Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   J4G44_RS00055 Genome accession   NZ_CP071766
Coordinates   9398..10186 (-) Length   262 a.a.
NCBI ID   WP_371971077.1    Uniprot ID   -
Organism   Acinetobacter towneri strain GX3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4398..15186
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4G44_RS00035 (J4G44_00035) - 5423..6124 (-) 702 WP_096901519.1 YfgM family protein -
  J4G44_RS00040 (J4G44_00040) hisS 6155..7450 (-) 1296 WP_207973720.1 histidine--tRNA ligase -
  J4G44_RS00045 (J4G44_00045) ispG 7454..8575 (-) 1122 WP_207973721.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  J4G44_RS00050 (J4G44_00050) - 8598..9407 (-) 810 WP_207973722.1 helix-turn-helix domain-containing protein -
  J4G44_RS00055 (J4G44_00055) pilF 9398..10186 (-) 789 WP_371971077.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  J4G44_RS00060 (J4G44_00060) rlmN 10262..11494 (-) 1233 WP_004975094.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  J4G44_RS00065 (J4G44_00065) ndk 11631..12062 (-) 432 WP_004975092.1 nucleoside-diphosphate kinase -
  J4G44_RS00070 (J4G44_00070) iscX 12224..12424 (-) 201 WP_019836293.1 Fe-S cluster assembly protein IscX -
  J4G44_RS00075 (J4G44_00075) - 12526..13077 (-) 552 WP_004975087.1 phosphatase PAP2 family protein -
  J4G44_RS00080 (J4G44_00080) - 13103..14386 (-) 1284 WP_207973723.1 glycosyltransferase family 4 protein -
  J4G44_RS00085 (J4G44_00085) - 14653..15078 (-) 426 WP_004975083.1 hypothetical protein -

Sequence

Protein


Download         Length: 262 a.a.        Molecular weight: 29451.33 Da        Isoelectric Point: 8.7703

>NTDB_id=547569 J4G44_RS00055 WP_371971077.1 9398..10186(-) (pilF) [Acinetobacter towneri strain GX3]
MAQITTVCISAIFAAACQTTAPTLGQKDPEKAVQVRTQLAAEYLKNGDLDSAKRALDQALESDSRDSTANMMMGVLLQQE
GSKLNLEKADAYFKRAIAADPKNAQARNNYGTYLYQVERYNDAIEQLSIAGATLGYDQRFRALENVGRIYLKLGDIENAE
KSFKQALQANRDSYVSMLELSEILYLRQQFAPARQLYEQFVRAVGQKNQGARALWIGIRLARANGDQLGTQVLVNQLRAL
FPNSQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 789 bp        

>NTDB_id=547569 J4G44_RS00055 WP_371971077.1 9398..10186(-) (pilF) [Acinetobacter towneri strain GX3]
ATCGCACAAATTACAACTGTTTGTATTTCGGCAATTTTTGCTGCAGCTTGTCAAACCACAGCTCCAACCTTAGGGCAAAA
AGACCCTGAAAAAGCGGTGCAAGTACGTACGCAGTTGGCAGCAGAATACTTAAAAAATGGCGATTTAGACTCAGCCAAAC
GCGCACTTGATCAAGCTTTGGAAAGCGATTCACGTGATTCCACCGCCAATATGATGATGGGCGTGTTGCTGCAACAAGAA
GGCAGCAAGCTGAATTTAGAAAAAGCCGATGCGTATTTTAAGCGTGCCATTGCCGCTGACCCAAAAAATGCACAAGCGCG
TAACAACTATGGTACGTATTTATATCAAGTAGAACGCTATAATGATGCGATTGAGCAGCTCAGTATTGCAGGTGCAACAC
TGGGTTATGATCAACGCTTTAGAGCATTGGAAAATGTAGGTCGTATTTACTTGAAATTAGGCGATATAGAGAATGCCGAA
AAGTCATTCAAACAAGCCTTGCAAGCCAATCGCGATTCTTATGTTTCAATGTTAGAGTTGTCGGAAATTTTATATTTAAG
ACAACAATTTGCACCCGCGCGACAATTGTATGAACAATTTGTACGTGCTGTGGGGCAAAAAAATCAGGGTGCGCGTGCCT
TATGGATTGGGATTCGTTTAGCACGTGCCAATGGCGATCAATTGGGAACTCAGGTTTTAGTGAACCAGCTGCGTGCATTA
TTTCCCAATAGCCAAGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(93-125)

Tetratricopeptide repeat (TPR_16)

(39-76)

Tetratricopeptide repeat (TPR_16)

(147-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.778

96.183

0.748
  pilF Acinetobacter baylyi ADP1

72.047

96.947

0.698