Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LSP18_RS23435 Genome accession   NZ_CP089063
Coordinates   5013294..5013746 (-) Length   150 a.a.
NCBI ID   WP_024947914.1    Uniprot ID   A4GRK7
Organism   Pseudomonas aeruginosa strain UNC_PaerCF37     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5008294..5018746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSP18_RS23415 (LSP18_23635) ampD 5009000..5009566 (-) 567 WP_003112844.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LSP18_RS23420 (LSP18_23640) - 5009717..5011972 (-) 2256 WP_009315900.1 DUF1631 domain-containing protein -
  LSP18_RS23425 (LSP18_23645) nadC 5012258..5013106 (+) 849 WP_003125200.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LSP18_RS23435 (LSP18_23655) pilA 5013294..5013746 (-) 453 WP_024947914.1 pilin Machinery gene
  LSP18_RS23440 (LSP18_23660) pilB 5013975..5015675 (+) 1701 WP_009875880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LSP18_RS23445 (LSP18_23665) pilC 5015679..5016899 (+) 1221 WP_016253888.1 type 4a pilus biogenesis protein PilC Machinery gene
  LSP18_RS23450 (LSP18_23670) pilD 5016903..5017775 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  LSP18_RS23455 (LSP18_23675) coaE 5017772..5018383 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  LSP18_RS23460 (LSP18_23680) yacG 5018380..5018580 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15622.02 Da        Isoelectric Point: 8.4827

>NTDB_id=548366 LSP18_RS23435 WP_024947914.1 5013294..5013746(-) (pilA) [Pseudomonas aeruginosa strain UNC_PaerCF37]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALASVNPLKTTVEEALSRGWSVKSGTGTEDATKKEVPLG
VAADANKLGTIALKPDPADGTADITLTFTMGGAGPKNKGKIITLTRTAADGLWKCTSDQDEQFIPKGCSK

Nucleotide


Download         Length: 453 bp        

>NTDB_id=548366 LSP18_RS23435 WP_024947914.1 5013294..5013746(-) (pilA) [Pseudomonas aeruginosa strain UNC_PaerCF37]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCTTGGCTGCAATTGCCAT
TCCTCAGTATCAGAATTATGTAGCTCGTTCGGAAGGCGCATCTGCTCTTGCTTCGGTCAATCCGTTGAAGACTACCGTTG
AAGAGGCGCTTTCTCGTGGTTGGAGCGTGAAGAGCGGTACAGGTACAGAGGACGCTACTAAGAAAGAGGTTCCTCTGGGG
GTGGCGGCAGATGCTAACAAACTGGGTACTATCGCACTCAAACCCGATCCTGCTGATGGTACTGCAGATATTACTTTGAC
TTTCACTATGGGCGGTGCAGGACCGAAGAATAAAGGGAAAATTATTACCCTGACTCGTACTGCAGCTGATGGTCTCTGGA
AGTGCACCAGTGATCAGGATGAGCAGTTTATTCCGAAAGGTTGCTCTAAGTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A4GRK7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

100

100

1
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.682

100

0.48
  pilA Acinetobacter baumannii strain A118

45.333

100

0.453
  pilA/pilAII Pseudomonas stutzeri DSM 10701

46.575

97.333

0.453
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.675

100

0.447
  pilA Vibrio cholerae C6706

42.675

100

0.447
  pilA Vibrio cholerae strain A1552

42.675

100

0.447
  pilA Vibrio parahaemolyticus RIMD 2210633

48.507

89.333

0.433
  comP Acinetobacter baylyi ADP1

40.789

100

0.413
  pilA2 Legionella pneumophila strain ERS1305867

39.865

98.667

0.393
  pilA/pilA1 Eikenella corrodens VA1

39.456

98

0.387
  pilA2 Legionella pneumophila str. Paris

39.189

98.667

0.387