Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   BKM86_RS05565 Genome accession   NZ_CP071585
Coordinates   1073089..1074048 (+) Length   319 a.a.
NCBI ID   WP_002852903.1    Uniprot ID   A0A5Y6P8U5
Organism   Campylobacter jejuni subsp. jejuni strain GB26     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1055402..1078092 1073089..1074048 within 0


Gene organization within MGE regions


Location: 1055402..1078092
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKM86_RS05480 (BKM86_05480) pglI 1055402..1056331 (-) 930 WP_002852770.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  BKM86_RS05485 (BKM86_05485) pglH 1056324..1057403 (-) 1080 WP_010891911.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  BKM86_RS05490 (BKM86_05490) pglK 1057400..1059094 (-) 1695 WP_052806162.1 ABC-type lipopolysaccharide transporter PglK -
  BKM86_RS05495 (BKM86_05495) galE 1059088..1060074 (-) 987 WP_002852683.1 UDP-glucose 4-epimerase GalE -
  BKM86_RS05500 (BKM86_05500) - 1060132..1060926 (-) 795 WP_002852820.1 3'-5' exonuclease -
  BKM86_RS05505 (BKM86_05505) waaC 1060993..1062021 (+) 1029 WP_002852873.1 lipopolysaccharide heptosyltransferase I -
  BKM86_RS05510 (BKM86_05510) htrB 1062011..1062898 (+) 888 WP_002864916.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  BKM86_RS05515 (BKM86_05515) - 1062895..1064442 (+) 1548 WP_052806163.1 glycosyltransferase -
  BKM86_RS05520 (BKM86_05520) - 1064446..1065639 (+) 1194 WP_052806164.1 glycosyltransferase family 2 protein -
  BKM86_RS05525 (BKM86_05525) - 1065688..1066755 (+) 1068 WP_002868608.1 beta-1,4-N-acetylgalactosaminyltransferase -
  BKM86_RS05530 (BKM86_05530) - 1066727..1067620 (-) 894 WP_002859246.1 glycosyltransferase family 2 protein -
  BKM86_RS05535 (BKM86_05535) - 1067709..1068587 (+) 879 WP_042635873.1 alpha-2,3-sialyltransferase -
  BKM86_RS05540 (BKM86_05540) neuB 1068588..1069628 (+) 1041 WP_032585995.1 N-acetylneuraminate synthase -
  BKM86_RS05545 (BKM86_05545) neuC 1069625..1070743 (+) 1119 WP_052806166.1 UDP-N-acetylglucosamine 2-epimerase -
  BKM86_RS05550 (BKM86_05550) - 1070740..1071405 (+) 666 WP_052806167.1 acylneuraminate cytidylyltransferase family protein -
  BKM86_RS05555 (BKM86_05555) - 1071393..1072226 (+) 834 WP_002878819.1 CatB-related O-acetyltransferase -
  BKM86_RS05560 (BKM86_05560) waaV 1072218..1073030 (-) 813 WP_002878817.1 glycosyltransferase family 2 protein -
  BKM86_RS05565 (BKM86_05565) waaF 1073089..1074048 (+) 960 WP_002852903.1 lipopolysaccharide heptosyltransferase II Regulator
  BKM86_RS05570 (BKM86_05570) gmhA 1074029..1074589 (-) 561 WP_002852775.1 D-sedoheptulose 7-phosphate isomerase -
  BKM86_RS05575 (BKM86_05575) rfaE1 1074586..1075971 (-) 1386 WP_002852777.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  BKM86_RS05580 (BKM86_05580) rfaD 1075964..1076917 (-) 954 WP_002852832.1 ADP-glyceromanno-heptose 6-epimerase -
  BKM86_RS05585 (BKM86_05585) gmhB 1076934..1077479 (-) 546 Protein_1092 HAD family hydrolase -
  BKM86_RS05590 (BKM86_05590) - 1077561..1077863 (+) 303 WP_002852762.1 cytochrome c -
  BKM86_RS05595 (BKM86_05595) ccoS 1077886..1078092 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -

Sequence

Protein


Download         Length: 319 a.a.        Molecular weight: 36766.44 Da        Isoelectric Point: 10.4054

>NTDB_id=546641 BKM86_RS05565 WP_002852903.1 1073089..1074048(+) (waaF) [Campylobacter jejuni subsp. jejuni strain GB26]
MKIFIHLPTWLGDAVMASPALYTIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLRKKLGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKYKEEHQVLKYLYFIENSLSIKAHFKDLKLPFKLKFQNPLVLKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHLSAVYKVKTVAIFGPTKFTQTSPWQNQNAKLVHLDLACMPCMQKICPLKHHKCMKDLKPQRVIEEARNLLKNPHL

Nucleotide


Download         Length: 960 bp        

>NTDB_id=546641 BKM86_RS05565 WP_002852903.1 1073089..1074048(+) (waaF) [Campylobacter jejuni subsp. jejuni strain GB26]
ATGAAAATTTTTATACACCTTCCCACTTGGCTAGGCGATGCGGTGATGGCTTCACCTGCTTTATACACCATAAAAGAACA
TTTTAAAAATGCTCAGTTTATCCTTTATGGATCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCCCGCTATAAACAAGCCCTATCTTTACGCAAAAAACTTGGTAAAATCGATTTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTGCATATTCTTAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGTA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTAGTATAAAAGCTCATTTTAAAGACTTAA
AACTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTGTCTTAAAAAATGGCAAAAAAATTCTAGGACTCAACCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAGATTTT
AATCTTTGGTGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGTCCTATGCACCTAAGTGCGGTTTATAAGGTAAAAACCGTAGCTATTTTTGGCCCCACAAAATTTACCCAAACTTCACC
TTGGCAAAATCAAAACGCAAAATTAGTACACTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAATCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATTTAAAGCCTCAAAGGGTTATAGAAGAAGCTAGAAATTTACTTAAAAACCCTCATCTATAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(96-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Y6P8U5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

94.984

100

0.95