Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   J3Q17_RS05750 Genome accession   NZ_CP071576
Coordinates   1122456..1123415 (+) Length   319 a.a.
NCBI ID   WP_002856368.1    Uniprot ID   -
Organism   Campylobacter jejuni strain R12     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1095306..1149284 1122456..1123415 within 0


Gene organization within MGE regions


Location: 1095306..1149284
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J3Q17_RS05625 (J3Q17_05625) pglG 1095306..1096199 (-) 894 WP_002868951.1 PDC sensor domain-containing protein -
  J3Q17_RS05630 (J3Q17_05630) pglF 1096199..1097971 (-) 1773 WP_002853720.1 UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining) -
  J3Q17_RS05635 (J3Q17_05635) pglE 1097973..1099133 (-) 1161 WP_207498254.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -
  J3Q17_RS05640 (J3Q17_05640) pglD 1099231..1099821 (-) 591 WP_002853531.1 UDP-N-acetylbacillosamine N-acetyltransferase -
  J3Q17_RS05645 (J3Q17_05645) pglC 1099808..1100410 (-) 603 WP_207498256.1 sugar transferase -
  J3Q17_RS05650 (J3Q17_05650) pglA 1100403..1101533 (-) 1131 WP_002854036.1 N, N'-diacetylbacillosaminyl-diphospho-undecaprenol alpha-1,3-N-acetylgalactosaminyltransferase -
  J3Q17_RS05655 (J3Q17_05655) pglB 1101543..1103684 (-) 2142 WP_207498258.1 STT3 domain-containing protein -
  J3Q17_RS05660 (J3Q17_05660) pglJ 1103688..1104785 (-) 1098 WP_002853444.1 N-acetylgalactosamine-N, N'-diacetylbacillosaminyl-diphospho-undecaprenol 4-alpha-N-acetylgalactosaminyltransferase -
  J3Q17_RS05665 (J3Q17_05665) pglI 1104773..1105702 (-) 930 WP_002856262.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  J3Q17_RS05670 (J3Q17_05670) pglH 1105695..1106774 (-) 1080 WP_207498259.1 glycosyltransferase -
  J3Q17_RS05675 (J3Q17_05675) pglK 1106771..1108465 (-) 1695 WP_002856016.1 ABC-type lipopolysaccharide transporter PglK -
  J3Q17_RS05680 (J3Q17_05680) galE 1108459..1109445 (-) 987 WP_207498261.1 UDP-glucose 4-epimerase GalE -
  J3Q17_RS05685 (J3Q17_05685) - 1109503..1110297 (-) 795 WP_002856546.1 3'-5' exonuclease -
  J3Q17_RS05690 (J3Q17_05690) waaC 1110363..1111391 (+) 1029 WP_002855693.1 lipopolysaccharide heptosyltransferase I -
  J3Q17_RS05695 (J3Q17_05695) htrB 1111381..1112268 (+) 888 WP_002855840.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  J3Q17_RS05700 (J3Q17_05700) - 1112265..1113813 (+) 1549 Protein_1117 glycosyltransferase -
  J3Q17_RS05705 (J3Q17_05705) - 1113817..1114986 (+) 1170 WP_002852863.1 glycosyltransferase family 2 protein -
  J3Q17_RS05710 (J3Q17_05710) - 1115056..1116099 (+) 1044 WP_002912176.1 beta-1,4-N-acetylgalactosaminyltransferase -
  J3Q17_RS05715 (J3Q17_05715) - 1116085..1116990 (-) 906 WP_002852740.1 glycosyltransferase family 2 protein -
  J3Q17_RS05720 (J3Q17_05720) - 1117079..1117954 (+) 876 WP_002869021.1 alpha-2,3-sialyltransferase -
  J3Q17_RS05725 (J3Q17_05725) neuB 1117955..1118995 (+) 1041 WP_002870496.1 N-acetylneuraminate synthase -
  J3Q17_RS05730 (J3Q17_05730) neuC 1118992..1120110 (+) 1119 WP_002911676.1 UDP-N-acetylglucosamine 2-epimerase -
  J3Q17_RS05735 (J3Q17_05735) - 1120107..1120772 (+) 666 WP_002855897.1 acylneuraminate cytidylyltransferase family protein -
  J3Q17_RS05740 (J3Q17_05740) - 1120760..1121593 (+) 834 WP_002855772.1 CatB-related O-acetyltransferase -
  J3Q17_RS05745 (J3Q17_05745) waaV 1121585..1122397 (-) 813 WP_002856066.1 glycosyltransferase family 2 protein -
  J3Q17_RS05750 (J3Q17_05750) waaF 1122456..1123415 (+) 960 WP_002856368.1 lipopolysaccharide heptosyltransferase II Regulator
  J3Q17_RS05755 (J3Q17_05755) gmhA 1123396..1123956 (-) 561 WP_002855785.1 D-sedoheptulose 7-phosphate isomerase -
  J3Q17_RS05760 (J3Q17_05760) rfaE1 1123953..1125347 (-) 1395 WP_032581686.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  J3Q17_RS05765 (J3Q17_05765) rfaD 1125340..1126293 (-) 954 WP_207498263.1 ADP-glyceromanno-heptose 6-epimerase -
  J3Q17_RS05770 (J3Q17_05770) gmhB 1126294..1126854 (-) 561 WP_207498265.1 HAD family hydrolase -
  J3Q17_RS05775 (J3Q17_05775) - 1126936..1127238 (+) 303 WP_002852762.1 cytochrome c -
  J3Q17_RS05780 (J3Q17_05780) ccoS 1127261..1127467 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  J3Q17_RS05785 (J3Q17_05785) - 1127464..1129821 (-) 2358 WP_207498266.1 heavy metal translocating P-type ATPase -
  J3Q17_RS05790 (J3Q17_05790) rho 1129930..1131228 (+) 1299 WP_002853705.1 transcription termination factor Rho -
  J3Q17_RS05795 (J3Q17_05795) dnaX 1131232..1132761 (+) 1530 WP_002855902.1 DNA polymerase III subunit gamma/tau -
  J3Q17_RS05800 (J3Q17_05800) - 1132758..1133279 (-) 522 WP_002855871.1 DUF2165 family protein -
  J3Q17_RS05805 (J3Q17_05805) copA 1133351..1135450 (-) 2100 WP_207498268.1 cation-translocating P-type ATPase -
  J3Q17_RS05810 (J3Q17_05810) - 1135450..1135644 (-) 195 WP_002856180.1 cation transporter -
  J3Q17_RS05815 (J3Q17_05815) czcD 1135634..1136584 (-) 951 WP_002856089.1 CDF family cation-efflux transporter CzcD -
  J3Q17_RS05820 (J3Q17_05820) - 1136659..1136922 (-) 264 WP_002831611.1 zf-TFIIB domain-containing protein -
  J3Q17_RS05825 (J3Q17_05825) - 1136992..1137486 (-) 495 WP_002853757.1 threonine/serine exporter family protein -
  J3Q17_RS05830 (J3Q17_05830) - 1137483..1138259 (-) 777 WP_002853925.1 threonine/serine exporter ThrE family protein -
  J3Q17_RS05835 (J3Q17_05835) ldh 1138326..1139252 (+) 927 WP_002927732.1 L-lactate dehydrogenase -
  J3Q17_RS05840 (J3Q17_05840) - 1139249..1139851 (-) 603 WP_002855976.1 DedA family protein -
  J3Q17_RS05845 (J3Q17_05845) - 1139932..1140159 (-) 228 WP_002822505.1 hypothetical protein -
  J3Q17_RS05850 (J3Q17_05850) - 1140170..1141606 (-) 1437 WP_002855775.1 DUF3373 family protein -
  J3Q17_RS05855 (J3Q17_05855) ppi 1141770..1142252 (-) 483 WP_002822502.1 peptidylprolyl isomerase -
  J3Q17_RS05860 (J3Q17_05860) - 1142262..1142969 (-) 708 WP_002787769.1 YebC/PmpR family DNA-binding transcriptional regulator -
  J3Q17_RS05865 (J3Q17_05865) - 1143078..1143419 (+) 342 WP_002783013.1 multidrug efflux SMR transporter -
  J3Q17_RS05870 (J3Q17_05870) - 1143419..1143727 (+) 309 WP_002780599.1 multidrug efflux SMR transporter -
  J3Q17_RS05875 (J3Q17_05875) argS 1143745..1145337 (-) 1593 WP_207498270.1 arginine--tRNA ligase -
  J3Q17_RS05880 (J3Q17_05880) tatA 1145349..1145588 (-) 240 WP_002852866.1 twin-arginine translocase TatA/TatE family subunit -
  J3Q17_RS05885 (J3Q17_05885) gmk 1145590..1146213 (-) 624 WP_079254372.1 guanylate kinase -
  J3Q17_RS05890 (J3Q17_05890) - 1146210..1147838 (-) 1629 WP_207498272.1 highly acidic protein -
  J3Q17_RS05895 (J3Q17_05895) fliR 1147895..1148662 (-) 768 WP_002855878.1 flagellar biosynthetic protein FliR -
  J3Q17_RS05900 (J3Q17_05900) - 1148649..1149284 (-) 636 WP_002852807.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 319 a.a.        Molecular weight: 36719.17 Da        Isoelectric Point: 10.1941

>NTDB_id=546398 J3Q17_RS05750 WP_002856368.1 1122456..1123415(+) (waaF) [Campylobacter jejuni strain R12]
MKIFIHLPTWLGDAVMASPALYAIKEHFKDAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLRKELGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLDIKAHFKDLKLPFKLKFQNPLILKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHLSAVYKVKTVAIFGPTNFTQTSPWQNENAKLVHLDLACMPCMQKTCPLKHHKCMKDLKPQRVIEEARNLLKNSHL

Nucleotide


Download         Length: 960 bp        

>NTDB_id=546398 J3Q17_RS05750 WP_002856368.1 1122456..1123415(+) (waaF) [Campylobacter jejuni strain R12]
ATGAAAATTTTTATACACCTTCCAACTTGGCTAGGCGATGCGGTGATGGCTTCACCTGCTTTATACGCTATAAAAGAACA
CTTTAAAGATGCCCAGTTTATCCTTTATGGCTCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCCCGTTATAAACAAGCCCTATCTTTACGCAAAGAACTTGGTAAGATCGATTTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTACATATTCTCAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTGACATAAAAGCTCATTTTAAAGACTTAA
AACTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTATCTTAAAAAATGGCAAAAAAATTCTAGGACTCAACCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAAATTTT
AATCTTTGGCGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGTCCTATGCACCTAAGTGCGGTTTATAAGGTAAAAACCGTAGCTATTTTTGGCCCCACAAATTTTACCCAAACTTCACC
TTGGCAAAATGAAAACGCAAAATTAGTACATTTAGATCTAGCTTGCATGCCTTGTATGCAAAAAACCTGTCCTTTAAAAC
ACCACAAATGCATGAAAGATCTAAAGCCTCAAAGGGTTATAGAAGAGGCTAGAAATTTACTTAAAAACTCTCATCTATGA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(95-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

95.611

100

0.956