Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GLO7428_RS13585 Genome accession   NC_019745
Coordinates   3088120..3089250 (-) Length   376 a.a.
NCBI ID   WP_015189129.1    Uniprot ID   K9XHW6
Organism   Gloeocapsa sp. PCC 7428     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3083120..3094250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GLO7428_RS29050 (Glo7428_2753) - 3083625..3085967 (-) 2343 WP_015189126.1 type IV pilus secretin family protein -
  GLO7428_RS13575 (Glo7428_2754) - 3086454..3087206 (-) 753 WP_015189127.1 hypothetical protein -
  GLO7428_RS13580 (Glo7428_2755) - 3087203..3088075 (-) 873 WP_015189128.1 PilN domain-containing protein -
  GLO7428_RS13585 (Glo7428_2756) pilM 3088120..3089250 (-) 1131 WP_015189129.1 type IV pilus assembly protein PilM Machinery gene
  GLO7428_RS13590 (Glo7428_2757) - 3089517..3090839 (+) 1323 WP_015189130.1 ABC transporter substrate-binding protein -
  GLO7428_RS13595 (Glo7428_2758) - 3090934..3091476 (-) 543 WP_015189131.1 single-stranded DNA-binding protein -
  GLO7428_RS13600 (Glo7428_2759) - 3091642..3094200 (-) 2559 WP_015189132.1 mannose-1-phosphate guanyltransferase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41553.00 Da        Isoelectric Point: 4.5799

>NTDB_id=54914 GLO7428_RS13585 WP_015189129.1 3088120..3089250(-) (pilM) [Gloeocapsa sp. PCC 7428]
MLNRFQTLPNRLQILPKRTKGVGVELAPERINIAQLRKQGQGIRLAAFTSVPVPEGIFQDGQIIDPPALAEIIRSALAQS
KIKAKRVATAVPGRDSLVRLIPLPAELDDQELREMVLNHEAGLYLPYPREEADVDYQKLGYFVDEDGIEKVQVLLVATRK
EVTDTYIDTFQQAGLQVDVLEINSFALLRTIREKLREFPSQEAVVLVDIEFDNTEIAIIVDGIPQFSRTVPIGTFHLQSA
LLRALHLPMSRDTQVLLQSVIIPTTTTDEMGGVISTTVDPGTAAMLRVMGELIDELRRSLDFYLSQSENLEVAQILLAGP
GGGLVNLDEFFQQRVNIPTVQIDPVAALSLEVDEKKITPGQRPGLGIVLGLGMREI

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=54914 GLO7428_RS13585 WP_015189129.1 3088120..3089250(-) (pilM) [Gloeocapsa sp. PCC 7428]
GTGCTTAACCGCTTCCAGACACTACCTAACCGCTTACAAATACTGCCAAAACGAACTAAAGGAGTTGGCGTTGAACTGGC
TCCTGAACGGATTAATATTGCCCAGCTACGCAAACAAGGACAGGGAATAAGACTGGCAGCGTTTACCTCGGTTCCCGTAC
CCGAAGGAATTTTTCAGGATGGTCAAATTATTGATCCACCAGCGCTTGCTGAAATTATTCGATCCGCCTTAGCCCAAAGC
AAAATAAAAGCTAAGCGAGTTGCTACCGCTGTTCCTGGAAGAGATTCACTCGTGCGTTTAATTCCTTTACCTGCCGAGTT
AGATGACCAAGAATTACGCGAAATGGTACTCAATCACGAAGCGGGTTTGTATCTCCCGTATCCGCGTGAAGAAGCTGATG
TCGATTATCAAAAACTTGGGTATTTTGTGGATGAGGACGGAATTGAAAAGGTACAAGTCTTGTTAGTTGCTACGCGCAAA
GAAGTTACGGATACTTACATCGATACATTTCAACAAGCAGGGTTACAAGTTGATGTTTTAGAAATTAATAGTTTTGCGTT
GTTACGCACGATTCGGGAGAAACTGCGCGAATTTCCTTCGCAAGAAGCCGTTGTTTTGGTTGATATTGAATTTGACAACA
CTGAAATTGCCATTATTGTAGATGGAATACCGCAGTTTTCGCGGACTGTCCCGATTGGTACATTTCATCTGCAAAGTGCG
CTGTTGCGAGCACTGCACTTACCAATGTCTAGAGATACACAAGTCTTACTACAGTCGGTGATTATTCCCACCACGACAAC
TGATGAAATGGGAGGAGTCATCAGTACGACAGTTGACCCTGGTACCGCGGCAATGTTGCGCGTGATGGGAGAGTTGATTG
ACGAATTACGGCGATCGCTCGATTTTTATCTCAGTCAAAGTGAAAATCTCGAAGTTGCCCAAATTCTCTTGGCAGGACCT
GGTGGCGGATTAGTAAACTTAGATGAGTTTTTCCAACAACGAGTAAACATTCCCACCGTACAAATCGATCCTGTGGCAGC
TTTGTCATTAGAAGTTGATGAGAAAAAAATTACTCCTGGACAACGCCCAGGACTCGGAATTGTTCTTGGTTTAGGAATGC
GCGAGATTTAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(291-375)

Type IV pilus assembly protein PilM; (PilM_2)

(23-241)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9XHW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

63.2

99.734

0.63

Multiple sequence alignment