Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   J4G46_RS03405 Genome accession   NZ_CP071771
Coordinates   709522..710331 (+) Length   269 a.a.
NCBI ID   WP_004975096.1    Uniprot ID   A0AAP9GTD8
Organism   Acinetobacter towneri strain GX7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 704522..715331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4G46_RS03375 (J4G46_03375) - 704651..705076 (+) 426 WP_004975083.1 hypothetical protein -
  J4G46_RS03380 (J4G46_03380) - 705343..706626 (+) 1284 WP_179988668.1 glycosyltransferase family 1 protein -
  J4G46_RS03385 (J4G46_03385) - 706652..707203 (+) 552 WP_004975087.1 phosphatase PAP2 family protein -
  J4G46_RS03390 (J4G46_03390) iscX 707305..707505 (+) 201 WP_004975089.1 Fe-S cluster assembly protein IscX -
  J4G46_RS03395 (J4G46_03395) ndk 707667..708098 (+) 432 WP_004975092.1 nucleoside-diphosphate kinase -
  J4G46_RS03400 (J4G46_03400) rlmN 708235..709467 (+) 1233 WP_004975094.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  J4G46_RS03405 (J4G46_03405) pilF 709522..710331 (+) 810 WP_004975096.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  J4G46_RS03410 (J4G46_03410) - 710322..711131 (+) 810 WP_208012216.1 helix-turn-helix domain-containing protein -
  J4G46_RS03415 (J4G46_03415) ispG 711154..712275 (+) 1122 WP_004975100.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  J4G46_RS03420 (J4G46_03420) hisS 712279..713574 (+) 1296 WP_004975101.1 histidine--tRNA ligase -
  J4G46_RS03425 (J4G46_03425) - 713605..714306 (+) 702 WP_004975103.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 30278.37 Da        Isoelectric Point: 9.2819

>NTDB_id=547668 J4G46_RS03405 WP_004975096.1 709522..710331(+) (pilF) [Acinetobacter towneri strain GX7]
MNKPVFKIAQITTVCISAIFAAACQTTAPTLGQKDPEKAVQVRTQLAAEYLKNGDLDSAKRALDQALESDSRDSTANMMM
GVLLQQEGSKLNLEKADAYFKRAIAADPKNAQARNNYGTYLYQVERYNDAIEQLSIAGATLGYDQRFRALENVGRIYLKL
GDIENAEKSFKQALQANRDSYVSMLELSEILYLRQQFAPARQLYEQFVRAVGQKNQGARALWIGIRLARANGDQLGTQVL
VNQLRALFPNSQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 810 bp        

>NTDB_id=547668 J4G46_RS03405 WP_004975096.1 709522..710331(+) (pilF) [Acinetobacter towneri strain GX7]
TTGAATAAGCCTGTATTTAAAATCGCACAAATTACAACTGTTTGTATTTCGGCAATTTTTGCTGCAGCTTGTCAAACCAC
AGCTCCAACCTTAGGGCAAAAAGACCCTGAAAAAGCGGTGCAAGTACGTACGCAGTTGGCAGCAGAATACTTAAAAAATG
GCGATTTAGACTCAGCCAAACGCGCACTTGATCAAGCTTTGGAAAGCGATTCACGTGATTCCACCGCCAATATGATGATG
GGCGTGTTGCTGCAACAAGAAGGCAGCAAGCTGAATTTAGAAAAAGCCGATGCGTATTTTAAGCGTGCCATTGCCGCTGA
CCCAAAAAATGCACAAGCGCGTAACAACTATGGTACGTATTTATATCAAGTAGAACGCTATAATGATGCGATTGAGCAGC
TCAGTATTGCAGGTGCAACACTGGGTTATGATCAACGCTTTAGAGCATTGGAAAATGTAGGTCGTATTTACTTGAAATTA
GGCGATATAGAGAATGCCGAAAAGTCATTCAAACAAGCCTTGCAAGCCAATCGCGATTCTTATGTTTCAATGTTAGAGTT
GTCGGAAATTTTATATTTAAGACAACAATTTGCACCCGCGCGACAATTGTATGAACAATTTGTACGTGCTGTGGGGCAAA
AAAATCAGGGTGCGCGTGCCTTATGGATTGGGATTCGTTTAGCACGTGCCAATGGCGATCAATTGGGAACTCAGGTTTTA
GTGAACCAGCTGCGTGCATTATTTCCCAATAGCCAAGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGT
ATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(154-210)

Tetratricopeptide repeat (TPR_16)

(46-83)

Tetratricopeptide repeat (TPR_17)

(100-132)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

74.721

100

0.747
  pilF Acinetobacter baylyi ADP1

72.047

94.424

0.68