Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   J4G50_RS02155 Genome accession   NZ_CP071825
Coordinates   450751..452256 (-) Length   501 a.a.
NCBI ID   WP_096507359.1    Uniprot ID   A0A2A4C7Q8
Organism   Burkholderia anthina strain 1CH1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 445751..457256
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4G50_RS02130 (J4G50_02130) - 446780..447415 (-) 636 WP_096507361.1 LON peptidase substrate-binding domain-containing protein -
  J4G50_RS02135 (J4G50_02135) rapZ 447442..448350 (-) 909 WP_096507360.1 RNase adapter RapZ -
  J4G50_RS02140 (J4G50_02140) hprK 448426..449394 (-) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  J4G50_RS02145 (J4G50_02145) - 449524..450036 (-) 513 WP_059451610.1 PTS sugar transporter subunit IIA -
  J4G50_RS02150 (J4G50_02150) raiA 450232..450591 (-) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  J4G50_RS02155 (J4G50_02155) rpoN/rpoN1 450751..452256 (-) 1506 WP_096507359.1 RNA polymerase factor sigma-54 Machinery gene
  J4G50_RS02160 (J4G50_02160) lptB 452426..453202 (-) 777 WP_096507358.1 LPS export ABC transporter ATP-binding protein -
  J4G50_RS02165 (J4G50_02165) lptA 453199..453864 (-) 666 WP_096507357.1 lipopolysaccharide transport periplasmic protein LptA -
  J4G50_RS02170 (J4G50_02170) lptC 453885..454499 (-) 615 WP_207997894.1 LPS export ABC transporter periplasmic protein LptC -
  J4G50_RS02175 (J4G50_02175) - 454503..455039 (-) 537 WP_207997895.1 HAD family hydrolase -
  J4G50_RS02180 (J4G50_02180) kdsD 455039..456022 (-) 984 WP_096507354.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54775.90 Da        Isoelectric Point: 4.8767

>NTDB_id=548116 J4G50_RS02155 WP_096507359.1 450751..452256(-) (rpoN/rpoN1) [Burkholderia anthina strain 1CH1]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQSTEPAA
ANGSSQSGERAERDEPAGADEYDGYASGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTASLDEVLADLPAELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHTLIRSLEPFPGAAYGKAEADYVVPDIIVKKSGQSWLAELNPEVMP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=548116 J4G50_RS02155 WP_096507359.1 450751..452256(-) (rpoN/rpoN1) [Burkholderia anthina strain 1CH1]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATTTGGCGCTGACGCCGCAGCTACAGCAGTCGATCCGGCTTCTGCA
GTTGTCGACGCTCGAACTGCAACAGGAAGTCGCGATGGCCGTCGCGCAGAATCCGCTGCTCGAAAACGACGATGAATGGA
TCGCGAGTCCGCTGCGTGTCGCGGCGGATGGATCGCTGATCGCGCAGACGCCGCCCGCGCAAAGCACCGAACCGGCCGCC
GCGAACGGCAGCAGCCAGTCGGGCGAGCGCGCCGAGCGCGACGAACCGGCCGGCGCCGACGAATACGACGGCTACGCATC
CGGCGACGGCAACGACGGCCCGCAGTGGAATCTCGACGAGTTCGGCCGCTCGAGCGGCGCATCGGACGACGACGACCTGC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCATCTCTCCGCGCAACTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCGTCGCTCGACGAAGTGCT
CGCCGATCTGCCGGCCGAACTCGAAATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCCG
CCGGGGTCGGTGCGCGCTCGGCATCCGAATGCCTGAAGCTGCAGCTGCTGCGCCTCGATCCGTCCCCCACTCGCACGCTC
GCGCTCGAAATCGTGTCGCAGCATCTCGAACTGCTCGCCGCGCGCGATTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACACGCTGATCCGCTCGCTGGAGCCGTTCCCGGGCGCCGCCTACGGCAAGGCCGAAG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGTCCGGCCAGAGCTGGCTCGCGGAACTCAATCCGGAAGTGATGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGTTGAAACAGCA
GCTCCAGGAAGCGCGCTGGCTGATCAAGAACATCCAGCAGCGCTTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AACGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACAGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCACACGTTTCGACCGACACCGGAGGCGCCGCTTCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTTGCGCGC
CGCACGGTCGCCAAGTATCGCGAAGCCCTCAAAATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A4C7Q8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.775

100

0.695