Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LRO88_RS09070 Genome accession   NZ_CP089148
Coordinates   1714051..1715040 (+) Length   329 a.a.
NCBI ID   WP_003232026.1    Uniprot ID   P51835
Organism   Bacillus subtilis strain NIB353     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1709051..1720040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRO88_RS09055 acpP 1709248..1709481 (+) 234 WP_003154310.1 acyl carrier protein -
  LRO88_RS09060 rncS 1709621..1710370 (+) 750 WP_003232030.1 ribonuclease III -
  LRO88_RS09065 smc 1710471..1714031 (+) 3561 WP_231834260.1 chromosome segregation protein SMC -
  LRO88_RS09070 pilA 1714051..1715040 (+) 990 WP_003232026.1 signal recognition particle-docking protein FtsY Machinery gene
  LRO88_RS09075 sivC 1715077..1715562 (-) 486 WP_003245834.1 Ig-like domain-containing protein -
  LRO88_RS09080 srpM 1715739..1716071 (+) 333 WP_021479302.1 putative DNA-binding protein -
  LRO88_RS09085 ffh 1716085..1717425 (+) 1341 WP_014113771.1 signal recognition particle protein -
  LRO88_RS09090 rpsP 1717531..1717803 (+) 273 WP_003220815.1 30S ribosomal protein S16 -
  LRO88_RS09095 khpA 1717803..1718048 (+) 246 WP_014476792.1 KH domain-containing protein -
  LRO88_RS09100 ylqD 1718170..1718556 (+) 387 WP_015715875.1 YlqD family protein -
  LRO88_RS09105 rimM 1718561..1719085 (+) 525 WP_003232013.1 ribosome maturation factor RimM -
  LRO88_RS09110 trmD 1719082..1719813 (+) 732 WP_071580705.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -

Sequence

Protein


Download         Length: 329 a.a.        Molecular weight: 36342.52 Da        Isoelectric Point: 5.0164

>NTDB_id=548956 LRO88_RS09070 WP_003232026.1 1714051..1715040(+) (pilA) [Bacillus subtilis strain NIB353]
MSFFKKLKEKITKQTDSVSEKFKDGLEKTRNSFQNKVNDLVSRYRKVDEDFFEELEEVLISADVGFTTVMELIDELKKEV
KRRNIQDPKEVQSVISEKLVEIYNSGDEQISELNIQDGRLNVILLVGVNGVGKTTTIGKLAHKMKQEGKSVVLAAGDTFR
AGAIEQLEVWGERTGVPVIKQTAGSDPAAVIYDAVHAAKARNADVLICDTAGRLQNKVNLMKELEKVKRVIEREVPEAPH
EVLLALDATTGQNAMAQAKEFSKATNVTGIALTKLDGTAKGGIVLAIRNELHIPVKLVGLGEKVDDLQEFDPESYVYGLF
SDLVEKADD

Nucleotide


Download         Length: 990 bp        

>NTDB_id=548956 LRO88_RS09070 WP_003232026.1 1714051..1715040(+) (pilA) [Bacillus subtilis strain NIB353]
ATGAGCTTTTTTAAAAAATTAAAAGAGAAAATCACAAAACAGACAGATTCCGTATCTGAAAAGTTTAAGGATGGCCTTGA
AAAAACAAGAAACTCCTTTCAAAACAAAGTGAATGATCTTGTATCCCGTTACCGTAAAGTGGATGAGGATTTCTTCGAAG
AGCTTGAAGAGGTTCTTATCAGCGCGGATGTCGGTTTTACAACCGTTATGGAATTAATAGATGAGCTGAAAAAAGAAGTC
AAACGCAGAAATATTCAAGATCCAAAGGAAGTCCAGTCAGTGATTTCTGAGAAACTGGTCGAGATTTATAACAGCGGAGA
TGAGCAAATTTCAGAACTGAACATCCAGGATGGGCGTTTAAACGTAATCCTTCTGGTAGGTGTAAACGGCGTCGGGAAAA
CAACAACGATCGGAAAGCTTGCTCATAAAATGAAACAAGAAGGAAAATCTGTCGTGCTTGCTGCCGGAGACACTTTCAGA
GCGGGAGCCATTGAACAGCTGGAAGTATGGGGAGAGCGTACAGGAGTGCCTGTCATTAAGCAGACGGCCGGAAGCGATCC
GGCAGCTGTCATCTACGATGCTGTTCATGCTGCGAAAGCAAGAAATGCCGATGTATTAATTTGTGATACGGCAGGGCGTC
TCCAAAACAAAGTAAATCTCATGAAAGAGCTTGAAAAAGTAAAACGTGTTATCGAAAGAGAAGTTCCTGAAGCTCCGCAT
GAGGTGCTGCTTGCCCTTGATGCCACGACCGGCCAAAATGCAATGGCTCAGGCAAAAGAATTCTCTAAAGCAACAAATGT
TACCGGCATTGCTTTAACGAAGCTTGACGGTACGGCAAAGGGCGGTATCGTCCTTGCGATTCGCAACGAGCTTCACATCC
CGGTTAAACTAGTCGGTTTAGGAGAAAAAGTTGATGATCTTCAGGAATTTGATCCAGAATCCTATGTGTACGGACTCTTT
TCAGATTTAGTGGAAAAAGCCGACGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(121-320)

SRP54-type protein, helical bundle domain (SRP54_N)

(34-99)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P51835

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

43.709

91.793

0.401