Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   J1899_RS15040 Genome accession   NZ_CP071709
Coordinates   3029581..3030651 (-) Length   356 a.a.
NCBI ID   WP_214474975.1    Uniprot ID   -
Organism   Cytobacillus gottheilii strain 1839     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3024581..3035651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J1899_RS15005 (J1899_15010) - 3025550..3026347 (+) 798 WP_214474969.1 YqhG family protein -
  J1899_RS15010 (J1899_15015) - 3026408..3026593 (-) 186 WP_066443687.1 YqzE family protein -
  J1899_RS15015 (J1899_15020) comGG 3026707..3027087 (-) 381 WP_066443683.1 competence type IV pilus minor pilin ComGG -
  J1899_RS15020 (J1899_15025) comGF 3027084..3027557 (-) 474 WP_082851648.1 competence type IV pilus minor pilin ComGF -
  J1899_RS15025 (J1899_15030) comGD 3027809..3028249 (-) 441 WP_214474973.1 competence type IV pilus minor pilin ComGD -
  J1899_RS15030 (J1899_15035) comGC 3028246..3028563 (-) 318 WP_066443675.1 competence type IV pilus major pilin ComGC -
  J1899_RS15035 (J1899_15040) comGB 3028563..3029597 (-) 1035 WP_343216722.1 competence type IV pilus assembly protein ComGB -
  J1899_RS15040 (J1899_15045) comGA 3029581..3030651 (-) 1071 WP_214474975.1 competence type IV pilus ATPase ComGA Machinery gene
  J1899_RS15045 (J1899_15050) - 3030855..3031556 (+) 702 WP_214474977.1 helix-turn-helix domain-containing protein -
  J1899_RS15050 (J1899_15055) - 3031639..3031884 (+) 246 WP_066443663.1 DUF2626 domain-containing protein -
  J1899_RS15055 (J1899_15060) - 3032049..3032684 (-) 636 WP_214474980.1 MBL fold metallo-hydrolase -
  J1899_RS15060 (J1899_15065) - 3033101..3033274 (+) 174 WP_066443657.1 DUF2759 domain-containing protein -
  J1899_RS15065 (J1899_15070) - 3033320..3033634 (-) 315 WP_066443655.1 MTH1187 family thiamine-binding protein -
  J1899_RS15070 (J1899_15075) - 3033652..3034812 (-) 1161 WP_214474982.1 hypothetical protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 39995.08 Da        Isoelectric Point: 8.3290

>NTDB_id=547206 J1899_RS15040 WP_214474975.1 3029581..3030651(-) (comGA) [Cytobacillus gottheilii strain 1839]
MSNIERLAESIIRDAVQKQATDIHILPRKNDTLIQFRLASKLVPRIQLPREDCDRLISHFKFTASMDIGEKRRPQNGASS
YTMNGYQIGLRFSSLPSRFSESLVIRILPAHQNIPYYHISLFPETTRKMLALLKHAHGLIILTGPTGSGKTTTLYSLLNE
TSHIYNRNVITLEDPIEKENDQVMQVQVNEKAGVSYATGLKAILRHDPDIIMVGEIRDAETAQIAVRASLTGHLVLTTMH
TRDAQGAIVRLQEFGVSLLEIQQTLIAVTAQRLVELTCPYCGGECSPYCLSYGQLKRATIFELLTGKALHSVINHMNDGG
TLVEYRTLKDYIRKGIALGYVKGSEYERWVMDDAKE

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=547206 J1899_RS15040 WP_214474975.1 3029581..3030651(-) (comGA) [Cytobacillus gottheilii strain 1839]
GTGAGCAATATTGAAAGGCTCGCAGAAAGTATTATCCGTGATGCTGTCCAAAAACAGGCGACAGATATTCATATTCTTCC
CCGCAAAAACGATACATTGATTCAGTTTCGCTTAGCCAGCAAACTTGTCCCCAGAATTCAATTGCCAAGAGAGGATTGTG
ACCGTCTCATCTCTCACTTTAAATTCACTGCATCCATGGATATCGGTGAAAAGAGACGTCCGCAAAATGGAGCCAGCTCT
TACACGATGAATGGTTATCAGATTGGCTTGCGCTTTTCCAGTCTACCTTCCCGGTTTTCAGAAAGTCTTGTTATCCGCAT
CCTTCCCGCACATCAGAATATCCCTTACTATCACATTTCATTATTCCCTGAGACGACAAGAAAAATGCTGGCACTATTAA
AACATGCTCATGGACTGATTATTTTAACAGGTCCTACAGGCAGCGGCAAAACAACAACCTTATACTCACTCCTCAACGAA
ACCTCACATATCTACAATCGAAATGTAATCACATTAGAAGACCCAATTGAAAAAGAAAATGATCAGGTTATGCAGGTGCA
GGTAAATGAAAAAGCAGGAGTTTCTTATGCCACCGGTTTAAAGGCCATTTTGCGCCATGATCCGGATATTATTATGGTTG
GAGAAATTAGGGATGCTGAAACTGCTCAAATAGCGGTGCGAGCTTCGTTGACAGGACATTTAGTATTAACAACGATGCAC
ACAAGAGATGCACAGGGAGCGATTGTAAGACTGCAGGAGTTTGGTGTGAGTTTACTGGAAATTCAGCAAACACTGATTGC
TGTTACAGCTCAGCGGCTAGTGGAGCTGACTTGCCCTTATTGCGGGGGAGAGTGTTCTCCTTATTGCTTGAGTTATGGAC
AGTTAAAACGTGCTACTATTTTTGAACTTTTAACAGGGAAAGCGCTGCATAGTGTCATCAATCATATGAATGATGGAGGA
ACATTAGTTGAATATCGGACATTGAAGGATTATATCCGCAAAGGAATTGCCCTTGGATATGTAAAGGGATCGGAATATGA
ACGGTGGGTGATGGATGATGCAAAAGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

53.977

98.876

0.534
  pilB Glaesserella parasuis strain SC1401

36.857

98.315

0.362