Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LOF66_RS14590 Genome accession   NZ_CP087564
Coordinates   2875178..2876671 (-) Length   497 a.a.
NCBI ID   WP_001040655.1    Uniprot ID   -
Organism   Escherichia coli strain CM2010-0578/1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2870178..2881671
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOF66_RS14565 (LOF66_14480) panM 2870412..2870795 (+) 384 WP_000778768.1 aspartate 1-decarboxylase autocleavage activator PanM -
  LOF66_RS14570 (LOF66_14485) livJ 2870983..2872086 (-) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  LOF66_RS14575 (LOF66_14490) rpoH 2872357..2873211 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  LOF66_RS14580 (LOF66_14495) ftsX 2873456..2874514 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  LOF66_RS14585 (LOF66_14500) ftsE 2874507..2875175 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  LOF66_RS14590 (LOF66_14505) pilA 2875178..2876671 (-) 1494 WP_001040655.1 signal recognition particle-docking protein FtsY Machinery gene
  LOF66_RS14595 (LOF66_14510) rsmD 2876821..2877417 (+) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  LOF66_RS14600 (LOF66_14515) yhhL 2877407..2877676 (+) 270 WP_001311191.1 DUF1145 family protein -
  LOF66_RS14605 (LOF66_14520) yhhM 2877679..2878038 (-) 360 WP_000042895.1 DUF2500 domain-containing protein -
  LOF66_RS14610 (LOF66_14525) yhhN 2878179..2878805 (+) 627 WP_000964718.1 lysoplasmalogenase -
  LOF66_RS14615 (LOF66_14530) zntA 2878879..2881077 (+) 2199 WP_274480354.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  LOF66_RS14620 (LOF66_14535) tusA 2881179..2881424 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 54528.32 Da        Isoelectric Point: 4.1560

>NTDB_id=546099 LOF66_RS14590 WP_001040655.1 2875178..2876671(-) (pilA) [Escherichia coli strain CM2010-0578/1]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELETALA
AEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADV
GVETTRKIITNLTEGASRKQLRDAEALYGQLKEEMGEILAKVDEPLNVEGKAPFVILMVGVNGVGKTTTIGKLARQFEQQ
GKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKKI
VRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDL
RPFKADDFIEALFARED

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=546099 LOF66_RS14590 WP_001040655.1 2875178..2876671(-) (pilA) [Escherichia coli strain CM2010-0578/1]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCCTCTGAACAAGCCGTTGAAG
AGCAGCCGCAAGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATTACCGACGAAGAGCTGGAAACGGCGCTGGCT
GCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCA
GGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCGGAT
TTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTG
GGTGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGACGCCGAGGCGCT
CTATGGCCAGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGT
TTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAGCAG
GGTAAATCGGTGATGCTGGCGGCGGGTGATACTTTCCGTGCAGCTGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCGCAA
CAATATTCCGGTGATTGCCCAGCATACCGGGGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAGCGC
GTAATATCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATC
GTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGC
GGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGAAAG
GCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGATTTG
CGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(293-493)

SRP54-type protein, helical bundle domain (SRP54_N)

(210-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.698

77.264

0.376