Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   J1M35_RS02700 Genome accession   NZ_CP071796
Coordinates   596444..598024 (+) Length   526 a.a.
NCBI ID   WP_208009629.1    Uniprot ID   -
Organism   Ottowia testudinis strain 27C     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 591444..603024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J1M35_RS02680 (J1M35_02680) - 593051..593857 (+) 807 WP_208009626.1 SPOR domain-containing protein -
  J1M35_RS02685 (J1M35_02685) - 594077..594712 (+) 636 WP_208009627.1 thiol:disulfide interchange protein DsbA/DsbL -
  J1M35_RS02690 (J1M35_02690) lptA 594860..595501 (+) 642 WP_208009628.1 lipopolysaccharide transport periplasmic protein LptA -
  J1M35_RS02695 (J1M35_02695) lptB 595555..596316 (+) 762 WP_208011132.1 LPS export ABC transporter ATP-binding protein -
  J1M35_RS02700 (J1M35_02700) rpoN/rpoN1 596444..598024 (+) 1581 WP_208009629.1 RNA polymerase factor sigma-54 Machinery gene
  J1M35_RS02705 (J1M35_02705) - 598102..598734 (+) 633 WP_208009630.1 biliverdin-producing heme oxygenase -
  J1M35_RS02710 (J1M35_02710) - 598731..600878 (+) 2148 WP_208009632.1 GAF domain-containing protein -
  J1M35_RS02715 (J1M35_02715) - 600924..602246 (+) 1323 WP_208009633.1 THUMP domain-containing protein -
  J1M35_RS02720 (J1M35_02720) - 602271..602720 (+) 450 WP_243457561.1 putative toxin-antitoxin system toxin component, PIN family -

Sequence

Protein


Download         Length: 526 a.a.        Molecular weight: 57223.10 Da        Isoelectric Point: 4.7083

>NTDB_id=547802 J1M35_RS02700 WP_208009629.1 596444..598024(+) (rpoN/rpoN1) [Ottowia testudinis strain 27C]
MKPGLSLRVSQHLALTPQLQQSIRLLQLSTLELASEVEQMLDDNPFLERGDDSAPREEFGLTRADARVSDGDRIQETATE
NEAAGDDSTSAESGFDAESSVPDSWEGDGSVELRPDDGEWGGDAPARANNLGDDDELDPGERGGGQESLAEHLSRQALSL
RLSPEDTAALRFLIESLNDDGYLEDSLAQLAATLTDTDDDLEHLEELVHRFTMALKLLQSLEPAGVGARHLAECLALQLK
SRQAERDVGADERADIAVALKICAQHGAVDLLARRDAKRLAQLTCESEAAVRGAMARIGRLDPKPGRQFADVERNAIVPD
VIVSSAGGGGQARFRVQLNPDVMPKLRVHDVYANALRGGKGGDNHQALAQRLQEARWFIKNIQQRFDTILRVSQAIVARQ
SNFFIHGEVAMRPMVQRELADELGVHESTISRVTTAKYMATPRGTFELKYFFGSALGTEAGGNASSTAVRALIKQLIAAE
DPAKPLSDSQLAEMLKEQGIDCARRTVAKYREGLRIPVATLRKIVA

Nucleotide


Download         Length: 1581 bp        

>NTDB_id=547802 J1M35_RS02700 WP_208009629.1 596444..598024(+) (rpoN/rpoN1) [Ottowia testudinis strain 27C]
GTGAAACCGGGGCTGTCTCTCCGCGTCTCGCAGCATTTGGCGCTCACGCCGCAACTGCAGCAGTCGATTCGGCTGCTGCA
ACTGTCCACGCTCGAGCTGGCAAGCGAGGTCGAGCAGATGCTCGACGACAACCCCTTTCTGGAGCGCGGCGACGACAGCG
CGCCGCGCGAGGAATTCGGCCTGACACGTGCCGACGCGCGCGTCAGCGACGGCGATCGAATTCAAGAGACTGCTACAGAA
AACGAAGCTGCTGGCGATGATTCCACAAGCGCTGAATCCGGTTTTGATGCTGAATCCTCCGTGCCCGACAGCTGGGAGGG
CGATGGCAGCGTCGAACTGCGGCCCGACGACGGTGAATGGGGCGGCGATGCCCCGGCCCGCGCCAACAATCTGGGCGACG
ACGACGAACTCGACCCCGGCGAGCGTGGCGGCGGCCAGGAGTCGCTGGCCGAGCACCTGAGCCGCCAGGCCTTGTCGCTG
CGCCTGTCGCCCGAGGACACGGCGGCACTGCGCTTTCTGATCGAATCGCTGAACGACGATGGCTACCTTGAGGACTCGCT
GGCCCAGCTTGCCGCCACGCTGACCGATACCGACGACGACCTGGAGCATCTGGAGGAGCTGGTGCATCGATTCACGATGG
CCTTGAAGCTGCTGCAATCGCTGGAGCCGGCGGGCGTGGGCGCGCGCCACCTCGCCGAGTGCCTGGCGCTGCAGCTGAAA
AGCCGCCAGGCCGAGCGCGATGTGGGGGCCGATGAGCGGGCCGACATCGCCGTCGCGCTGAAAATTTGCGCGCAGCACGG
GGCTGTCGACCTGCTGGCCCGGCGCGACGCCAAGCGCCTGGCGCAGCTCACATGCGAAAGCGAAGCCGCGGTGCGCGGCG
CCATGGCGCGCATTGGCCGGCTCGACCCCAAGCCGGGCCGCCAGTTTGCCGACGTCGAGCGCAATGCCATCGTGCCCGAC
GTCATCGTCTCCAGCGCCGGCGGTGGCGGTCAAGCGCGCTTTCGCGTGCAGCTCAACCCCGACGTCATGCCCAAGTTGCG
CGTGCACGACGTCTATGCCAACGCACTGCGGGGCGGCAAGGGCGGAGACAACCACCAGGCGCTCGCCCAGCGCTTGCAGG
AGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCTTGCGCGTGTCGCAGGCCATCGTCGCGCGGCAG
AGCAATTTTTTCATCCATGGCGAGGTGGCGATGCGGCCCATGGTGCAGCGCGAGCTGGCCGACGAACTGGGCGTGCACGA
ATCCACCATCAGCCGCGTGACGACCGCCAAGTACATGGCCACGCCGCGCGGCACTTTCGAGCTGAAATATTTCTTCGGTT
CGGCGCTTGGCACCGAAGCCGGCGGCAACGCCAGCAGCACGGCGGTGCGCGCGCTCATCAAGCAGCTGATCGCCGCCGAA
GACCCGGCCAAGCCGCTGTCCGACAGCCAACTCGCCGAGATGCTGAAAGAGCAGGGCATCGACTGCGCGCGCCGCACGGT
GGCCAAATACCGCGAAGGCCTGCGCATCCCGGTGGCGACCCTGCGCAAAATCGTCGCCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(146-352)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(367-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

50.19

100

0.502