Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   J0J70_RS04460 Genome accession   NZ_CP071250
Coordinates   912200..913588 (-) Length   462 a.a.
NCBI ID   WP_068759265.1    Uniprot ID   A0A0X1TKM2
Organism   Turicibacter bilis strain ISU324     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 907200..918588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0J70_RS04440 (J0J70_04455) - 907238..908776 (-) 1539 WP_212724563.1 FUSC family protein -
  J0J70_RS04445 (J0J70_04460) - 909046..910266 (+) 1221 WP_055305260.1 cation:proton antiporter -
  J0J70_RS04450 (J0J70_04465) - 910299..911318 (-) 1020 WP_256637859.1 DUF3221 domain-containing protein -
  J0J70_RS04455 (J0J70_04470) - 911334..912167 (-) 834 WP_212724562.1 DUF4349 domain-containing protein -
  J0J70_RS04460 (J0J70_04475) radA 912200..913588 (-) 1389 WP_068759265.1 DNA repair protein RadA Machinery gene
  J0J70_RS04465 (J0J70_04480) hflX 913821..915086 (+) 1266 WP_055275505.1 GTPase HflX -
  J0J70_RS04470 (J0J70_04485) - 915126..915683 (-) 558 WP_055275503.1 hypothetical protein -
  J0J70_RS04475 (J0J70_04490) - 915687..916196 (-) 510 WP_055243497.1 RNA polymerase sigma factor -
  J0J70_RS04480 (J0J70_04495) - 916473..916802 (-) 330 WP_055243496.1 hypothetical protein -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 50945.81 Da        Isoelectric Point: 7.9828

>NTDB_id=544307 J0J70_RS04460 WP_068759265.1 912200..913588(-) (radA) [Turicibacter bilis strain ISU324]
MAKARTKTSFFCQQCGTESLKWVGRCPGCGEWNTMVEELKPTKAEQRRGFVASESMAKPQRLQEIETKEEARIQTSMSEL
NRVLGGGIVRGSLVLCGGEPGIGKSTLLLQTAQDLAHKNVQVLYVSGEESARQIKLRAERLGVSSEHLYIYAETDLTLIE
RQIQQLKPEFVIIDSIQTIHMPEVTSAPGSVSQVRECTAQLMKIAKIGGISVFIVGHVTKDGNIAGPRLLEHMVDTVLYF
EGDRHHTYRILRAVKNRFGSTNEIGIFDMKEEGLDEVTNASEVFLEDRTKGLPGTAIMASIEGTRPILVEIQSLLTPTSF
GNPKRMASGVDHNRVSLILAVLEKRMGFFLQNQDTYVKVTGGVKLDEPAVDLAIVASIVSSYKDKMTPSGDVYMGEVGLT
GEIRRVSRIEERVKEAKKLGFTRAIIPKKNIGGWTAPKGMEIVGVDTISDALKAMFKDDLKF

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=544307 J0J70_RS04460 WP_068759265.1 912200..913588(-) (radA) [Turicibacter bilis strain ISU324]
ATGGCGAAAGCAAGAACAAAAACATCATTTTTCTGTCAGCAATGTGGGACAGAAAGTTTAAAATGGGTCGGAAGATGTCC
AGGTTGTGGCGAATGGAATACGATGGTGGAGGAATTAAAGCCCACAAAGGCAGAACAGCGCCGTGGCTTTGTAGCAAGTG
AAAGTATGGCAAAACCTCAACGTCTACAAGAGATAGAAACAAAAGAGGAAGCACGTATTCAGACATCAATGAGTGAGCTT
AACCGTGTTCTTGGTGGTGGAATTGTTCGTGGATCACTTGTTCTGTGTGGGGGTGAGCCTGGGATTGGGAAATCAACACT
ACTTTTACAAACGGCACAAGATTTAGCGCATAAAAATGTTCAAGTATTATATGTTTCAGGAGAAGAATCTGCTCGTCAAA
TTAAACTTCGTGCTGAGCGTTTAGGAGTAAGTAGCGAGCACTTATATATTTACGCAGAAACAGATTTAACTTTAATTGAA
CGTCAAATTCAACAGTTAAAACCAGAGTTTGTCATTATCGACTCGATTCAGACGATTCATATGCCAGAGGTAACATCGGC
ACCTGGGAGTGTCTCGCAAGTTCGTGAATGTACGGCCCAATTGATGAAAATTGCTAAAATTGGTGGAATTTCAGTCTTTA
TTGTCGGACATGTGACGAAAGATGGAAATATCGCTGGGCCACGTTTACTCGAGCATATGGTGGATACTGTCTTATATTTT
GAAGGAGATCGTCATCATACGTATCGTATTTTACGAGCGGTTAAAAACCGATTTGGGTCAACGAATGAGATTGGAATCTT
TGATATGAAAGAAGAGGGGCTTGATGAAGTGACGAATGCCTCAGAGGTCTTCTTAGAAGATCGCACGAAAGGATTACCAG
GAACGGCGATTATGGCCTCAATTGAAGGAACACGTCCAATCTTAGTTGAGATTCAATCATTATTAACGCCGACTTCATTT
GGTAATCCAAAACGAATGGCATCTGGAGTGGATCATAATCGTGTATCTTTGATTTTAGCTGTGCTTGAAAAACGTATGGG
ATTCTTCTTACAAAATCAAGATACATATGTAAAAGTAACTGGTGGTGTAAAGTTAGATGAACCGGCTGTCGATTTAGCGA
TTGTGGCAAGTATTGTTTCAAGTTATAAAGATAAGATGACGCCAAGTGGCGATGTATATATGGGAGAGGTTGGATTAACG
GGTGAAATTCGACGTGTGTCTCGTATTGAAGAGCGTGTGAAAGAAGCGAAAAAACTTGGGTTTACGCGCGCAATTATTCC
AAAGAAAAATATAGGTGGATGGACAGCTCCAAAAGGAATGGAGATTGTTGGGGTTGATACCATTTCGGATGCACTTAAAG
CAATGTTTAAAGATGATTTGAAGTTCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(61-222)

Subunit ChlI of Mg-chelatase (ChlI)

(346-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(11-37)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0X1TKM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.265

97.835

0.639
  radA Streptococcus mitis NCTC 12261

58.72

98.052

0.576
  radA Streptococcus pneumoniae Rx1

58.72

98.052

0.576
  radA Streptococcus pneumoniae D39

58.72

98.052

0.576
  radA Streptococcus pneumoniae R6

58.72

98.052

0.576
  radA Streptococcus pneumoniae TIGR4

58.72

98.052

0.576
  radA Streptococcus mitis SK321

58.499

98.052

0.574