Detailed information NTG0547842

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: LSI70_RS01430
• Genome accession: NZ_CP089034
• Coordinates: 286317..287300 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli O157:H7 strain 6535NalR
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 281317..292300

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LSI70_RS01415 (LSI70_01415)	dppA	282617..284224 (+)	1608	WP_001222888.1	dipeptide ABC transporter substrate-binding protein DppA	-
LSI70_RS01420 (LSI70_01420)	dppB	284375..285394 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
LSI70_RS01425 (LSI70_01425)	dppC	285404..286306 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
LSI70_RS01430 (LSI70_01430)	amiE	286317..287300 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
LSI70_RS01435 (LSI70_01435)	dppF	287297..288301 (+)	1005	WP_000107027.1	dipeptide ABC transporter ATP-binding subunit DppF	-
LSI70_RS01440 (LSI70_01440)	yhjV	288331..289602 (-)	1272	WP_001301684.1	aromatic amino acid transport family protein	-
LSI70_RS01445 (LSI70_01445)	ldrD	290078..290185 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
LSI70_RS01450 (LSI70_01450)	bcsG	290272..291951 (-)	1680	WP_000191596.1	cellulose biosynthesis protein BcsG	-
LSI70_RS01455 (LSI70_01455)	bcsF	291948..292139 (-)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=547842 LSI70_RS01430 WP_001196486.1 286317..287300(+) (amiE) [Escherichia coli O157:H7 strain 6535NalR]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=547842 LSI70_RS01430 WP_001196486.1 286317..287300(+) (amiE) [Escherichia coli O157:H7 strain 6535NalR]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGACCGCATTAG
CTACAGCGTAAAACAGGGCGAAGTAGTCGGGATTGTGGGTGAGTCTGGCTCCGGTAAGTCGGTCAGTTCGCTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTGATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCGGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGTAATAAGAGCACTCGTCGTCAGCGGGCGATTGACT
TGTTGAATCAGGTCGGTATTCCCGATCCGGCTTCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATTGCGATGGCCATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATTGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCCGGTCAGGTGGTGGAAACGGGCGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCATCGTTGCCTGGCGTTGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCTTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404