Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EXW53_RS00530 Genome accession   NZ_CP071808
Coordinates   97581..98957 (+) Length   458 a.a.
NCBI ID   WP_002113294.1    Uniprot ID   -
Organism   Bacillus mycoides strain JAS014     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 92581..103957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW53_RS00510 (EXW53_00510) ctsR 92780..93241 (+) 462 WP_002202060.1 transcriptional regulator CtsR -
  EXW53_RS00515 (EXW53_00515) - 93411..93959 (+) 549 WP_002113290.1 UvrB/UvrC motif-containing protein -
  EXW53_RS00520 (EXW53_00520) - 93964..95028 (+) 1065 WP_002202059.1 protein arginine kinase -
  EXW53_RS00525 (EXW53_00525) clpC 95051..97486 (+) 2436 WP_215557795.1 ATP-dependent Clp protease ATP-binding subunit Regulator
  EXW53_RS00530 (EXW53_00530) radA 97581..98957 (+) 1377 WP_002113294.1 DNA repair protein RadA Machinery gene
  EXW53_RS00535 (EXW53_00535) disA 98961..100034 (+) 1074 WP_002202058.1 DNA integrity scanning diadenylate cyclase DisA -
  EXW53_RS00540 (EXW53_00540) - 100196..101299 (+) 1104 WP_002113296.1 PIN/TRAM domain-containing protein -
  EXW53_RS00545 (EXW53_00545) ispD 101316..101996 (+) 681 WP_002202057.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  EXW53_RS00550 (EXW53_00550) ispF 102110..102586 (+) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49828.56 Da        Isoelectric Point: 7.7443

>NTDB_id=547994 EXW53_RS00530 WP_002113294.1 97581..98957(+) (radA) [Bacillus mycoides strain JAS014]
MAKKKTKFTCQECGYQSPKYMGKCPGCGKWNTLVEEMEPVVSSRRLNYANAIQSEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVKGSNLFVVAETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKATAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=547994 EXW53_RS00530 WP_002113294.1 97581..98957(+) (radA) [Bacillus mycoides strain JAS014]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGTTATCAGTCGCCAAAATATATGGGGAAATGTCCTGGATG
TGGTAAATGGAATACGCTTGTTGAAGAGATGGAACCAGTTGTATCATCAAGGCGCCTTAATTATGCCAATGCAATTCAAT
CAGAAGTAACAAAACCAAGACGCTTAACAGAGGTAGAAACAAAGTCTGAGGCGCGTATTGAAACGAAATTTCAAGAGTTT
AACCGTGTACTTGGTGGCGGGATTGTAGATGGATCTTTAGTACTTATTGGTGGAGACCCTGGGATTGGGAAATCAACATT
GTTATTACAAATTTCATCGCAATTAGCAGATTCTTCATATGATGTACTATACATATCAGGTGAGGAGTCAGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTGCATGTAAAGGGTAGTAATCTATTTGTTGTAGCAGAAACTGATCTACAGCGAATTGCA
GCGCACATTGAAGAGATGAATCCAGCTTTTGTTGTTATTGACTCTATTCAAACGATACATTTACCTGAAGTGACGTCAGC
CCCAGGAAGTGTAGCGCAAGTACGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACGAAAGGGATTCCTATTTTTA
TCGTCGGACATGTGACAAAAGAAGGGGCAATTGCAGGACCACGTATGCTAGAACATATGGTTGATGCAGTTCTTTACTTT
GAAGGAGATCGTCATCATACATATCGTATTTTGCGAGCTGTGAAGAACCGTTTTGGTTCCACAAATGAAATGGGTATCTT
TGAAATGAAGGAACTAGGTCTTGCAGAAGTACTAAATCCTTCTGAAATTTTCCTTGAGGAACGTCCAGTTGGAGTTGCAG
GATCCACAGTGGTTGCTTCAATGGAAGGAACAAGACCGGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCGACGGGAATTGATCATAACCGCGTTTCACTTATTATGGCAGTGCTAGAAAAAAGAACAGG
TTTATTGTTGCAAAATCAAGACGCGTATTTAAAAGTCGCGGGTGGCTTGAAATTAGATGAACCAGCAATCGATTTAGCTG
TTGCCTTAAGTATTGCTTCAAGTTTCAGAGATAAAGCTACTGCACCAACCGATGCGGTAATAGGAGAAGTAGGCCTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCAAAATTAGGATTTCAACGTGCTATTATTCC
TAGAAAAAATTTGGGAGGATGGACAATTCCAGATGGGATTGAGGTAGTAGGTGTTTCTAATTTAGGAGAGGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.51

100

0.747
  radA Streptococcus pneumoniae Rx1

64.018

98.908

0.633
  radA Streptococcus pneumoniae D39

64.018

98.908

0.633
  radA Streptococcus pneumoniae R6

64.018

98.908

0.633
  radA Streptococcus pneumoniae TIGR4

64.018

98.908

0.633
  radA Streptococcus mitis SK321

64.018

98.908

0.633
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631