Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JYB84_RS04800 Genome accession   NZ_CP071501
Coordinates   1125418..1126530 (+) Length   370 a.a.
NCBI ID   WP_207322300.1    Uniprot ID   A0A975ALT3
Organism   Shewanella cyperi strain FJAT-53720     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1120418..1131530
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JYB84_RS04765 (JYB84_04765) rdgB 1120572..1121171 (-) 600 WP_207322293.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  JYB84_RS04770 (JYB84_04770) - 1121270..1121704 (-) 435 WP_207322294.1 DUF4426 domain-containing protein -
  JYB84_RS04775 (JYB84_04775) yggU 1121782..1122069 (-) 288 WP_207322295.1 DUF167 family protein YggU -
  JYB84_RS04780 (JYB84_04780) - 1122069..1122617 (-) 549 WP_207322296.1 YggT family protein -
  JYB84_RS04785 (JYB84_04785) proC 1122657..1123472 (-) 816 WP_207322297.1 pyrroline-5-carboxylate reductase -
  JYB84_RS04790 (JYB84_04790) - 1123615..1124322 (-) 708 WP_207322298.1 YggS family pyridoxal phosphate-dependent enzyme -
  JYB84_RS04795 (JYB84_04795) pilT 1124358..1125395 (+) 1038 WP_207322299.1 type IV pilus twitching motility protein PilT Machinery gene
  JYB84_RS04800 (JYB84_04800) pilU 1125418..1126530 (+) 1113 WP_207322300.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JYB84_RS04805 (JYB84_04805) - 1126683..1127180 (+) 498 WP_407696025.1 glutathione peroxidase -
  JYB84_RS04810 (JYB84_04810) hemH 1127186..1128190 (+) 1005 WP_228290883.1 ferrochelatase -
  JYB84_RS04815 (JYB84_04815) ruvX 1128298..1128720 (-) 423 WP_207322302.1 Holliday junction resolvase RuvX -
  JYB84_RS04820 (JYB84_04820) - 1128728..1129291 (-) 564 WP_207322303.1 YqgE/AlgH family protein -
  JYB84_RS04825 (JYB84_04825) yciH 1129308..1129637 (-) 330 WP_207322304.1 stress response translation initiation inhibitor YciH -
  JYB84_RS04830 (JYB84_04830) - 1129798..1130139 (-) 342 WP_207322305.1 hypothetical protein -
  JYB84_RS04835 (JYB84_04835) - 1130359..1130916 (+) 558 WP_207323104.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41080.03 Da        Isoelectric Point: 6.1894

>NTDB_id=545692 JYB84_RS04800 WP_207322300.1 1125418..1126530(+) (pilU) [Shewanella cyperi strain FJAT-53720]
MDVRPFLKVMVERKASDLFITAGFPPSAKIDGELRPLAENAFNPAQALDFVESLMSDAQKKEFHETRECNFAFAAKELGR
FRVSAFWQRESPGCVMRRIETKIPEMDDLRLPAILKDLVMSKRGLIIMVGGTGTGKSTSLAAMVGYRNGHARGHILTIED
PVEFVHDHRQSIITQREVGIDTDSFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHHQLLFDLSLNLRGIVAQQLVPKADGTGRRAAIEILINTPRVSSIIQKNELHLLKDTMAKSNEQGMQTFDQA
LFNLYQEGEISYADALHHADSPNDLRLMIKLASKDSGGSGFLEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=545692 JYB84_RS04800 WP_207322300.1 1125418..1126530(+) (pilU) [Shewanella cyperi strain FJAT-53720]
ATGGATGTACGTCCGTTTCTGAAGGTGATGGTCGAACGCAAGGCTTCGGATCTGTTCATCACCGCAGGCTTCCCGCCCAG
CGCCAAGATAGACGGTGAGCTGCGGCCGCTGGCGGAAAATGCCTTCAACCCGGCCCAGGCGCTGGACTTTGTCGAGTCCT
TGATGTCTGACGCCCAGAAAAAAGAATTCCACGAAACCCGTGAATGTAACTTCGCCTTTGCGGCCAAGGAGCTGGGACGT
TTTCGTGTAAGTGCCTTCTGGCAGAGGGAATCTCCAGGTTGCGTGATGCGCCGCATCGAAACCAAGATCCCCGAGATGGA
CGATCTCAGGCTGCCGGCCATCCTCAAGGATCTGGTGATGAGCAAGCGCGGTCTTATCATCATGGTCGGTGGTACCGGCA
CGGGTAAGTCCACCTCGCTCGCCGCCATGGTGGGTTATCGCAACGGTCATGCCCGCGGTCATATTCTCACCATTGAAGAT
CCGGTGGAATTTGTTCACGACCACAGGCAAAGCATCATTACCCAGCGTGAGGTGGGCATAGACACCGACTCCTTCGACGC
GGCGCTGAAAAGCTCCCTGCGCCAGGCGCCGGATGTGATCCTTATCGGTGAGATCCGCAGCCAGGAAACCATGGAGTTCG
CCCTGTCGTTTGCCGAAACCGGTCACCTGTGTATGGCTACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATGCACCTGGTGCCTGAAAGCAAACATCATCAATTGCTGTTCGACCTGTCGCTGAACCTGCGCGGCATTGTGGCTCAGCA
GCTGGTACCCAAGGCCGACGGCACCGGCCGCCGGGCAGCCATCGAAATTCTGATCAATACCCCCAGGGTGTCGAGCATCA
TCCAGAAGAACGAGCTGCACCTGCTAAAAGATACCATGGCCAAGTCCAATGAACAGGGAATGCAGACCTTCGACCAGGCG
CTGTTCAACCTGTATCAGGAAGGTGAAATCAGTTACGCCGATGCGCTGCACCATGCAGACTCGCCCAACGACCTGCGCCT
GATGATCAAGCTCGCCAGCAAGGACTCCGGCGGTTCGGGCTTTTTGGAAGGTGTGACCCTGGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.838

100

0.6
  pilU Acinetobacter baylyi ADP1

60.845

95.946

0.584
  pilU Vibrio cholerae strain A1552

58.378

100

0.584
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.436

97.838

0.405
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.424

89.189

0.378
  pilT Vibrio cholerae strain A1552

42.424

89.189

0.378
  pilT Acinetobacter baumannii strain A118

40.058

92.432

0.37
  pilT Acinetobacter nosocomialis M2

40.058

92.432

0.37
  pilT Acinetobacter baumannii D1279779

40.058

92.432

0.37
  pilT Legionella pneumophila strain Lp02

41.088

89.459

0.368
  pilT Legionella pneumophila strain ERS1305867

41.088

89.459

0.368
  pilT Acinetobacter baylyi ADP1

39.181

92.432

0.362