Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LSI69_RS03175 Genome accession   NZ_CP089032
Coordinates   643776..645209 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli O157:H7 strain 6535WT     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 638776..650209
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSI69_RS03140 (LSI69_03140) elbB 639467..640120 (+) 654 WP_001302019.1 isoprenoid biosynthesis glyoxalase ElbB -
  LSI69_RS03145 (LSI69_03145) mtgA 640117..640845 (+) 729 WP_000047079.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  LSI69_RS03150 (LSI69_03150) yrbL 640842..641474 (-) 633 WP_000620409.1 PhoP regulatory network protein YrbL -
  LSI69_RS03155 (LSI69_03155) npr 641688..641960 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  LSI69_RS03160 (LSI69_03160) rapZ 641957..642811 (-) 855 WP_000243741.1 RNase adapter RapZ -
  LSI69_RS03165 (LSI69_03165) ptsN 642857..643348 (-) 492 WP_001301839.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LSI69_RS03170 (LSI69_03170) hpf 643466..643753 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  LSI69_RS03175 (LSI69_03175) rpoN/rpoN1 643776..645209 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  LSI69_RS03180 (LSI69_03180) lptB 645257..645982 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  LSI69_RS03185 (LSI69_03185) lptA 645989..646546 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  LSI69_RS03190 (LSI69_03190) lptC 646515..647090 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  LSI69_RS03195 (LSI69_03195) kdsC 647087..647653 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  LSI69_RS03200 (LSI69_03200) kdsD 647674..648660 (-) 987 WP_001302021.1 arabinose-5-phosphate isomerase KdsD -
  LSI69_RS03205 (LSI69_03205) yrbG 648674..649651 (-) 978 WP_000922872.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=547772 LSI69_RS03175 WP_000809051.1 643776..645209(-) (rpoN/rpoN1) [Escherichia coli O157:H7 strain 6535WT]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=547772 LSI69_RS03175 WP_000809051.1 643776..645209(-) (rpoN/rpoN1) [Escherichia coli O157:H7 strain 6535WT]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAATCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCTCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAATGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAAGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465