Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   OSCIL6304_RS03395 Genome accession   NC_019693
Coordinates   853261..855276 (-) Length   671 a.a.
NCBI ID   WP_015147077.1    Uniprot ID   -
Organism   Oscillatoria acuminata PCC 6304     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 848261..860276
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OSCIL6304_RS03370 (Oscil6304_0683) - 848607..849068 (+) 462 WP_015147072.1 ferredoxin -
  OSCIL6304_RS03375 (Oscil6304_0684) - 849197..849475 (-) 279 WP_015147073.1 YiaA/YiaB family inner membrane protein -
  OSCIL6304_RS36245 - 849733..849864 (-) 132 WP_284690273.1 hypothetical protein -
  OSCIL6304_RS03380 (Oscil6304_0685) - 849977..850588 (-) 612 WP_015147074.1 hypothetical protein -
  OSCIL6304_RS03385 (Oscil6304_0686) - 850768..851895 (-) 1128 WP_015147075.1 type II secretion system F family protein -
  OSCIL6304_RS03390 (Oscil6304_0687) - 852085..853185 (-) 1101 WP_015147076.1 type IV pilus twitching motility protein PilT -
  OSCIL6304_RS03395 (Oscil6304_0688) pilB/pilB1 853261..855276 (-) 2016 WP_015147077.1 GspE/PulE family protein Machinery gene
  OSCIL6304_RS03400 (Oscil6304_0689) - 856087..856290 (-) 204 WP_015147078.1 hypothetical protein -
  OSCIL6304_RS03405 (Oscil6304_0690) - 856572..856793 (-) 222 WP_015147079.1 hypothetical protein -
  OSCIL6304_RS03410 (Oscil6304_0691) - 856960..857286 (-) 327 WP_015147080.1 NfeD family protein -
  OSCIL6304_RS03415 (Oscil6304_0692) - 857660..859525 (+) 1866 WP_015147081.1 DNA-directed RNA polymerase specialized sigma subunit -

Sequence

Protein


Download         Length: 671 a.a.        Molecular weight: 74185.58 Da        Isoelectric Point: 5.0547

>NTDB_id=54772 OSCIL6304_RS03395 WP_015147077.1 853261..855276(-) (pilB/pilB1) [Oscillatoria acuminata PCC 6304]
MTNFASQRRALVVQNAFSPFGNKLIQAGYADREQVQRAHIESRKSGKPLTEVLEAITGKQLPPELLRQYKKQQLFELKIV
YGVEALDPEITELSPEQLGPLLETLLPLESCRRHRILPISRNEGESPSVSVAMVDPDNLAALDDLTRILRPQGLALRRLV
ITPEDYNNLLNQYSDEKAKEQQAKAEKANQLDLNLNSDVFNEVDGESLDDAPEEDEDMNLDAALRGANDAPIINTVNKIL
AKALSDGVSDIHVEPQEEFLRIRMRKDGVLQEFARLPSKITNALTSRFKIISDMDIAERRNAQDGRIRRIFQGRTVDFRV
NCLPSRYGEKLCLRILDNSSTQLGLDKLISNQESLALVREMASRPFGLILVTGPTGSGKSTSLYSVLAERNDPAVNISTA
EDPIEYALPGITQCQVIRAKGLDFTNLLRAFMRQDPDIILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIARLD
EMGVEPFMVAGSLIGVLAQRLMRRVCGECRIAYTPTPEELGRFGLTVSGETNITFYKANSIPPADRKAAQDAGTLCQKCG
GGGYKGRVGVYEVLKNTENLQMLISQGAPTERIKEAAVEEGMVTLLAYSLNLVREGHTTFEEVERVTFTDSGLEAELKAK
RKTGLTCKACGAGLQQEWLDCLYCLTPRFQE

Nucleotide


Download         Length: 2016 bp        

>NTDB_id=54772 OSCIL6304_RS03395 WP_015147077.1 853261..855276(-) (pilB/pilB1) [Oscillatoria acuminata PCC 6304]
ATGACAAACTTCGCATCACAGCGGCGTGCCCTAGTTGTCCAGAATGCATTTTCTCCCTTCGGCAATAAGCTCATCCAAGC
GGGCTATGCCGATCGCGAACAAGTTCAACGCGCCCACATTGAAAGTCGGAAATCAGGGAAGCCTCTTACCGAGGTCCTCG
AAGCCATTACTGGAAAGCAACTGCCTCCAGAATTGCTCAGGCAGTACAAGAAGCAGCAGCTTTTTGAACTGAAAATCGTC
TACGGCGTAGAAGCCTTGGATCCAGAAATCACCGAACTGTCACCGGAACAACTAGGTCCACTCCTAGAGACCTTGCTGCC
CCTGGAAAGTTGCCGTCGCCATCGCATCCTGCCCATCTCCCGCAACGAGGGCGAATCGCCTTCGGTTTCTGTAGCAATGG
TGGATCCGGATAACTTAGCCGCCCTGGACGACTTGACCCGGATCCTGCGACCCCAGGGACTAGCCCTCCGCCGACTGGTA
ATTACACCAGAAGACTATAACAACTTACTCAATCAATATTCTGACGAAAAGGCCAAAGAACAACAGGCCAAAGCAGAAAA
AGCTAATCAACTCGATTTAAACCTCAACTCTGACGTTTTCAATGAGGTAGATGGGGAATCCCTTGATGACGCCCCAGAAG
AAGATGAAGACATGAATCTGGATGCGGCGTTGCGCGGGGCCAACGACGCACCCATTATTAACACCGTTAATAAAATCTTG
GCCAAAGCCCTCTCCGATGGCGTCTCCGATATCCACGTCGAACCCCAAGAAGAGTTTCTGAGAATTCGGATGCGGAAAGA
CGGCGTACTTCAAGAGTTTGCCCGGTTACCCAGTAAAATTACAAATGCCTTGACTTCCCGGTTCAAAATTATTTCCGATA
TGGATATTGCCGAACGGCGGAATGCCCAGGATGGACGGATTCGGCGGATATTCCAGGGACGCACCGTGGACTTCCGGGTG
AACTGTCTGCCGTCGCGGTATGGTGAAAAACTCTGCTTACGGATTTTGGATAACTCCTCCACCCAGTTGGGATTGGATAA
ATTAATTTCCAATCAAGAGAGTTTGGCGCTAGTCCGAGAAATGGCGAGTCGTCCTTTTGGTCTGATTTTGGTGACGGGAC
CCACGGGTTCGGGGAAATCAACTTCCTTGTATTCAGTGTTAGCCGAACGGAACGACCCAGCGGTAAATATCTCGACCGCA
GAAGACCCGATTGAATATGCCTTGCCGGGGATTACCCAATGTCAAGTGATTCGGGCCAAAGGACTGGACTTTACTAACTT
ATTGCGGGCGTTCATGCGGCAAGACCCGGACATCATTCTGGTGGGTGAAACGCGGGATAAAGAAACGGCGAAAACGGCCA
TTGAAGCAGCCTTGACGGGTCACTTAGTGTTAACCACCCTGCATACCAACGACGCAGCCGGGGCGATCGCTCGTTTAGAT
GAAATGGGAGTTGAACCCTTCATGGTCGCCGGTTCTCTGATTGGTGTACTGGCCCAGCGCTTGATGCGGCGGGTCTGTGG
CGAATGTCGCATTGCCTACACCCCCACCCCGGAAGAACTTGGTCGCTTTGGACTCACTGTTTCTGGGGAAACCAATATCA
CCTTCTATAAAGCCAACTCCATTCCCCCAGCGGACCGTAAAGCCGCTCAAGACGCCGGGACTTTATGCCAGAAATGTGGG
GGCGGTGGATATAAAGGCCGGGTTGGGGTCTATGAAGTCCTCAAAAACACTGAAAATCTGCAAATGTTAATCTCTCAAGG
TGCGCCCACGGAACGGATTAAGGAAGCTGCCGTGGAAGAGGGGATGGTCACCTTGCTCGCCTATAGCCTCAACCTGGTTC
GGGAAGGGCATACCACTTTTGAAGAGGTTGAACGGGTTACTTTTACCGACTCTGGCTTGGAAGCAGAACTCAAAGCCAAA
CGCAAGACGGGTCTAACTTGTAAGGCTTGCGGGGCCGGATTGCAGCAGGAGTGGCTCGATTGCCTGTACTGCTTGACCCC
GCGCTTCCAAGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-177)

Type II/IV secretion system protein (T2SSE)

(238-504)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

67.068

99.106

0.665

Multiple sequence alignment