Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   J0J32_RS00925 Genome accession   NZ_CP071285
Coordinates   172544..173905 (+) Length   453 a.a.
NCBI ID   WP_080567145.1    Uniprot ID   -
Organism   Lactococcus garvieae strain LG728     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 167544..178905
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0J32_RS00880 (J0J32_00880) - 168001..168723 (-) 723 WP_003133770.1 metal ABC transporter ATP-binding protein -
  J0J32_RS00885 (J0J32_00885) - 168745..169188 (-) 444 WP_003133771.1 zinc-dependent MarR family transcriptional regulator -
  J0J32_RS00890 (J0J32_00890) infA 169390..169608 (+) 219 WP_003133772.1 translation initiation factor IF-1 -
  J0J32_RS00895 (J0J32_00895) rpmJ 169662..169778 (+) 117 WP_014024193.1 50S ribosomal protein L36 -
  J0J32_RS00900 (J0J32_00900) rpsM 169796..170161 (+) 366 WP_003133773.1 30S ribosomal protein S13 -
  J0J32_RS00905 (J0J32_00905) rpsK 170179..170562 (+) 384 WP_003133774.1 30S ribosomal protein S11 -
  J0J32_RS00910 (J0J32_00910) - 170614..171552 (+) 939 WP_003133775.1 DNA-directed RNA polymerase subunit alpha -
  J0J32_RS00915 (J0J32_00915) rplQ 171568..171948 (+) 381 WP_003133776.1 50S ribosomal protein L17 -
  J0J32_RS00925 (J0J32_00925) radA 172544..173905 (+) 1362 WP_080567145.1 DNA repair protein RadA Machinery gene
  J0J32_RS00930 (J0J32_00930) - 174039..175115 (+) 1077 WP_003133779.1 PIN/TRAM domain-containing protein -
  J0J32_RS00935 (J0J32_00935) - 175138..175767 (+) 630 WP_003133780.1 pyridoxamine 5'-phosphate oxidase family protein -
  J0J32_RS00940 (J0J32_00940) ald 175955..177076 (+) 1122 WP_003133782.1 alanine dehydrogenase -
  J0J32_RS00945 (J0J32_00945) - 177110..177604 (-) 495 WP_003133783.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49768.92 Da        Isoelectric Point: 4.8676

>NTDB_id=544567 J0J32_RS00925 WP_080567145.1 172544..173905(+) (radA) [Lactococcus garvieae strain LG728]
MAKKKSTFLCQECGYKSVKKLGRCPNCGAWGSFVEEVEVQEVKNQRVSLTGEHSKPMKLDQVELFDTPRVETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDTDFYLYAETNMQSIRNEVE
RLQPDFLIVDSIQTIMTPEIQSTQGSVSQVREVTGELMQLAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
QNTFRILRAVKNRFGSTNEIGIFEMQGNGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRAGFLLQQQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEEPTDARECFIGEIGLTGEIR
RVTRMEQRLNEASKLGFQKVYAPKNSLAGVDIPENLEVVGVTTLSECLKKVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=544567 J0J32_RS00925 WP_080567145.1 172544..173905(+) (radA) [Lactococcus garvieae strain LG728]
ATAGCTAAAAAAAAATCAACATTTCTTTGCCAGGAATGTGGCTACAAATCAGTAAAAAAACTCGGACGTTGTCCCAATTG
TGGTGCTTGGGGTTCCTTTGTTGAAGAAGTAGAAGTCCAAGAGGTAAAAAATCAGAGGGTGAGTCTGACAGGCGAGCACT
CTAAACCCATGAAGTTAGATCAGGTTGAACTTTTCGATACACCGCGTGTGGAAACTGATCTGGATGAGTTTAACCGCGTC
CTTGGAGGCGGTGTCGTTCCAGGAAGTCTAGTCCTTATTGGGGGGGATCCAGGGATTGGTAAATCTACTCTACTGCTTCA
AGTATCTACGCAACTTGCTTCAAGAGGGCGTGTACTTTATGTCAGTGGGGAGGAGTCGGCTCAACAAATTAAGCTGCGTG
CCGAGCGTTTGGGCGACATCGATACAGATTTTTACCTTTACGCCGAAACCAATATGCAAAGCATCCGAAATGAAGTTGAA
CGTCTACAGCCAGACTTTCTTATTGTAGATTCGATTCAAACTATCATGACGCCTGAAATCCAGAGTACTCAGGGTTCGGT
TAGTCAGGTGCGCGAGGTGACAGGAGAATTAATGCAGTTGGCCAAAACCAACGATATTGCGACCTTTATTGTCGGACATG
TCACTAAGGAAGGGCAACTTGCGGGGCCACGTATGTTGGAACATATGGTTGATACGGTCTTGTATTTTGAGGGAGAACGT
CAAAATACATTCCGAATTTTACGCGCGGTCAAAAACCGTTTTGGTTCAACAAATGAAATTGGGATTTTCGAGATGCAGGG
CAATGGTTTAGTGGAAGTGACCAACCCGAGTGAAGTTTTTCTTGAGGAGCGTTTGGAAGGCTCTACGGGCTCTGCTATCG
TCTGTGCGTTAGAAGGTACGCGTCCGATATTGGTTGAGATTCAAGCTTTAACAACGCCAACCATGTTTGGAAATGCCAAA
CGTACAACCTCTGGTTTGGACTTTAACCGTGTGAGTTTAATCATGGCAGTACTCGAAAAGCGTGCCGGTTTTCTTTTGCA
ACAACAGGATGCCTACTTAAAATCCGCAGGTGGTGTCAAGTTGGATGAACCAGCCATTGACTTAGCAGTCGCTGTAGCTA
TTGCTTCTAGCTACAAGGAAGAGCCGACAGATGCACGCGAATGCTTTATTGGCGAAATTGGCTTGACGGGTGAAATTCGT
CGTGTTACGCGGATGGAGCAACGTCTCAATGAAGCAAGCAAACTAGGTTTCCAAAAAGTTTACGCGCCTAAGAATAGCTT
GGCAGGCGTAGATATTCCAGAAAATTTGGAAGTGGTCGGTGTGACGACTTTATCAGAATGTTTGAAAAAAGTATTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(77-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(359-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

80.353

100

0.804
  radA Streptococcus pneumoniae D39

80.353

100

0.804
  radA Streptococcus pneumoniae R6

80.353

100

0.804
  radA Streptococcus pneumoniae TIGR4

80.353

100

0.804
  radA Streptococcus mitis NCTC 12261

80.353

100

0.804
  radA Streptococcus mitis SK321

80.353

100

0.804
  radA Bacillus subtilis subsp. subtilis str. 168

61.438

100

0.623