Detailed information NTG0548813

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: K0768_RS04220
• Genome accession: NZ_CP089142
• Coordinates: 177224..178207 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain XD35
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 172224..183207

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K0768_RS04205 (LS330_04205)	dppA	173367..174974 (+)	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-
K0768_RS04210 (LS330_04210)	dppB	175282..176301 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
K0768_RS04215 (LS330_04215)	dppC	176311..177213 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
K0768_RS04220 (LS330_04220)	amiE	177224..178207 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
K0768_RS04225 (LS330_04225)	dppF	178204..179208 (+)	1005	WP_000107012.1	dipeptide ABC transporter ATP-binding subunit DppF	-
K0768_RS04230 (LS330_04230)	yhjV	179238..180509 (-)	1272	WP_001295225.1	aromatic amino acid transport family protein	-
K0768_RS04235 (LS330_04235)	ldrD	180985..181092 (+)	108	WP_000141633.1	type I toxin-antitoxin system toxin Ldr family protein	-
K0768_RS04240 (LS330_04240)	bcsG	181179..182858 (-)	1680	WP_063084555.1	cellulose biosynthesis protein BcsG	-
K0768_RS04245 (LS330_04245)	bcsF	182855..183046 (-)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=548813 K0768_RS04220 WP_001196486.1 177224..178207(+) (amiE) [Escherichia coli strain XD35]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=548813 K0768_RS04220 WP_001196486.1 177224..178207(+) (amiE) [Escherichia coli strain XD35]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCGACCACCGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACCGGTGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404