Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   J0P05_RS16190 Genome accession   NZ_CP071466
Coordinates   3180847..3181269 (+) Length   140 a.a.
NCBI ID   WP_013057949.1    Uniprot ID   A0A8D3X0F8
Organism   Priestia megaterium strain H2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3175847..3186269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0P05_RS16165 (J0P05_16165) - 3176086..3176907 (+) 822 WP_029322903.1 histidine kinase -
  J0P05_RS16170 (J0P05_16170) - 3176908..3177549 (+) 642 WP_097812226.1 response regulator transcription factor -
  J0P05_RS16175 (J0P05_16175) - 3177725..3178717 (+) 993 WP_029322907.1 glycerophosphodiester phosphodiesterase family protein -
  J0P05_RS16180 (J0P05_16180) - 3178830..3180212 (+) 1383 WP_207371621.1 PepSY domain-containing protein -
  J0P05_RS16185 (J0P05_16185) - 3180242..3180706 (+) 465 WP_013057948.1 FixH family protein -
  J0P05_RS16190 (J0P05_16190) nucA/comI 3180847..3181269 (+) 423 WP_013057949.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  J0P05_RS16195 (J0P05_16195) - 3181423..3181776 (+) 354 WP_223272376.1 DUF1516 family protein -
  J0P05_RS16200 (J0P05_16200) - 3181817..3182410 (-) 594 WP_013057951.1 hypothetical protein -
  J0P05_RS16205 (J0P05_16205) - 3182566..3183222 (-) 657 WP_013057952.1 TetR/AcrR family transcriptional regulator -
  J0P05_RS16210 (J0P05_16210) - 3183246..3184526 (-) 1281 WP_242647162.1 glycosyltransferase -
  J0P05_RS16215 (J0P05_16215) - 3184824..3185369 (+) 546 WP_013057954.1 GNAT family N-acetyltransferase -
  J0P05_RS16220 (J0P05_16220) - 3185669..3186154 (-) 486 WP_053487488.1 hypothetical protein -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15329.17 Da        Isoelectric Point: 4.4990

>NTDB_id=545541 J0P05_RS16190 WP_013057949.1 3180847..3181269(+) (nucA/comI) [Priestia megaterium strain H2]
MKKIVSGILILVLAVIVMIEGNPLEQNSSTNTSYDEVITFPADRYPETAAHIQHAIDKGKSAVCTIDRDGAEENRRESLK
GIPTKSGYDRDEFPMAFCEEGGSGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=545541 J0P05_RS16190 WP_013057949.1 3180847..3181269(+) (nucA/comI) [Priestia megaterium strain H2]
TTGAAAAAAATTGTTTCTGGTATTTTAATTTTAGTATTAGCCGTTATTGTTATGATTGAAGGAAATCCGTTAGAGCAGAA
CTCTTCAACGAATACCTCCTACGATGAAGTAATTACCTTCCCTGCTGATCGTTACCCTGAAACTGCAGCGCACATTCAAC
ATGCAATCGATAAAGGAAAATCGGCTGTTTGTACAATCGACCGAGACGGGGCTGAGGAAAACCGCCGTGAGTCTTTAAAA
GGCATTCCGACTAAAAGCGGCTATGACCGAGATGAGTTTCCGATGGCCTTTTGTGAAGAAGGCGGAAGCGGTGCCGATAT
TGAATATGTAAAGCCTTCAGACAATCGAGGCGCCGGGTCTTGGATCTCACATCAAGTAGACGAGTTTCCTGACGGTACGA
AGGTTTTAATTGAAGTGAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(43-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.207

82.857

0.507