAGACGGTGCAACTGGCT
CTGCTATCGTTGTGGCAATGGAAGGGACACGTCCAATTTTGGTAGAGATCCAAGCATTAGTCACACCTACTATGTTCGGA
AATGCCAAACGAACAACAACAGGTCTTGATTTCAATCGGGTATCTCTGATCATGGCAGTGCTGGAAAAAAGAGCTGGGTT
GCTTCTTCAGAATCAAGATGCTTACTTAAAAGCTGCAGGTGGGGTAAAATTAAATGAACCAGCGATTGATTTAGCTATCG
CTGTCAGTATTGCTTCAAGCTATAAAGAAAAAGGAACACAGCCAACGGAATGTTTCATCGGTGAAATTGGACTGACTGGA
GAAATCAGACGTGTCAGTCATATTGAACAACGAGTCAAAGAAGTCCAAAAACTCGGCTTCACAAAAGTATACCTACCTAA
AAACAACTTAGGTAACTGGGAAGCGCCAAAAGGAATCGAGATAGTTGGAGTAGCAACTTTGGCTGAAACGTTGAAGCGTG
TGTTCCGCTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

AAA domain (AAA_25)

(69-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A829F2L9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.286

99.781

0.741
  radA Streptococcus pneumoniae Rx1

74.286

99.781

0.741
  radA Streptococcus pneumoniae D39

74.286

99.781

0.741
  radA Streptococcus pneumoniae R6

74.286

99.781

0.741
  radA Streptococcus pneumoniae TIGR4

74.286

99.781

0.741
  radA Streptococcus mitis SK321

74.066

99.781

0.739
  radA Bacillus subtilis subsp. subtilis str. 168

67.184

98.904

0.664