Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   J2N86_RS11490 Genome accession   NZ_CP071527
Coordinates   2604458..2605057 (-) Length   199 a.a.
NCBI ID   WP_252579606.1    Uniprot ID   -
Organism   Legionella lytica strain PCM 2298     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2599458..2610057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J2N86_RS11470 (J2N86_11480) aroB 2600652..2601755 (-) 1104 WP_252579603.1 3-dehydroquinate synthase -
  J2N86_RS11475 (J2N86_11485) aroK 2601742..2602269 (-) 528 WP_040534720.1 shikimate kinase AroK -
  J2N86_RS11480 (J2N86_11490) - 2602563..2603849 (-) 1287 Protein_2256 type IV pilus secretin PilQ -
  J2N86_RS11485 (J2N86_11495) - 2603960..2604457 (-) 498 WP_252579605.1 pilus assembly protein PilP -
  J2N86_RS11490 (J2N86_11500) pilO 2604458..2605057 (-) 600 WP_252579606.1 type 4a pilus biogenesis protein PilO Machinery gene
  J2N86_RS11495 (J2N86_11505) - 2605054..2605620 (-) 567 WP_252579607.1 PilN domain-containing protein -
  J2N86_RS11500 (J2N86_11510) pilM 2605607..2606617 (-) 1011 WP_252582447.1 type IV pilus assembly protein PilM -
  J2N86_RS11505 (J2N86_11515) - 2606783..2607913 (+) 1131 WP_252579608.1 hypothetical protein -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22970.97 Da        Isoelectric Point: 8.2500

>NTDB_id=546015 J2N86_RS11490 WP_252579606.1 2604458..2605057(-) (pilO) [Legionella lytica strain PCM 2298]
MSRIPFHELTLETIGQWPAAVKTGLLIGLSSLIVIMGYCLVIHSNFISYKELQAQEIALKADFEIKQRQMINLDAYRHQL
FKLKEQFTSLLKYLPTENELYGLLEEISKTGMVSGLKFLLFAPQPEEVHGFYTILPIKISIEGDYFQLASFLRRIVTMER
IVTFHDFRIKGGLSRCNQPACEDVLVMNVTAKVYRYRTQ

Nucleotide


Download         Length: 600 bp        

>NTDB_id=546015 J2N86_RS11490 WP_252579606.1 2604458..2605057(-) (pilO) [Legionella lytica strain PCM 2298]
ATGAGCCGTATCCCTTTTCACGAGCTCACTTTAGAAACCATAGGGCAGTGGCCAGCAGCAGTAAAAACAGGACTGTTAAT
TGGCTTGAGTAGTTTAATTGTTATTATGGGATATTGCTTGGTAATTCATAGTAATTTTATTAGTTACAAGGAGTTACAAG
CTCAAGAAATTGCTTTGAAGGCAGATTTTGAAATTAAGCAACGACAGATGATTAATTTAGATGCTTATCGTCATCAGCTG
TTTAAGTTGAAAGAACAGTTTACGTCCTTACTTAAGTACTTGCCCACAGAAAATGAACTTTATGGCTTATTAGAAGAGAT
TTCGAAAACGGGAATGGTTTCTGGTCTTAAGTTTCTCTTATTTGCACCTCAACCTGAAGAGGTACATGGGTTTTATACCA
TCTTGCCAATAAAAATTTCTATTGAGGGAGATTATTTTCAATTAGCTTCATTTCTAAGGCGAATAGTAACTATGGAGCGT
ATTGTTACTTTTCATGATTTTCGCATTAAAGGGGGGCTGTCACGTTGTAATCAACCAGCCTGTGAAGATGTGCTCGTGAT
GAATGTCACAGCTAAAGTATACCGCTACCGTACTCAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(50-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

55.276

100

0.553