Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   J0J33_RS03310 Genome accession   NZ_CP071295
Coordinates   644059..645420 (+) Length   453 a.a.
NCBI ID   WP_029345652.1    Uniprot ID   A0A252CD69
Organism   Lactococcus sp. LG1267     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 639059..650420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0J33_RS03275 (J0J33_03275) rpsM 639174..639539 (+) 366 WP_003133773.1 30S ribosomal protein S13 -
  J0J33_RS03280 (J0J33_03280) rpsK 639557..639940 (+) 384 WP_003133774.1 30S ribosomal protein S11 -
  J0J33_RS03285 (J0J33_03285) - 639992..640930 (+) 939 WP_003133775.1 DNA-directed RNA polymerase subunit alpha -
  J0J33_RS03290 (J0J33_03290) rplQ 640946..641326 (+) 381 WP_003133776.1 50S ribosomal protein L17 -
  J0J33_RS03295 (J0J33_03295) - 641716..643200 (+) 1485 WP_173816860.1 S-layer homology domain-containing protein -
  J0J33_RS03300 (J0J33_03300) - 643210..643662 (+) 453 WP_019292998.1 hypothetical protein -
  J0J33_RS03310 (J0J33_03310) radA 644059..645420 (+) 1362 WP_029345652.1 DNA repair protein RadA Machinery gene
  J0J33_RS03315 (J0J33_03315) - 645552..646628 (+) 1077 WP_019291422.1 PIN/TRAM domain-containing protein -
  J0J33_RS03320 (J0J33_03320) - 646651..647280 (+) 630 WP_019292996.1 pyridoxamine 5'-phosphate oxidase family protein -
  J0J33_RS03325 (J0J33_03325) ald 647467..648588 (+) 1122 WP_019291420.1 alanine dehydrogenase -
  J0J33_RS03330 (J0J33_03330) - 648619..649113 (-) 495 WP_019291419.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49754.94 Da        Isoelectric Point: 4.9237

>NTDB_id=544712 J0J33_RS03310 WP_029345652.1 644059..645420(+) (radA) [Lactococcus sp. LG1267]
MAKKKSTFLCQECGYKSVKKLGRCPNCGAWGSFVEEVEVQEVKNQRVSLTGEHSKPMKLDQVELFDTPRVETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDTDFYLYAETNMQSIRNEVE
RLQPDFLIVDSIQTIMTPEIQSTQGSVSQVREVTGELMQLAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
QNTFRILRAVKNRFGSTNEIGIFEMQGSGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRAGFLLQQQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEEPTDARECFIGEIGLTGEIR
RVTRMEQRLNEASKLGFQKVYAPKNSLAGVDIPENLQVIGVTTLSECLKKVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=544712 J0J33_RS03310 WP_029345652.1 644059..645420(+) (radA) [Lactococcus sp. LG1267]
ATAGCTAAAAAAAAATCAACCTTTCTTTGTCAAGAATGTGGCTATAAATCAGTAAAAAAACTCGGACGTTGTCCCAATTG
TGGCGCTTGGGGCTCCTTTGTGGAAGAAGTAGAAGTCCAAGAGGTAAAAAATCAGAGAGTAAGTTTGACAGGTGAGCACT
CTAAACCCATGAAGTTAGATCAGGTTGAACTTTTTGATACACCGCGCGTGGAAACCGACCTAGATGAGTTTAACCGAGTC
CTTGGAGGCGGTGTCGTTCCAGGAAGTCTTGTCCTTATTGGGGGCGATCCAGGGATTGGTAAATCGACTCTGCTGCTTCA
AGTATCTACACAACTTGCTTCAAGAGGGCGTGTGCTTTATGTGAGTGGGGAGGAGTCAGCTCAACAAATTAAGCTGCGTG
CTGAGCGTTTGGGAGACATTGATACAGATTTTTATCTTTATGCCGAAACCAATATGCAAAGTATCCGAAATGAAGTGGAA
CGTCTACAGCCAGATTTTCTTATTGTAGATTCGATTCAAACTATCATGACGCCTGAAATTCAGAGTACCCAGGGTTCAGT
TAGTCAAGTGCGCGAGGTGACAGGAGAATTAATGCAGTTGGCCAAAACTAACGATATTGCGACTTTTATTGTCGGACATG
TCACCAAGGAAGGGCAACTTGCAGGACCACGTATGTTGGAACATATGGTTGATACCGTCTTGTATTTTGAAGGAGAACGC
CAAAATACATTCCGAATTTTACGCGCGGTCAAAAACCGTTTTGGTTCAACAAATGAAATTGGAATTTTCGAGATGCAAGG
CAGTGGTTTAGTGGAAGTAACCAACCCGAGTGAAGTCTTTCTGGAAGAACGTCTAGAGGGTTCTACCGGATCTGCTATCG
TCTGTGCGTTAGAAGGTACTCGTCCGATATTGGTTGAAATTCAAGCTTTGACAACACCAACCATGTTTGGGAATGCCAAA
CGTACAACCTCCGGTTTAGACTTTAACCGTGTGAGTTTAATCATGGCAGTACTTGAAAAGCGTGCCGGTTTTCTTTTACA
ACAGCAAGATGCTTACTTGAAATCTGCAGGTGGGGTCAAGTTGGATGAACCAGCCATCGACTTGGCAGTCGCTGTGGCAA
TTGCTTCCAGCTATAAGGAAGAGCCAACAGATGCACGTGAATGCTTTATTGGCGAAATTGGCCTAACTGGAGAAATCCGA
CGTGTCACACGTATGGAGCAACGTCTCAATGAAGCAAGTAAATTAGGTTTCCAAAAAGTATATGCGCCTAAGAATAGCTT
AGCAGGTGTAGATATTCCAGAAAATTTGCAAGTGATCGGCGTGACGACTTTATCTGAATGTTTGAAAAAAGTGTTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(77-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(359-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A252CD69

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

80.795

100

0.808
  radA Streptococcus pneumoniae D39

80.795

100

0.808
  radA Streptococcus pneumoniae R6

80.795

100

0.808
  radA Streptococcus pneumoniae TIGR4

80.795

100

0.808
  radA Streptococcus mitis NCTC 12261

80.795

100

0.808
  radA Streptococcus mitis SK321

80.795

100

0.808
  radA Bacillus subtilis subsp. subtilis str. 168

61.438

100

0.623