Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   EXW53_RS24960 Genome accession   NZ_CP071808
Coordinates   4828352..4829701 (-) Length   449 a.a.
NCBI ID   WP_215557703.1    Uniprot ID   -
Organism   Bacillus mycoides strain JAS014     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4823352..4834701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW53_RS24935 (EXW53_25000) - 4824737..4825675 (-) 939 WP_002112865.1 YafY family protein -
  EXW53_RS24940 (EXW53_25005) - 4825758..4826201 (+) 444 WP_002198855.1 VOC family protein -
  EXW53_RS24945 (EXW53_25010) raiA 4826460..4827002 (-) 543 WP_002159874.1 ribosome-associated translation inhibitor RaiA -
  EXW53_RS24950 (EXW53_25015) cspC 4827324..4827521 (-) 198 WP_033710669.1 cold shock protein CspC -
  EXW53_RS24955 (EXW53_25020) - 4827648..4828352 (-) 705 WP_215557702.1 ComF family protein -
  EXW53_RS24960 (EXW53_25025) comFA 4828352..4829701 (-) 1350 WP_215557703.1 ATP-dependent helicase ComFA Machinery gene
  EXW53_RS24965 (EXW53_25030) - 4829832..4831169 (-) 1338 WP_215557704.1 C40 family peptidase -
  EXW53_RS24970 (EXW53_25035) - 4831402..4831716 (-) 315 WP_002112882.1 helix-turn-helix domain-containing protein -
  EXW53_RS24975 (EXW53_25040) - 4831890..4832732 (-) 843 WP_002112883.1 DegV family protein -
  EXW53_RS24980 (EXW53_25045) - 4832971..4833606 (+) 636 WP_002198851.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 50885.37 Da        Isoelectric Point: 9.6093

>NTDB_id=548032 EXW53_RS24960 WP_215557703.1 4828352..4829701(-) (comFA) [Bacillus mycoides strain JAS014]
MLAGRQLLLEELSSDLQGKLEHLKKNRDVVCVQGVIKKSSKYMCQRCGNIEQRLFVSFLCKRCNKVCTYCRKCITMGRVS
ECAVLVRGIAEISGENYLNPLQWEGVLSAGQELAAQSVVDAVKQKESFFIWAVCGAGKTEMLFRGIAEALQKGERVCIAT
PRTDVVLELAPRLQEVFPNINVAALYGGSLDREKDAALIVATTHQLLRYYRAFHVMIVDEIDAFPYHADKMLHYAVNEAM
KEEAARIYLTATPDEKWKRKLRNGKQKGVIISGRYHRHPLPVPIFRWCGNWKKGLMRKKIPRALLQWLNMYLSKRYPIFL
FVPHVRYVEEISQLLKSLNNQIDGVHAEDPARKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSSDEPHGDVIYFHYGKTEAMVRAKKHIQSMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=548032 EXW53_RS24960 WP_215557703.1 4828352..4829701(-) (comFA) [Bacillus mycoides strain JAS014]
ATGTTAGCTGGTAGACAGTTATTGTTAGAAGAACTTTCTTCAGATTTGCAGGGTAAATTGGAGCATTTGAAAAAGAATCG
AGACGTCGTTTGTGTACAAGGGGTAATTAAGAAATCTTCAAAATATATGTGCCAACGCTGCGGAAATATAGAGCAGCGAC
TATTTGTATCATTTCTATGTAAAAGGTGCAATAAAGTATGTACGTATTGCCGGAAGTGCATAACGATGGGCAGGGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAATAAGCGGAGAAAATTATTTGAATCCGTTACAGTGGGAAGGTGTTTT
GTCTGCTGGTCAGGAGTTAGCTGCGCAAAGTGTCGTTGACGCTGTTAAGCAGAAAGAATCATTTTTTATTTGGGCGGTGT
GCGGGGCTGGAAAAACAGAAATGTTGTTTCGCGGAATTGCAGAAGCACTACAAAAGGGAGAGAGAGTTTGTATTGCAACG
CCGAGAACGGACGTTGTACTTGAATTAGCACCAAGGTTACAAGAAGTGTTTCCAAATATAAATGTAGCTGCTTTATACGG
AGGGAGTTTAGATCGAGAAAAAGATGCGGCGTTAATCGTTGCAACTACACATCAATTGTTACGTTATTATAGGGCGTTCC
ATGTCATGATTGTAGATGAGATAGATGCTTTTCCGTATCATGCGGATAAGATGTTGCATTATGCAGTAAACGAAGCGATG
AAAGAGGAGGCAGCACGTATTTATTTAACTGCAACTCCAGATGAAAAGTGGAAGCGTAAATTGCGGAATGGTAAGCAAAA
AGGGGTTATTATTTCAGGACGTTATCATCGTCATCCATTGCCAGTCCCGATATTTCGGTGGTGCGGAAATTGGAAAAAGG
GTCTTATGCGTAAAAAAATTCCTCGTGCTTTATTACAATGGTTAAATATGTACTTATCTAAACGATACCCCATTTTTCTA
TTTGTTCCTCATGTGCGATATGTAGAAGAAATTAGTCAGTTATTAAAATCATTAAACAATCAAATTGACGGTGTGCATGC
AGAAGATCCGGCGAGAAAAGAGAAGGTAGCAGCTTTCAGAAAGGGAGAAATCCCTTTATTAGTTACAACGACAATTTTGG
AACGAGGAGTAACTGTGAAAAATTTACAAGTGGCAGTTTTAGGAGCTGAGGAAGAAATATTTTCAGAAAGTGCTCTCGTT
CAAATTGCAGGGCGTGCTGGCAGAAGCTCTGATGAACCGCATGGAGATGTCATCTATTTTCATTATGGAAAGACAGAGGC
GATGGTGCGTGCGAAAAAACATATTCAAAGTATGAATAAGAATGCTAAAGAACAAGGATTGATAGATTGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(107-254)

Helicase conserved C-terminal domain (Helicase_C)

(313-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

50.794

98.218

0.499
  comFA Latilactobacillus sakei subsp. sakei 23K

41.206

88.641

0.365