Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HRE53_RS10100 Genome accession   NZ_CP071717
Coordinates   2184512..2186539 (+) Length   675 a.a.
NCBI ID   WP_212663327.1    Uniprot ID   -
Organism   Acaryochloris sp. 'Moss Beach'     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2179512..2191539
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HRE53_RS10085 (HRE53_09850) - 2180064..2181173 (+) 1110 WP_235110266.1 S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase -
  HRE53_RS10090 (HRE53_09855) - 2181178..2182518 (-) 1341 WP_235110268.1 TldD/PmbA family protein -
  HRE53_RS10095 (HRE53_09860) - 2182600..2184069 (-) 1470 WP_212663326.1 TldD/PmbA family protein -
  HRE53_RS10100 (HRE53_09865) pilB/pilB1 2184512..2186539 (+) 2028 WP_212663327.1 GspE/PulE family protein Machinery gene
  HRE53_RS10110 (HRE53_09870) - 2186584..2187760 (+) 1177 Protein_1973 glycosyltransferase family 4 protein -
  HRE53_RS10115 (HRE53_09875) - 2187862..2188479 (-) 618 WP_235110271.1 50S ribosomal protein L25/general stress protein Ctc -
  HRE53_RS10120 (HRE53_09880) - 2188848..2190023 (-) 1176 WP_235110273.1 glycosyltransferase family 4 protein -
  HRE53_RS10125 (HRE53_09885) - 2190106..2191002 (+) 897 WP_235110275.1 hypothetical protein -

Sequence

Protein


Download         Length: 675 a.a.        Molecular weight: 75369.66 Da        Isoelectric Point: 5.0388

>NTDB_id=547259 HRE53_RS10100 WP_212663327.1 2184512..2186539(+) (pilB/pilB1) [Acaryochloris sp. 'Moss Beach']
MENNSSKRELLVRNNFSPSNEKLIKSGYVDQARLREAIYESRRKGRSLLAILQKMTGKPLPPRLLRQIKRQQLFELKILY
GLDCLDPELTQVDFDEIDRLIRTVIPLDYCRRYQILPLACNDDVDPPVVRLGMVNPDHLSAIDDVDYLLRSNGMTFQRVV
LDLDTFQMLIAKFLDAQAKRQATIQENLPVEYGLEDDLDRLGGIEDLSTNDADINLDEALLDENAAPVISLVNKILAKAL
QNDISDIHIEPQEDNLRVRFRKDGLLHNAFAPLPRRIAPALVSRFKIIANLDIAERRLPQDGRILRLFQGRKVDFRVSTL
PSRYGEKVVLRILDNSAMQLGLNNLIDDEASLEIVREMVARPFGLILVTGPTGSGKTTTLYSALTERNTPEVNISTVEDP
IEYTLQGITQVQVLREKGMDFASILRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAIVRLAEMD
VEPFMVSASLLGVVAQRLVRRLCPDCRIPYRPSPEKLAQFGLMASAKTEVTFYKANTSTSDDILATANTEDACPTCAGIG
YSGRCGVYEVMQVTEKLKGLITEGAPTEHIKEVAVEEGMQTLLAYSLNLVEKGATSLEEVERVTFSDSGLKTELKAKRKT
ALNCLVCNAGLLPEWLDCPYCLTPRFAEHNPANQL

Nucleotide


Download         Length: 2028 bp        

>NTDB_id=547259 HRE53_RS10100 WP_212663327.1 2184512..2186539(+) (pilB/pilB1) [Acaryochloris sp. 'Moss Beach']
ATGGAGAATAACTCATCCAAGCGGGAACTGCTCGTTAGAAACAATTTTTCTCCTTCAAACGAAAAATTAATTAAGAGTGG
CTATGTAGATCAAGCACGCTTGCGAGAAGCGATTTATGAAAGTCGGCGAAAGGGACGCTCGCTACTAGCGATTCTCCAGA
AGATGACGGGGAAACCTTTGCCTCCCCGGCTTCTCCGTCAGATTAAGCGTCAGCAGCTATTTGAACTCAAAATTCTCTAT
GGGCTGGATTGCCTCGATCCTGAGCTCACCCAGGTTGACTTTGACGAAATTGATCGACTGATTCGCACGGTCATCCCCCT
CGACTATTGTCGTCGCTATCAGATTTTGCCCCTAGCCTGCAATGATGATGTCGATCCACCTGTTGTCCGGCTGGGGATGG
TCAACCCAGATCACCTCAGTGCCATTGATGATGTTGACTATTTGCTGCGCTCCAATGGAATGACCTTTCAGCGGGTTGTT
CTGGATTTGGATACCTTTCAAATGCTGATTGCCAAATTCTTAGACGCTCAAGCCAAACGTCAGGCAACCATCCAAGAAAA
TCTGCCCGTCGAATACGGACTGGAAGATGATCTAGATCGCCTAGGAGGAATTGAAGATCTATCTACCAATGATGCAGATA
TCAATCTAGATGAGGCCCTTCTAGATGAAAATGCGGCACCCGTCATTTCTTTAGTCAATAAGATATTGGCAAAAGCGTTG
CAAAACGACATTTCTGATATTCATATTGAGCCGCAAGAAGACAATTTGCGCGTTCGCTTTCGTAAAGACGGCCTACTGCA
CAATGCCTTTGCACCCCTACCACGTCGCATTGCCCCCGCGTTAGTCTCTCGTTTCAAGATCATCGCTAACCTAGATATTG
CGGAACGGCGTTTACCCCAAGATGGCCGGATTCTTCGGTTATTTCAAGGCCGCAAAGTTGACTTTCGAGTCAGCACCTTA
CCCAGCCGCTATGGCGAGAAGGTGGTTTTGCGGATTTTAGACAACTCTGCCATGCAGTTGGGTCTCAACAACCTAATTGA
CGATGAAGCCAGCCTTGAGATTGTACGGGAAATGGTAGCTCGCCCGTTTGGGTTGATTTTGGTTACAGGCCCCACCGGTT
CTGGTAAAACGACGACTCTATATTCAGCCTTAACTGAGCGTAATACGCCTGAGGTGAATATCAGTACCGTTGAAGATCCC
ATTGAATATACCCTGCAGGGGATTACCCAGGTTCAAGTCCTGCGGGAAAAAGGCATGGACTTCGCATCCATTCTGCGGGC
CTTTCTGCGACAAGACCCGGATGTCCTACTTGTGGGTGAGACTCGGGACAAGGAAACTGCAAAAACGGCGATTGAGGCAG
CGTTAACTGGTCACTTGGTGTTAACGACCTTGCACACCAACGATGCAGCCAGCGCCATTGTGCGTTTGGCAGAGATGGAT
GTGGAACCGTTTATGGTCTCGGCGTCCTTATTGGGTGTGGTTGCCCAGCGACTGGTTCGTCGCCTTTGTCCAGACTGTCG
GATTCCCTATCGTCCTTCCCCAGAAAAGCTGGCTCAGTTCGGGTTAATGGCTTCCGCCAAAACGGAGGTCACCTTTTACA
AAGCAAATACCTCCACGTCAGACGATATTTTGGCAACGGCAAACACCGAAGACGCTTGCCCCACCTGTGCTGGCATCGGT
TACTCCGGGCGATGCGGGGTCTATGAAGTCATGCAGGTAACTGAAAAGTTAAAGGGACTAATTACCGAAGGAGCCCCGAC
AGAGCACATCAAAGAAGTAGCTGTCGAAGAAGGGATGCAAACCTTGCTAGCCTACAGCCTGAATCTGGTTGAAAAAGGCG
CAACATCCCTAGAAGAGGTAGAACGAGTCACCTTCTCCGATAGTGGATTGAAAACAGAGCTAAAAGCGAAGCGCAAGACC
GCACTGAACTGTCTGGTTTGCAATGCAGGGCTCTTACCGGAGTGGTTGGACTGTCCTTACTGCCTGACGCCCAGATTTGC
AGAGCATAATCCTGCCAATCAACTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(61-178)

Type II/IV secretion system protein (T2SSE)

(234-501)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

59.789

98.37

0.588