Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   EXW53_RS20740 Genome accession   NZ_CP071808
Coordinates   4029924..4030670 (-) Length   248 a.a.
NCBI ID   WP_002199331.1    Uniprot ID   J7XT79
Organism   Bacillus mycoides strain JAS014     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 4024924..4035670
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW53_RS20720 (EXW53_20775) dnaG 4025654..4027456 (-) 1803 WP_002067179.1 DNA primase -
  EXW53_RS27565 - 4027657..4027950 (-) 294 WP_252188333.1 DUF188 domain-containing protein -
  EXW53_RS20730 (EXW53_20785) - 4028343..4029155 (-) 813 WP_002111882.1 pyruvate, water dikinase regulatory protein -
  EXW53_RS20735 (EXW53_20790) - 4029185..4029817 (-) 633 WP_087945800.1 helix-turn-helix transcriptional regulator -
  EXW53_RS20740 (EXW53_20795) recO 4029924..4030670 (-) 747 WP_002199331.1 DNA repair protein RecO Machinery gene
  EXW53_RS20745 (EXW53_20800) - 4030704..4030847 (-) 144 WP_000883924.1 YqzL family protein -
  EXW53_RS20750 (EXW53_20805) era 4031024..4031929 (-) 906 WP_002122584.1 GTPase Era -
  EXW53_RS20755 (EXW53_20810) - 4031922..4032320 (-) 399 WP_215557477.1 cytidine deaminase -
  EXW53_RS20760 (EXW53_20815) - 4032420..4032773 (-) 354 WP_215557478.1 diacylglycerol kinase family protein -
  EXW53_RS20765 (EXW53_20820) ybeY 4032770..4033240 (-) 471 WP_215557479.1 rRNA maturation RNase YbeY -
  EXW53_RS20770 (EXW53_20825) - 4033237..4035381 (-) 2145 WP_002199327.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28616.18 Da        Isoelectric Point: 8.3250

>NTDB_id=548022 EXW53_RS20740 WP_002199331.1 4029924..4030670(-) (recO) [Bacillus mycoides strain JAS014]
MFQKVEGIVIRTTDYGETNKIVTIYSREFGKVSAMARGAKKPKSRLAAISQLMTHGHFLIQIGSGLGTLQQGEIISSMKE
IRKDIFLTAYASFIIELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMHPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDPYRIPVGEAVHKLLRLFFHFDLHRLGNVSVKDSTKKQMRTVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=548022 EXW53_RS20740 WP_002199331.1 4029924..4030670(-) (recO) [Bacillus mycoides strain JAS014]
ATGTTTCAAAAAGTTGAGGGCATTGTCATCCGTACAACAGATTACGGAGAAACAAATAAGATTGTTACAATATACTCACG
GGAATTTGGTAAGGTAAGTGCAATGGCAAGAGGGGCGAAAAAACCGAAGAGTCGGTTAGCGGCTATTTCGCAACTTATGA
CACATGGTCATTTTCTTATTCAAATTGGATCTGGACTTGGAACTTTGCAACAAGGCGAGATTATTTCATCTATGAAAGAA
ATTCGCAAGGATATATTTTTAACTGCTTATGCATCATTTATTATCGAATTAACTGATAAAGCAACAGAAGATAAGAAGCA
TAATCCGTATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCACCCGTACTTTGATACATGTGCTATTTGTCATCAAGAAACAGAT
TTTGTCGCCTTCTCTGTCAGGGAAGGCGGTTTTCTGTGCTCGCGTCATGCGGAGCAAGATCCGTATCGTATTCCGGTGGG
AGAGGCTGTTCATAAATTATTACGTCTCTTTTTTCACTTCGATCTACACCGGCTCGGTAATGTATCGGTGAAGGATAGTA
CAAAAAAACAAATGCGCACAGTATTGAATACATATTATGATGAATATTGTGGGATTTATTTGAAATCAAGGCGTTTCCTG
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J7XT79

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552