Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EXW53_RS19255 Genome accession   NZ_CP071808
Coordinates   3771221..3771655 (-) Length   144 a.a.
NCBI ID   WP_002014817.1    Uniprot ID   -
Organism   Bacillus mycoides strain JAS014     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3766221..3776655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW53_RS19240 (EXW53_19295) - 3767385..3767942 (+) 558 WP_002160661.1 PadR family transcriptional regulator -
  EXW53_RS19245 (EXW53_19300) metE 3768463..3770751 (+) 2289 WP_215557410.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  EXW53_RS19250 (EXW53_19305) comJ 3770792..3771181 (-) 390 WP_215557411.1 competence protein ComJ -
  EXW53_RS19255 (EXW53_19310) nucA/comI 3771221..3771655 (-) 435 WP_002014817.1 DNA-entry nuclease Machinery gene
  EXW53_RS19260 (EXW53_19315) - 3771892..3772635 (+) 744 WP_002111539.1 DUF3967 domain-containing protein -
  EXW53_RS19265 (EXW53_19320) - 3772695..3773969 (-) 1275 WP_002111540.1 HAMP domain-containing sensor histidine kinase -
  EXW53_RS19270 (EXW53_19325) - 3774279..3774479 (+) 201 WP_002111542.1 hypothetical protein -
  EXW53_RS19275 (EXW53_19330) - 3774700..3775863 (+) 1164 WP_002111544.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16194.59 Da        Isoelectric Point: 8.4331

>NTDB_id=548018 EXW53_RS19255 WP_002014817.1 3771221..3771655(-) (nucA/comI) [Bacillus mycoides strain JAS014]
MKQFKGIIISIIAILSLLVAVYEVLVPEETSTKKTTTYDQILEFPKERYPETGKHITDAIKEGHSEMCTIDRNGAADRRK
LSLAPYPTKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=548018 EXW53_RS19255 WP_002014817.1 3771221..3771655(-) (nucA/comI) [Bacillus mycoides strain JAS014]
ATGAAGCAATTTAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTCTTTTGGTAGCGGTATATGAAGTACTTGTGCC
AGAGGAGACAAGTACTAAAAAAACAACTACGTATGATCAAATTCTAGAGTTCCCGAAAGAACGATATCCAGAGACCGGGA
AACATATAACGGATGCTATAAAAGAAGGGCATTCAGAAATGTGCACAATTGATCGTAATGGTGCAGCGGATAGAAGGAAA
TTATCGTTAGCGCCATATCCGACGAAGAAAGGTTATGATCGTGATGAATGGCCGATGGCGATGTGTAAGGAGGGTGGAAA
AGGAGCCCATATTGAATATATAAGTCCAGCAGATAACCGCGGTGCAGGTTCTTGGGTAGGGAATAAGCTAGACAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAATAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(47-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.027

78.472

0.542