Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I8J32_RS07295 Genome accession   NZ_CP071518
Coordinates   1670529..1671641 (-) Length   370 a.a.
NCBI ID   WP_200610195.1    Uniprot ID   A0A975AT40
Organism   Agrilutibacter solisilvae strain R19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1665529..1676641
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I8J32_RS17860 (I8J32_007265) - 1665928..1667154 (-) 1227 WP_200610184.1 (Fe-S)-binding protein -
  I8J32_RS07270 (I8J32_007270) rplM 1667314..1667742 (+) 429 WP_200610186.1 50S ribosomal protein L13 -
  I8J32_RS07275 (I8J32_007275) rpsI 1667755..1668147 (+) 393 WP_200610188.1 30S ribosomal protein S9 -
  I8J32_RS07285 (I8J32_007285) - 1668514..1669506 (+) 993 WP_200610192.1 fumarylacetoacetate hydrolase family protein -
  I8J32_RS07290 (I8J32_007290) maiA 1669625..1670284 (+) 660 WP_200610193.1 maleylacetoacetate isomerase -
  I8J32_RS07295 (I8J32_007295) pilU 1670529..1671641 (-) 1113 WP_200610195.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I8J32_RS07300 (I8J32_007300) - 1671790..1672980 (+) 1191 WP_207526847.1 DUF4398 domain-containing protein -
  I8J32_RS07305 (I8J32_007305) - 1673349..1673567 (+) 219 WP_200610199.1 YdcH family protein -
  I8J32_RS07310 (I8J32_007310) - 1673871..1675247 (+) 1377 WP_200610201.1 pyridoxal-phosphate dependent enzyme -
  I8J32_RS07315 (I8J32_007315) - 1675352..1676521 (+) 1170 WP_200610203.1 PLP-dependent aspartate aminotransferase family protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40888.16 Da        Isoelectric Point: 6.3735

>NTDB_id=545912 I8J32_RS07295 WP_200610195.1 1670529..1671641(-) (pilU) [Agrilutibacter solisilvae strain R19]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGKLYPLGNTGLPAGMVKKIAYSLMDEGQVPIFERDLELNMALALAEAGR
FRVNVFKQRGEVGMVIRAIRSVIPSIEELQLPQVLKDIIMAPRGLVLIVGSTGSGKSTTLASMIDHRNSNTSGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTLERI
LNFFHESAHKNVLMNLALNLRAVISQRLVVGVDGRRLPATEVLINTPMIRDLMRRGQVHEIKPAMEGSLQEGMESFDQCL
FRLYKEGRIEREEALRAADSRDGLDLKFRLSEGGTGEHDPYADVFASSGG

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=545912 I8J32_RS07295 WP_200610195.1 1670529..1671641(-) (pilU) [Agrilutibacter solisilvae strain R19]
ATGGACATCGGCTACTTTCTGAAGCTGATGACGGAAAAGAACGCGTCGGACATGTTCCTGACGACGGGCGCGCCGGTATA
CATCAAGGTGGAAGGCAAGCTTTATCCGCTGGGCAACACCGGCCTGCCGGCCGGCATGGTCAAGAAGATCGCCTATTCGC
TGATGGACGAGGGCCAGGTCCCGATCTTCGAACGCGACCTGGAGCTCAACATGGCCCTGGCGCTGGCCGAGGCCGGCCGG
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTGATCCGCGCGATCCGCAGCGTGATCCCGTCCATCGA
AGAGCTGCAGCTGCCGCAGGTGCTCAAGGACATCATCATGGCCCCGCGCGGCCTGGTGCTGATCGTCGGCTCCACCGGTT
CGGGCAAGTCGACCACGCTGGCCTCGATGATCGACCACCGCAACTCGAACACCTCGGGCCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACAAGCACAAGAAGTCGATCGTCAACCAGCGCGAAGTCGGCCTGGATACCCACGCCTTCCACAA
CGCGCTCAAGAACGCGATGCGCGAGGCGCCGGACGTCATCCTGATCGGCGAAATCCTCGACGCCACCACGATGGAGGCAG
CCATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACGCTCGAGCGCATC
CTCAACTTCTTCCACGAGTCGGCGCACAAGAACGTGCTGATGAACCTCGCGCTGAACCTGCGCGCGGTGATCTCCCAGCG
CCTGGTCGTGGGCGTCGACGGCCGCCGCCTGCCGGCGACCGAAGTGCTGATCAACACGCCGATGATCCGCGACCTCATGC
GCCGCGGCCAGGTCCACGAGATCAAGCCGGCGATGGAAGGCTCGCTGCAGGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGCCTGTACAAGGAAGGCCGCATCGAGCGCGAGGAAGCACTGCGCGCGGCCGATTCGCGCGACGGCCTGGACCTCAA
GTTCCGCCTGTCCGAGGGCGGTACCGGCGAGCACGATCCCTACGCGGACGTGTTCGCCAGCTCGGGCGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

97.027

0.497
  pilU Acinetobacter baylyi ADP1

49.153

95.676

0.47
  pilU Vibrio cholerae strain A1552

47.74

95.676

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.512

92.973

0.432
  pilT Pseudomonas aeruginosa PAK

41.716

91.351

0.381
  pilT Acinetobacter baumannii strain A118

41.42

91.351

0.378
  pilT Acinetobacter nosocomialis M2

41.42

91.351

0.378
  pilT Acinetobacter baumannii D1279779

41.42

91.351

0.378
  pilT Vibrio cholerae strain A1552

40.058

93.784

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.058

93.784

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.124

91.351

0.376
  pilT Acinetobacter baylyi ADP1

40.882

91.892

0.376
  pilT Neisseria gonorrhoeae MS11

39.481

93.784

0.37
  pilT Neisseria meningitidis 8013

39.481

93.784

0.37
  pilT Legionella pneumophila strain Lp02

40.237

91.351

0.368
  pilT Legionella pneumophila strain ERS1305867

40.237

91.351

0.368