Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   J1G54_RS03695 Genome accession   NZ_CP071491
Coordinates   678514..679776 (+) Length   420 a.a.
NCBI ID   WP_160404856.1    Uniprot ID   A0AAX1M6L4
Organism   Glaesserella parasuis strain GHP1807     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 673514..684776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J1G54_RS03670 (J1G54_03670) - 673708..676263 (-) 2556 WP_021119495.1 penicillin-binding protein 1A -
  J1G54_RS03675 (J1G54_03675) comA 676417..677094 (+) 678 WP_021113429.1 hypothetical protein Machinery gene
  J1G54_RS03680 (J1G54_03680) comB 677070..677591 (+) 522 WP_021113427.1 hypothetical protein Machinery gene
  J1G54_RS03685 (J1G54_03685) comC 677588..678118 (+) 531 WP_021113425.1 hypothetical protein Machinery gene
  J1G54_RS03690 (J1G54_03690) comD 678163..678504 (+) 342 WP_021114623.1 pilus assembly, PilP family protein Machinery gene
  J1G54_RS03695 (J1G54_03695) comE 678514..679776 (+) 1263 WP_160404856.1 type IV pilus secretin PilQ Machinery gene
  J1G54_RS03700 (J1G54_03700) nusB 679855..680268 (+) 414 WP_021116599.1 transcription antitermination factor NusB -
  J1G54_RS03705 (J1G54_03705) thiL 680277..681245 (+) 969 WP_021112073.1 thiamine-phosphate kinase -
  J1G54_RS03710 (J1G54_03710) - 681248..681718 (+) 471 WP_021113432.1 phosphatidylglycerophosphatase A -
  J1G54_RS03715 (J1G54_03715) - 681720..682349 (+) 630 WP_015940164.1 LysE family transporter -
  J1G54_RS03720 (J1G54_03720) trxA 682646..682960 (+) 315 WP_015940165.1 thioredoxin -
  J1G54_RS03725 (J1G54_03725) - 683358..684158 (+) 801 WP_062923923.1 IS256 family transposase, variant Zn-binding type -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 47038.92 Da        Isoelectric Point: 7.7191

>NTDB_id=545667 J1G54_RS03695 WP_160404856.1 678514..679776(+) (comE) [Glaesserella parasuis strain GHP1807]
MRYLFLLFFATFPVLANQQISLSIRNAPTAEIISYLAEETGKNITISDEIKDTKNFRVEKSHFDEILNSLIKTHQLNLKK
ENGIYYIHQAQEHKQHTTAQLVNALPKLITKTIKLHYSKASEVIESLTKGQGNLLSESGYLHFDDRSNSIIVKDSAASVK
NFTQLIESLDKPTEQIAIEARIVTISSEHLQQLGVRWGLFSPNENHYKLAGNLEGNGLTTNNLNVNFPVNPSASVALQIA
AINGRVLDLELTALESENNIEIIASPRLLTTDKKPASIKQGTEIPYAMYSKKEEITDIEFREAVLGLEVTPHISKQNQIL
LDLAISQNSPNNQINNTMVTIDKQEIKTQVLAKHGETIVLGGIFQHLIAKGEDKVPLLGSIPVIKRLFSQNRDRISKREL
VIFVTPYIVKSEKMGAEKQK

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=545667 J1G54_RS03695 WP_160404856.1 678514..679776(+) (comE) [Glaesserella parasuis strain GHP1807]
ATGCGTTATTTATTTCTGCTCTTCTTTGCTACTTTTCCTGTATTAGCAAACCAACAAATTTCACTTTCGATAAGAAATGC
CCCAACGGCAGAGATTATTAGTTATTTGGCGGAGGAAACGGGAAAGAATATTACAATTTCGGATGAAATTAAAGATACAA
AAAATTTCAGAGTAGAAAAAAGCCATTTTGATGAGATATTAAATAGTTTAATCAAAACACATCAATTAAATTTAAAAAAA
GAAAATGGCATTTACTATATTCATCAAGCCCAAGAACATAAACAACATACTACGGCACAATTAGTTAATGCTCTGCCTAA
ATTAATCACCAAAACAATCAAGTTACACTATTCCAAAGCCTCTGAAGTTATAGAGTCTTTAACAAAAGGGCAAGGCAATT
TACTATCAGAAAGTGGTTATCTTCACTTTGATGATCGCAGTAATAGCATTATCGTCAAAGACAGTGCCGCCTCAGTTAAA
AACTTTACTCAACTTATCGAAAGCCTAGATAAACCAACGGAACAAATTGCAATTGAAGCCCGAATTGTCACTATCAGCAG
TGAACATTTACAACAACTTGGCGTGCGTTGGGGCTTATTTTCCCCTAACGAAAATCACTACAAATTGGCAGGCAATTTAG
AAGGCAACGGATTAACTACCAACAACTTAAACGTAAATTTTCCAGTAAATCCGTCCGCTTCTGTTGCCTTACAAATTGCT
GCAATCAACGGTCGTGTACTTGATTTAGAACTTACTGCATTAGAAAGCGAAAATAACATAGAGATCATTGCAAGCCCTCG
CTTACTCACAACAGATAAAAAACCAGCGAGTATCAAACAAGGTACAGAAATTCCTTATGCAATGTACAGTAAGAAAGAGG
AAATCACCGATATTGAATTTCGTGAAGCCGTTTTGGGGTTAGAGGTCACGCCACATATTTCTAAACAAAATCAGATTTTG
TTAGACCTCGCTATCAGCCAAAATTCGCCCAATAACCAAATAAATAATACAATGGTGACAATTGATAAACAGGAAATCAA
AACGCAAGTTCTAGCTAAACACGGTGAAACCATCGTATTAGGCGGTATTTTTCAACATCTGATCGCCAAAGGCGAAGATA
AAGTACCGCTGTTAGGCAGTATCCCCGTGATTAAACGCTTATTTAGCCAAAATCGAGATAGAATCTCTAAACGAGAATTA
GTGATTTTTGTTACGCCTTATATTGTAAAATCTGAAAAAATGGGAGCGGAAAAGCAGAAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(253-410)

Bacterial type II/III secretion system short domain (Secretin_N)

(110-175)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

98.571

100

0.986
  comE Haemophilus influenzae Rd KW20

53.412

100

0.54
  comE Haemophilus influenzae 86-028NP

52.471

100

0.531
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

38.164

98.571

0.376
  pilQ Vibrio cholerae strain A1552

38.164

98.571

0.376
  pilQ Vibrio campbellii strain DS40M4

36.493

100

0.367