Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   LSP20_RS06170 Genome accession   NZ_CP089065
Coordinates   1316780..1317376 (+) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain UNC_PaerCF34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1311780..1322376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSP20_RS06160 (LSP20_06160) - 1313060..1315531 (-) 2472 WP_023518163.1 penicillin-binding protein 1A -
  LSP20_RS06165 (LSP20_06165) pilM 1315716..1316780 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  LSP20_RS06170 (LSP20_06170) pilN 1316780..1317376 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  LSP20_RS06175 (LSP20_06175) pilO 1317373..1317996 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  LSP20_RS06180 (LSP20_06180) comL/pilP 1317993..1318517 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  LSP20_RS06185 (LSP20_06185) pilQ 1318571..1320715 (+) 2145 WP_015503770.1 type 4a pilus secretin PilQ Machinery gene
  LSP20_RS06190 (LSP20_06190) aroK 1320727..1321245 (+) 519 WP_003095833.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=548540 LSP20_RS06170 WP_003095839.1 1316780..1317376(+) (pilN) [Pseudomonas aeruginosa strain UNC_PaerCF34]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=548540 LSP20_RS06170 WP_003095839.1 1316780..1317376(+) (pilN) [Pseudomonas aeruginosa strain UNC_PaerCF34]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCTGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGGCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATCGCCATCGCCGGCGCGGCCGAGTCCA
ATAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGCTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369